Abstract: Objective: Investigating the protective effect of MVE was the purpose of this study, which performed on LPS-induced liver injury in aged mice. Methods: The mice were randomized into the groups of normal control, MVE, LPS and LPS+MVE. The pathological changes of liver tissue and the expression of myeloperoxidase were observed by hematoxylin-eosin staining and immunohistochemical staining. The levels of IL-1β, IL-17, CXCL1, CXCL17 and other factors in liver were measured. Western blotting was used to detect the expressions of TLR4 and NF-κB p65 in liver tissue samples. Results: The MVE could effectively decline the infiltration of neutrophils and inflammatory cells, significantly decrease the levels of ALT, AST, ROS (P<0.01), pro-inflammatory cytokines and neutrophil chemokines (P<0.05) in the liver of LPS model mice, and decrease protein expressions of TLR4 and NF-κB p65 in inflammatory pathway (P<0.001). Conclusion: The MVE could improve the liver injury of mice with acute inflammation induced by LPS, and exert its hepatoprotective mechanism by regulating the balance of pro-inflammatory and anti-inflammatory factors and inhibiting TLR4-NF-κB signal pathway.