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黄精多糖提取、分离纯化及生物活性研究进展

杨茂会 周欣 谯政文 赵超 龚小见 邓青芳 陈华国

杨茂会,周欣,谯政文,等. 黄精多糖提取、分离纯化及生物活性研究进展[J]. 食品工业科技,2022,43(12):407−416. doi:  10.13386/j.issn1002-0306.2021060142
引用本文: 杨茂会,周欣,谯政文,等. 黄精多糖提取、分离纯化及生物活性研究进展[J]. 食品工业科技,2022,43(12):407−416. doi:  10.13386/j.issn1002-0306.2021060142
YANG Maohui, ZHOU Xin, QIAO Zhengwen, et al. Recent Advances in Polygonatum Polysaccharides: Extraction, Isolation, Purification and Bioactivities[J]. Science and Technology of Food Industry, 2022, 43(12): 407−416. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2021060142
Citation: YANG Maohui, ZHOU Xin, QIAO Zhengwen, et al. Recent Advances in Polygonatum Polysaccharides: Extraction, Isolation, Purification and Bioactivities[J]. Science and Technology of Food Industry, 2022, 43(12): 407−416. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2021060142

黄精多糖提取、分离纯化及生物活性研究进展

doi: 10.13386/j.issn1002-0306.2021060142
基金项目: 贵州省高层次创新人才培养项目(黔科合人才[2015]4033号);贵州师范大学培育项目(黔科合平台人才[2018]5769)。
详细信息
    作者简介:

    杨茂会(1996−),男,硕士研究生,研究方向:药用植物开发与利用,E-mail:1651102239@qq.com

    通讯作者:

    陈华国(1981−),男,博士,教授,研究方向:中药质量控制、药物体内代谢以及药品、保健食品开发,E-mail:chenhuaguo1981@163.com

  • 中图分类号: TS201.1

Recent Advances in Polygonatum Polysaccharides: Extraction, Isolation, Purification and Bioactivities

  • 摘要: 黄精属于百合科黄精属的草本植物,具有补气养阴,健脾,润肺,益肾等功效。多糖是黄精主要的活性成分之一,且含量丰富。黄精多糖作为一种药食两用的天然产物资源,其复杂的结构和丰富的生物活性成为食品、医药等领域开发和研究的热点之一。本文主要从黄精多糖的提取、分离纯化、结构分析、生物活性等方面的研究进行综述,并对黄精多糖的研究方向与应用前景进行展望,以期为进一步开发利用黄精多糖提供理论参考。
  • 表  1  黄精多糖提取方法与结果

    Table  1.   Methods and results of extraction of polysaccharides from Polygonatum

    提取方法黄精种类提取条件粗多糖提取率(%)参考文献
    水提取法黄精料液比:1:20,提取温度:85 ℃,提取时间:2 h12.50[14]
    滇黄精料液比:1:20,提取温度:80 ℃,提取时间:2 h13.34[15]
    碱提取法黄精料液比:1:15,粒度:60目,碱液:3%NaOH溶液11.89[16]
    超高压提取法黄精料液比:1:17,粒径:40目,压力:255 MPa,提取温度:常温,保压时间9.5 min,提取溶剂:水25.01[17]
    减压提取法黄精液料比:1:14,提取温度75 ℃,提取时间8 min,提取次数:2次,提取压力−0.074 MPa20.00[18]
    闪式提取法黄精料液比:1:18,提取时间:40 s,提取次数:1次,提取电压:50 V14.99[19]
    微波提取法黄精料液比:1:50,浸泡时间:60 min,微波功率:450 W,提取时间:5 min11.82[20]
    超声提取法滇黄精料液比:1:20,提取温度:80 ℃,提取时间:60 min,提取次数:3次14.09[21]
    多花黄精料液比:1:47.6,提取温度:63.8 ℃、提取时间:36.9 min、超声功率:225 W24.61[23]
    单一酶解法黄精料液比:1:22.4,纤维素酶酶添加量:3%,酶解pH:4.6,酶解温度:54.8 ℃,酶解时间:99 min11.22[24]
    复合酶解法黄精料液比:1:20,纤维素酶与木瓜蛋白酶质量比3:7,pH:5.0,酶解温度:
    50 ℃,酶量:5%,酶解时间:2 h
    22.00[11]
    黄精料液比:1:20,酶解温度 50 ℃,纤维素酶、木瓜蛋白酶加入0.45、
    1.05 g/dL,pH 5.0,酶解2 h后,浸提2 h
    21.55[25]
    超声波辅助酶法黄精料液比:1:30,复合酶添加量:6%,酶解温度:65 ℃,酶解时间:55 min25.63[26]
    超声-微波提取法黄精超声功率:50 W,超声频率:40 kHz,料液比:1:32,微波功率:300 W,提取时间:80 s11.19[27]
    下载: 导出CSV

    表  2  黄精多糖的结构特征

    Table  2.   Structural characteristics of Polygonatum polysaccharides

    黄精种类组分相对分子质量(kDa)单糖组成物质的量比糖苷键构型参考文献
    黄精PSP14.415Man:Glu:Gal14.96:2.13:82.91β-Glcp残基构型[6]
    黄精PSP22.236Rha:Glu:Gal:Xyl20.54:2.06:74.37:3.03β-Rhap残基构型[6]
    黄精PSP37.743Gal:Rha:Man:Glu:Xyl1.38:57.69:2.02:37.17:1.74β-Gal残基构型[6]
    黄精PSP46.467Gal:Rha:Man:Xyl2.00:72.63:20.74:4.63β-Gal残基构型[6]
    黄精P-1Glc:Gal:Ara:Xyl:Rhaβ-吡喃糖苷键[22]
    黄精P-2,3,4Glc:Gal:Ara:Xyl:Rha:Glucβ-吡喃糖苷键[22]
    黄精PSP8.912Fru:Glc8.7:1β-Fruc,Glcp残基构型[34]
    滇黄精PKPs-114.05Glc:Man:GalA:Gal:GluA:Ara7.22:1:0.16:0.11:0.05:0.02β-1,2-Glc糖苷键[35]
    多花黄精PCP12.09Ara:Gal:Glu:Man:GluA:GalA2.1:24:20.7:33.5:0.5:19.3β-糖残基型为主,
    含有少量α-糖残基型
    [35]
    多花黄精PCP342.6Ara:Gal:Glu:Man:Xyl:GluA:GalA22.2:58.7:3.9:4.9:0.5:8.5:1.5异头碳构型为α[35]
    注:Glc,葡萄糖;Gal,半乳糖;Ara,阿拉伯糖;Man,甘露糖;Fru,果糖;Rha,鼠李糖;Xyl,木糖;Gluc,葡萄糖醛酸;GalA,半乳糖醛酸;Glcp,吡喃葡萄糖;Rhap,吡喃鼠李糖;Fruc,吡喃果糖:−表示无相关数据。
    下载: 导出CSV

    表  3  黄精多糖的其他生物活性

    Table  3.   Other biological activities of Polygonatum polysaccharides

    生物活性实验模型剂量作用参考文献
    抑菌作用体外培养基模型2、4、6、8、10、12、14 mg/mL抑制枯草芽孢杆菌、金黄色葡萄球菌的活性[9]
    抗病毒猴肾衍生细胞模型10 mg/mL抑制疱疹病毒的活性,具有较好的抗病毒活性[62]
    抗衰老AD转基因小鼠模型40 mg/kg提高组织细胞端粒酶活性,达到延缓细胞衰老和凋亡的效应[63]
    衰老小鼠模型100、200、400 mg/kg增强呼吸链酶的活性,改善线粒体能量代谢,起到延缓衰老的作用[64]
    保护神经系统脑缺血大鼠模型750 mg/kg改善神经元结构,降低前额皮质和海马区Aβ1-42的蛋白表达[65]
    慢性脑缺血大鼠模型减轻神经细胞结构紊乱,降低前额皮质和海马区PS-1蛋白表达[66]
    调节心肌活性力竭小鼠模型500、1000 mg/kg抑制心肌组织中Ca2+-ATP 酶活性,维持细胞膜内外Ca2+的分布平衡[67]
    注1:−表示无相关数据。
    下载: 导出CSV
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  • 收稿日期:  2021-06-18
  • 网络出版日期:  2022-06-08
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