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食用槟榔废弃籽的活性成分提取及抗氧化、α-葡萄糖苷酶抑制活性分析

张培月 唐敏敏 宋菲 刘璨 陈华

张培月,唐敏敏,宋菲,等. 食用槟榔废弃籽的活性成分提取及抗氧化、α-葡萄糖苷酶抑制活性分析[J]. 食品工业科技,2022,43(12):253−260. doi:  10.13386/j.issn1002-0306.2021090023
引用本文: 张培月,唐敏敏,宋菲,等. 食用槟榔废弃籽的活性成分提取及抗氧化、α-葡萄糖苷酶抑制活性分析[J]. 食品工业科技,2022,43(12):253−260. doi:  10.13386/j.issn1002-0306.2021090023
ZHANG Peiyue, TANG Minmin, SONG Fei, et al. Bioactive Composition, Antioxidant, and α-Glucosidase Inhibition of Edible Areca catechu Waste Seed[J]. Science and Technology of Food Industry, 2022, 43(12): 253−260. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2021090023
Citation: ZHANG Peiyue, TANG Minmin, SONG Fei, et al. Bioactive Composition, Antioxidant, and α-Glucosidase Inhibition of Edible Areca catechu Waste Seed[J]. Science and Technology of Food Industry, 2022, 43(12): 253−260. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2021090023

食用槟榔废弃籽的活性成分提取及抗氧化、α-葡萄糖苷酶抑制活性分析

doi: 10.13386/j.issn1002-0306.2021090023
基金项目: 海南省自然科学基金高层次人才项目:辐照处理对槟榔鲜果品质影响规律的研究(320RC737);槟榔产业创新团队:槟榔产品加工工艺研究及新产品研发(1630152017017)。
详细信息
    作者简介:

    张培月(1995−),男,硕士研究生,研究方向:食品加工与安全,E-mail:865007082@qq.com

    通讯作者:

    陈华(1971−),男,硕士,副研究员,研究方向:食品加工与安全,E-mail:ch111666@126.com

  • 中图分类号: TS201.2

Bioactive Composition, Antioxidant, and α-Glucosidase Inhibition of Edible Areca catechu Waste Seed

  • 摘要: 针对食用槟榔加工后槟榔籽被当作废弃物而基本无大规模利用的问题,本研究以食用槟榔加工后的废弃籽为研究对象,75%的乙醇溶液为提取溶剂,超声辅助提取,得到加工后槟榔籽75%乙醇提取物(processed seed extraction,PSE),测定其总多酚、总黄酮和原花青素含量。采用DPPH法、ABTS+法和Fe3+还原法3种测定方法对槟榔提取物体外抗氧化活性进行评价。本文还研究了PSE对α-葡萄糖苷酶活力的抑制能力。结果表明,PSE含有丰富的多酚和黄酮等活性物质,其中,总多酚含量为(127.29±5.16)mg 没食子酸/g提取物、总黄酮含量为(421.84±13.82) mg 芦丁/g提取物,原花青素含量为(87.83±6.60) mg儿茶素/g提取物。PSE检测到44种黄酮类化合物,其中儿茶素相对含量高达77.27%。PSE具有一定的自由基清除力,清除DPPH自由基和ABTS+自由基的IC50值分别为(310.10±0.62)和(150.10±11.57) μg/mL。另外,PSE表现出较强的α-葡萄糖苷酶抑制能力,其对α-葡萄糖苷酶活力的抑制能力(IC50值为(90.10±1.89) μg/mL)显著(P<0.05)强于阳性对照阿卡波糖(IC50值为(453.60±4.02) μg/mL)。因此,回收再利用食用槟榔加工后的槟榔籽非常有必要。
  • 图  1  PSE和VC对DPPH自由基的清除能力

    Figure  1.  Ability to scavenge DPPH radicals of PSE and VC

    图  2  PSE和VC对ABTS+自由基的清除能力

    Figure  2.  Ability to scavenge ABTS+ radicals of PSE and VC

    图  3  PSE和VC的还原能力

    Figure  3.  Reducing power of PSE and VC

    图  4  PSE和阿卡波糖对α-葡萄糖苷酶活性的抑制作用

    Figure  4.  Inhibition of PSE and acarbose on α-glucosidase activity

    表  1  α-葡萄糖苷酶活力抑制试验反应体系

    Table  1.   Experimental reaction system of α-glucoside enzyme vitality inhibition experimental reaction system

    组别酶溶液
    (mL)
    抑制剂
    (mL)
    PBS
    (mL)
    pNPG
    (mL)
    Na2CO3
    (mL)
    样品管0.40.400.20.5
    样品对照管00.40.40.20.5
    对照管0.400.40.20.5
    空白对照管000.80.20.5
    下载: 导出CSV

    表  2  槟榔废弃籽中总多酚、总黄酮和原花青素的含量

    Table  2.   Total polyphenols, total flavonoids and progesterone content in processed betel nut seeds

    指标总多酚(mg没食子酸/g提取物)总黄酮(mg芦丁/g提取物)原花青素(mg儿茶素/g提取物)
    含量127.29±5.16421.84±13.8287.83±6.60
    下载: 导出CSV

    表  3  PSE中的类黄酮组分相对含量

    Table  3.   Relative contents of flavonoids in PSE

    序号化合物名称分子式保留时间(min)质荷m/z相对含量(%)
    1Cianidanol儿茶素C15H14O63.64290.9477.27
    2L-Epicatechin表儿茶素C15H14O64.49291.38.53
    3Procyanidin B1原花青素B1C30H26O122.95579.54.66
    4Procyanidin B2原花青素B2C30H26O124.03578.9923.45
    5Rutin芦丁C27H30O165.68610.9741.10
    6Ononin刺芒柄花苷C22H22O98.19431.40.71
    7Tangeretin桔皮素C20H20O714.38372.7810.64
    8Isorhamnetin异鼠李素C16H12O710.58317.0850.45
    9Liquiritin甘草苷C21H22O96.06416.9840.43
    10Chalconaringenin柑橘查耳酮C15H12O510.14272.9130.23
    11Nobiletin川陈皮素C21H22O813.18403.40.23
    12Quercetin槲皮素C15H10O78.88300.890.20
    13Diosmetin香叶木素C16H12O610.37301.30.20
    14Apigenin-7-glucoside
    芹甙元-7-葡萄糖苷
    C21H20O106.98432.9670.20
    15Naringenin柚皮素C15H12O510.11273.30.19
    16Pinocembrin松属素C15H12O413.62257.30.18
    17Taxifolin花旗松素C15H12O76.31304.8020.13
    18Genkwanin芫花素C16H12O513.58284.9430.11
    19Eriocitrin圣草次苷C27H32O155.85595.0030.11
    20Isoliquiritigenin异甘草素C15H12O411.29256.9270.11
    21总计99.11
    下载: 导出CSV
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出版历程
  • 收稿日期:  2021-09-03
  • 网络出版日期:  2022-05-14
  • 刊出日期:  2022-06-08

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