食用槟榔废弃籽的活性成分提取及抗氧化、<i>α</i>-葡萄糖苷酶抑制活性分析

张培月; 唐敏敏; 宋菲; 刘璨; 陈华

doi:10.13386/j.issn1002-0306.2021090023

食用槟榔废弃籽的活性成分提取及抗氧化、α-葡萄糖苷酶抑制活性分析

doi: 10.13386/j.issn1002-0306.2021090023

张培月^{1, 2,},
唐敏敏^{2, 3},
宋菲^{2, 3},
刘璨^{1, 2},
陈华^{1, 2, 3, ,}

1.
华中农业大学食品科学技术学院，湖北武汉 430070
2.
中国热带农业科学院椰子研究所，海南文昌 571339
3.
海南省槟榔产业工程研究中心，海南文昌 571339

基金项目: 海南省自然科学基金高层次人才项目：辐照处理对槟榔鲜果品质影响规律的研究（320RC737）；槟榔产业创新团队：槟榔产品加工工艺研究及新产品研发（1630152017017）。

详细信息

作者简介:
张培月（1995−），男，硕士研究生，研究方向：食品加工与安全，E-mail：865007082@qq.com

通讯作者:
陈华（1971−），男，硕士，副研究员，研究方向：食品加工与安全，E-mail：ch111666@126.com

中图分类号: TS201.2
计量
- 文章访问数: 43
- HTML全文浏览量: 21
- PDF下载量: 11
- 被引次数: 0
出版历程
- 收稿日期: 2021-09-03
- 网络出版日期: 2022-05-14
- 刊出日期: 2022-06-08

Bioactive Composition, Antioxidant, and α-Glucosidase Inhibition of Edible Areca catechu Waste Seed

ZHANG Peiyue^{1, 2
,},
TANG Minmin^{2, 3},
SONG Fei^{2, 3},
LIU Can^{1, 2},
CHEN Hua^{1, 2, 3
, ,}

1.
College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
2.
Coconut Research Institute of Chinese Academy of Tropical Agricultural Sciences, Wenchang 571339, China
3.
Hainan Engineering Research Center of Arecanut Industry, Wenchang 571339, China

摘要

摘要: 针对食用槟榔加工后槟榔籽被当作废弃物而基本无大规模利用的问题，本研究以食用槟榔加工后的废弃籽为研究对象，75%的乙醇溶液为提取溶剂，超声辅助提取，得到加工后槟榔籽75%乙醇提取物（processed seed extraction，PSE），测定其总多酚、总黄酮和原花青素含量。采用DPPH法、ABTS⁺法和Fe³⁺还原法3种测定方法对槟榔提取物体外抗氧化活性进行评价。本文还研究了PSE对α-葡萄糖苷酶活力的抑制能力。结果表明，PSE含有丰富的多酚和黄酮等活性物质，其中，总多酚含量为（127.29±5.16）mg 没食子酸/g提取物、总黄酮含量为（421.84±13.82） mg 芦丁/g提取物，原花青素含量为（87.83±6.60） mg儿茶素/g提取物。PSE检测到44种黄酮类化合物，其中儿茶素相对含量高达77.27%。PSE具有一定的自由基清除力，清除DPPH自由基和ABTS⁺自由基的IC₅₀值分别为（310.10±0.62）和（150.10±11.57） μg/mL。另外，PSE表现出较强的α-葡萄糖苷酶抑制能力，其对α-葡萄糖苷酶活力的抑制能力（IC₅₀值为（90.10±1.89） μg/mL）显著（P<0.05）强于阳性对照阿卡波糖（IC₅₀值为（453.60±4.02） μg/mL）。因此，回收再利用食用槟榔加工后的槟榔籽非常有必要。
- 食用槟榔 /
- 废弃籽 /
- 总多酚 /
- 总黄酮 /
- 原花青素 /
- 抗氧化活性 /
- α-葡萄糖苷酶
Abstract: In view of the problem that edible Areca catechu seeds treated as waste and cannot be massively used after processing, in this study, the waste seeds after edible Areca catechu processing (PAS) are used. 75% ethanol extract of PAS (processed seed extraction, PSE) was extracted assisted by ultrasonic with a 75% ethanol solution as the extraction solvent. The contents of total polyphenols, total flavonoids and proanthocyanidins in the PSE were determined. Three methods (radical scavenging activities and Fe³⁺ reduction) were used to evaluate the antioxidant activity of PSE. Then the inhibitory of PSE on the activity of α-glucosidase was studied. The result showed that PSE was rich in polyphenols and flavonoid. The total contents of polyphenols, flavonoids and proanthocyanidins were (127.29±5.16) milligram gallic acid, (421.84±13.82) milligram rutin, and (87.83±6.60) milligram epicatechin in per gram of PSE, respectively. PSE contains 44 flavonoids were detected in PSE, in which the relative content of catechins was up to 77.27%. The IC₅₀ for scavenging DPPH free radical and ABTS⁺ free radical of PSE were (310.10±0.62) and (150.10±11.57) μg/mL, respectively. In addition, PSE had significant inhibitory effect on α-glucosidase, and its ability to inhibit α-glucosidase (IC₅₀=(90.10±1.89) μg/mL) was significantly stronger (P<0.05) than acabosose as a positive control (IC₅₀=(453.60±4.02) μg/mL). Therefore, it is necessary to recycle the PAS.
- adible Areca catechu /
- waste seed /
- total polyphenols /
- total flavonoids /
- proanthocyanidins /
- antioxidant activity /
- α-glucosidase

HTML全文

图 1 PSE和V_C对DPPH自由基的清除能力

Figure 1. Ability to scavenge DPPH radicals of PSE and V_C

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图 2 PSE和V_C对ABTS⁺自由基的清除能力

Figure 2. Ability to scavenge ABTS⁺ radicals of PSE and V_C

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图 3 PSE和V_C的还原能力

Figure 3. Reducing power of PSE and V_C

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图 4 PSE和阿卡波糖对α-葡萄糖苷酶活性的抑制作用

Figure 4. Inhibition of PSE and acarbose on α-glucosidase activity

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表 1 α-葡萄糖苷酶活力抑制试验反应体系

Table 1. Experimental reaction system of α-glucoside enzyme vitality inhibition experimental reaction system

组别	酶溶液（mL）	抑制剂（mL）	PBS （mL）	pNPG （mL）	Na₂CO₃ （mL）
样品管	0.4	0.4	0	0.2	0.5
样品对照管	0	0.4	0.4	0.2	0.5
对照管	0.4	0	0.4	0.2	0.5
空白对照管	0	0	0.8	0.2	0.5

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表 2 槟榔废弃籽中总多酚、总黄酮和原花青素的含量

Table 2. Total polyphenols, total flavonoids and progesterone content in processed betel nut seeds

指标	总多酚（mg没食子酸/g提取物）	总黄酮（mg芦丁/g提取物）	原花青素（mg儿茶素/g提取物）
含量	127.29±5.16	421.84±13.82	87.83±6.60

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表 3 PSE中的类黄酮组分相对含量

Table 3. Relative contents of flavonoids in PSE

序号	化合物名称	分子式	保留时间（min）	质荷m/z	相对含量（%）
1	Cianidanol儿茶素	C₁₅H₁₄O₆	3.64	290.94	77.27
2	L-Epicatechin表儿茶素	C₁₅H₁₄O₆	4.49	291.3	8.53
3	Procyanidin B1原花青素B1	C₃₀H₂₆O₁₂	2.95	579.5	4.66
4	Procyanidin B2原花青素B2	C₃₀H₂₆O₁₂	4.03	578.992	3.45
5	Rutin芦丁	C₂₇H₃₀O₁₆	5.68	610.974	1.10
6	Ononin刺芒柄花苷	C₂₂H₂₂O₉	8.19	431.4	0.71
7	Tangeretin桔皮素	C₂₀H₂₀O₇	14.38	372.781	0.64
8	Isorhamnetin异鼠李素	C₁₆H₁₂O₇	10.58	317.085	0.45
9	Liquiritin甘草苷	C₂₁H₂₂O₉	6.06	416.984	0.43
10	Chalconaringenin柑橘查耳酮	C₁₅H₁₂O₅	10.14	272.913	0.23
11	Nobiletin川陈皮素	C₂₁H₂₂O₈	13.18	403.4	0.23
12	Quercetin槲皮素	C₁₅H₁₀O₇	8.88	300.89	0.20
13	Diosmetin香叶木素	C₁₆H₁₂O₆	10.37	301.3	0.20
14	Apigenin-7-glucoside 芹甙元-7-葡萄糖苷	C₂₁H₂₀O₁₀	6.98	432.967	0.20
15	Naringenin柚皮素	C₁₅H₁₂O₅	10.11	273.3	0.19
16	Pinocembrin松属素	C₁₅H₁₂O₄	13.62	257.3	0.18
17	Taxifolin花旗松素	C₁₅H₁₂O₇	6.31	304.802	0.13
18	Genkwanin芫花素	C₁₆H₁₂O₅	13.58	284.943	0.11
19	Eriocitrin圣草次苷	C₂₇H₃₂O₁₅	5.85	595.003	0.11
20	Isoliquiritigenin异甘草素	C₁₅H₁₂O₄	11.29	256.927	0.11
21	总计				99.11

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