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热预处理和氧化预处理对鼠李糖乳杆菌喷雾干燥菌粉性质的影响

张臣臣 桂亚 韩越眉 瓦云超 印伯星 顾瑞霞

张臣臣,桂亚,韩越眉,等. 热预处理和氧化预处理对鼠李糖乳杆菌喷雾干燥菌粉性质的影响[J]. 食品工业科技,2022,43(12):132−137. doi:  10.13386/j.issn1002-0306.2021090329
引用本文: 张臣臣,桂亚,韩越眉,等. 热预处理和氧化预处理对鼠李糖乳杆菌喷雾干燥菌粉性质的影响[J]. 食品工业科技,2022,43(12):132−137. doi:  10.13386/j.issn1002-0306.2021090329
ZHANG Chenchen, GUI Ya, HAN Yuemei, et al. Influence of Heat Pre-treatment and Oxidative Pre-treatment on the Spray Dried Powder of Lactobacillus rhamnosus[J]. Science and Technology of Food Industry, 2022, 43(12): 132−137. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2021090329
Citation: ZHANG Chenchen, GUI Ya, HAN Yuemei, et al. Influence of Heat Pre-treatment and Oxidative Pre-treatment on the Spray Dried Powder of Lactobacillus rhamnosus[J]. Science and Technology of Food Industry, 2022, 43(12): 132−137. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2021090329

热预处理和氧化预处理对鼠李糖乳杆菌喷雾干燥菌粉性质的影响

doi: 10.13386/j.issn1002-0306.2021090329
基金项目: 国家自然科学基金青年科学基金项目(31801565);江苏省自然科学基金青年基金项目(BK20180910);扬州市-扬州大学市校合作共建科技创新平台(YZ2020265);2019年度扬州市“绿扬金凤计划”。
详细信息
    作者简介:

    张臣臣(1988−),男,博士,副教授,研究方向:乳品微生物,E-mail:cczhang@yzu.edu.cn

    通讯作者:

    顾瑞霞(1963−),男,博士,教授,研究方向:乳品工程,E-mail:guruixia1963@163.com

  • 中图分类号: TS252.1

Influence of Heat Pre-treatment and Oxidative Pre-treatment on the Spray Dried Powder of Lactobacillus rhamnosus

  • 摘要: 喷雾干燥法是低成本、高效率的益生菌菌粉生产方法,但是容易导致严重的活性损失。本研究通过热预处理和氧化预处理提高鼠李糖乳杆菌hsryfm 1301喷雾干燥菌粉的存活率、胃肠道耐受能力和耐贮藏能力。以未预处理菌体为对照,采用46 ℃ 1 h的热预处理条件或0.5 mmol/L H2O2 1 h的氧化预处理条件处理菌体后进行喷雾干燥。结果显示,对照组喷雾干燥存活率仅为29%,而热预处理组和氧化预处理组喷雾干燥的存活率为98%和76%,分别提高到3.38和2.62倍。对照组菌粉的人工胃液耐受存活率为0.8%,而热预处理组和氧化预处理组菌粉的人工胃液存活率达到了76%和72%,分别提高到了原来的95.0和92.5倍。此外,热预处理组菌粉在−20 ℃下贮藏4个月,菌体存活率仍大于80%,氧化预处理组菌粉贮藏4个月的存活率则保持高于40%。本研究表明,鼠李糖乳杆菌hsryfm 1301经过热预处理或氧化预处理后,菌体获得的热胁迫和氧化胁迫耐受能力,有助于其在喷雾干燥中存活。
  • 图  1  热预处理和氧化预处理后菌株的喷雾干燥存活率

    Figure  1.  Survival rates of heat pre-treated or oxidative pre-treated L. rhamnosus hsryfm 1301 cells during spray drying

    注:不同小写字母表示差异显著,P<0.05;图2同。

    图  2  热预处理和氧化预处理喷雾干燥菌粉的胃肠道环境耐受能力

    Figure  2.  Resistance of spray dried powder to simulated gastrointestinal digestion

    图  3  热预处理和氧化预处理喷雾干燥菌粉在不同贮藏条件下的存活能力

    Figure  3.  Survival rates of spray dried powder during storage

    注: a:−20 ℃;b:4 ℃;c:室温。

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出版历程
  • 收稿日期:  2021-09-28
  • 网络出版日期:  2022-04-27
  • 刊出日期:  2022-06-08

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