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中国精品科技期刊2020
张孟琴,徐路,张俊波,等. 三叶木通果皮主要营养成分、活性成分含量测定及果皮提取物抗氧化活性评价[J]. 食品工业科技,2022,43(10):388−394. doi: 10.13386/j.issn1002-0306.2021090348.
引用本文: 张孟琴,徐路,张俊波,等. 三叶木通果皮主要营养成分、活性成分含量测定及果皮提取物抗氧化活性评价[J]. 食品工业科技,2022,43(10):388−394. doi: 10.13386/j.issn1002-0306.2021090348.
ZHANG Mengqin, XU Lu, ZHANG Junbo, et al. Determination of Contents of the Main Nutritional Components, Functional Components of Akebia trifoliata Pericarp and the Antioxidant Activity of Its Extracts[J]. Science and Technology of Food Industry, 2022, 43(10): 388−394. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021090348.
Citation: ZHANG Mengqin, XU Lu, ZHANG Junbo, et al. Determination of Contents of the Main Nutritional Components, Functional Components of Akebia trifoliata Pericarp and the Antioxidant Activity of Its Extracts[J]. Science and Technology of Food Industry, 2022, 43(10): 388−394. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021090348.

三叶木通果皮主要营养成分、活性成分含量测定及果皮提取物抗氧化活性评价

Determination of Contents of the Main Nutritional Components, Functional Components of Akebia trifoliata Pericarp and the Antioxidant Activity of Its Extracts

  • 摘要: 为了探究三叶木通果皮的营养价值及活性功能,本研究采用凯氏定氮法、酸水解法、苯酚硫酸法、3,5-二硝基水杨酸法、ICP-AES、亚硝酸钠-三氯化铝法、福林酚法和香草醛-高氯酸等方法,测定了三叶木通果皮中的蛋白质、脂肪、总糖、还原糖、8种矿质元素及粗纤维素等主要营养成分与总黄酮、总酚及总皂苷等活性物质的含量,并研究了果皮提取物的抗氧化活性。结果表明,三叶木通果皮中蛋白质(8.16%±0.11%)、还原糖(19.31%±0.21%)、总糖(32.61%±0.18%)等主要营养成分的含量均较高,其中,果胶含量达到(20.08%±0.20%);所测8种营养元素中,K含量最高(14061.17 µg/g DW),其次为Ca(4959.28 µg/g DW),8种元素含量高低顺序为K>Ca>Mg>Fe>Mn>Na>Cu>Zn。果皮中总黄酮、总酚及总皂苷等活性物质的含量分别为(20.58±0.12)、(31.53±0.18)、(45.20±12.04)mg/g DW;三叶木通果皮提取物清除DPPH自由基、ABTS+自由基的IC50分别为0.049、0.052 mg/mL ,且清除能力与果皮提取物的质量浓度呈现出良好的量效关系。果皮中总黄酮、总酚、总皂苷的含量与抗氧化活性均呈现极显著正相关(P<0.01)。综上,三叶木通果皮是一种营养价值较高、具有一定抗氧化活性的功能型新材料。

     

    Abstract: In order to explore the nutritional value and active function of Akebia trifoliata pericarp, the main nutritional components contents of protein, fat, total sugar, reducing sugar, 8 mineral elements, total flavonoids, total phenols and total saponins were determined by using Kjeldahl method, acid hydrolysis, phenol sulfuric acid method, 3,5-dinitrosalicylic acid method, ICP-AES method, NaNO2-AlCl3 method, Folin-ciocalten and vanillin-perchloric acid. The antioxidant capacity of pericarp extracts was also evaluated. The results showed that the contents of protein (8.16%±0.11%), reducing sugar (19.31%±0.21%) and total sugar (32.61%±0.18%) in the peel of Akebia trifoliata were high, and the pectin content determined by gravimetric method was (20.08%±0.20%). Among the eight nutrient elements measured, K content was the richest (14061.17 μg/g DW), followed by Ca (4959.28 μg/g DW). The orders of the contents of eight mineral elements were K>Ca>Mg>Fe>Mn>Na>Cu>Zn. The contents of total flavonoids, polyphenolics and saponins were (20.58±0.12), (31.53±0.18) and (45.20±12.04) mg/g DW, respectively. The scavenging capabilities of extraction on the free radicals of DPPH and ABTS+ were also investigated. When the scavenging rate reached to 50%, the IC50 values on DPPH and ABTS+ free radicals scavenging were 0.049, 0.052 mg/mL, respectively. The scavenging ability of extracts in Akebia trifoliata pericarp was dose-dependent. The contents of total flavonoids, total polyphenols and total saponins had strong and positive correlations with antioxidant activity (P<0.01). In summary, Akebia trifoliata pericarp should be new resources with high nutritional values and strong antioxidant activities worth for in-depth development.

     

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