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刺梨果酒改善2型糖尿病大鼠糖脂代谢紊乱的研究

安玉红 周靖 朱德星

安玉红,周靖,朱德星. 刺梨果酒改善2型糖尿病大鼠糖脂代谢紊乱的研究[J]. 食品工业科技,2022,43(12):361−368. doi:  10.13386/j.issn1002-0306.2021100316
引用本文: 安玉红,周靖,朱德星. 刺梨果酒改善2型糖尿病大鼠糖脂代谢紊乱的研究[J]. 食品工业科技,2022,43(12):361−368. doi:  10.13386/j.issn1002-0306.2021100316
AN Yuhong, ZHOU Jing, ZHU Dexing. Rosa roxburghii Fruit Wine Improves Glucose and Lipid Metabolism Disorder in Type 2 Diabetic Rats[J]. Science and Technology of Food Industry, 2022, 43(12): 361−368. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2021100316
Citation: AN Yuhong, ZHOU Jing, ZHU Dexing. Rosa roxburghii Fruit Wine Improves Glucose and Lipid Metabolism Disorder in Type 2 Diabetic Rats[J]. Science and Technology of Food Industry, 2022, 43(12): 361−368. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2021100316

刺梨果酒改善2型糖尿病大鼠糖脂代谢紊乱的研究

doi: 10.13386/j.issn1002-0306.2021100316
基金项目: 毕节市科技项目(毕科联合字ZY[2017]01号);贵州省林业局科技项目(黔林科合[2020]2011)。
详细信息
    作者简介:

    安玉红(1982−),女,硕士,副教授,研究方向:食品科学,E-mail:304672741@qq.com

  • 中图分类号: R363

Rosa roxburghii Fruit Wine Improves Glucose and Lipid Metabolism Disorder in Type 2 Diabetic Rats

  • 摘要: 为探究刺梨果酒对2型糖尿病大鼠糖脂代谢紊乱的影响及可能机制。采用高脂高糖膳食联合腹腔注射链佐霉素(Streptozocin,STZ)建立2型糖尿病大鼠模型,将造模成功的大鼠分为刺梨果酒高(8 mL/kg)、中(4 mL/kg)、低(2 mL/kg)剂量组和模型组,并设空白对照组,给药期间每2周测一次空腹血糖,实验时间28 d。实验结束后测量血清和肝脏中高密度脂蛋白胆固醇(High-density lipoprotein cholesterol,HDL-C)、果糖胺(fructosamine,FMN)、甘油三酯(Triglyceride,TG)、低密度脂蛋白胆固醇(Low-density lipoprotein cholesterol,LDL-C)、总胆固醇(Total cholesterol,TC)、肝糖原等含量;采用实时灾光定量PCR(real time polymerase chain reaction,RT-PCR)测定肝脏中腺苷酸活化蛋白激酶(AMP-activated protein kinase α,AMPK)、乙酰辅酶A羧化酶(Acetyl-CoA carboxylases alpha,ACACA)、β-羟-β-甲戊二酸单酰辅酶A还原酶(3-hydroxy-3-methylglutaryl coenzyme A reductase,HMG-CoA)、脂肪酸合成酶(fatty acid synthetase,FASN)、和葡萄糖转运载体2(Glucose Transporter 2,GLUT2)、胆固醇7α-羟化酶(Cholesterol 7α-hydroxylase,CYP7A1)等mRNA相对表达量。结果表明,与模型组相比,刺梨果酒可减缓2型糖尿病大鼠体重减轻和多饮、多食的症状;高、中剂量刺梨果酒降低实验大鼠空腹血糖和果糖胺的效果显著(P<0.05);各剂量组均有降低实验大鼠血清和肝脏TC、TG、LDL-C的含量和升高HDL-C含量的作用,其中高、中剂量效果显著(P<0.05);低、中、高刺梨果酒均可显著(P<0.05)上调AMPK、GLUT2和ACACA mRNA表达量,低、中、高刺梨果酒均可上调FASN mRNA表达量,其中高、中剂量组上调FASN mRNA表达量显著(P<0.05);中、高剂量刺梨果酒可显著(P<0.05)下调G6Pase、PEPCK、HMG-COA和CYP7A1 mRNA表达量。结论:刺梨果酒改善2型糖尿病大鼠糖脂代谢紊乱的机制可能与通过抑制内源性胆固醇、增加脂肪的从头合成及提高葡萄糖跨膜转速率有关。
  • 图  1  刺梨果酒对大鼠空腹血糖(FBG)(A)和果糖胺(FMN)(B)的影响(n=10)

    Figure  1.  Effects of Rosa roxburghii fruit wine on fasting blood glucose (FBG) (A) and fructosamine (FMN) (B) in rats (n=10)

    图  2  刺梨果酒对大鼠血脂和肝脂的影响(n=10)

    Figure  2.  Effects of Rosa roxburghii fruit wine on blood lipid and liver lipid in rats (n=10)

    图  3  刺梨果酒对肝脏糖代谢关键基因mRNA水平的影响(n=10)

    Figure  3.  Effect of Rosa roxburghii fruit wine on mRNA level of key genes of liver glucose metabolism (n=10)

    图  4  刺梨果酒对对大鼠肝脏脂代谢关键基因mRNA水平的影响(n=10)

    Figure  4.  Effect of Rosa roxburghii fruit wine on mRNA level of key genes of lipid metabolism in rat liver (n=10)

    表  1  用于RT-PCR引物序列

    Table  1.   Primer sequences for RT-PCR

    基因名引物序列(5’~3’)
    上游下游
    HMG-COAGACCAACCTTCTACCTCAGCAAGACAACTCACCAGCCATCACAGT
    CYP7A1GAGGGATTGAAGCACAAGAACCATGCCCAGAGAATAGCGAGGT
    ACACACATCCGGCGACTTACGTTCAAACTTATCCCTTGCTCGGAA
    FASNTCAACCTGCTCCTGAAGCCGAAGCCTCAGAGCGACAATATCCAC
    AMPKCGGGGTCATTCTCTATGCTTTTTAAACCACTCGTGTTCCCT
    PEPCKGACAGACTCGCCCTATGTGGTGGGTTGCAGGCCCAGTTGTTG
    G6PaseGGCTCACTTTCCCCATCAGGTCCAAGTGCGAAACCAAACAGG
    GLUT2CCAGCACATACGACACCAGACGCCAAAGAACGAGGCGACCAT
    β-actinACGTCAGGTCATCACTATCGGGCATAGAGGTCTTTACGGATG
    下载: 导出CSV

    表  2  刺梨果酒对大鼠生长状况的影响(n=10)

    Table  2.   Effect of Rosa roxburghii fruit wine on growth of rats (n=10)

    组别初体重(g)末体重(g)体重增加量(g)采食量(g/4 w)饮水量(mL/4 w)
    Control207.92±8.54319.44±10.21111.52±5.05510.033±15.99528.21±4.21
    MC256.32±6.21*a275.69±12.62*b19.37±4.43*c862.83±10.93*a1299.48±10.32*a
    LD252.74±7.23a281.69±7.62b28.95±3.43b847.09±14.21a1178.90±12.91b
    MD257.33±5.32a296.35±5.61a39.02±5.31b819.32±18.32b928.19±14.21c
    HD253.08±8.77a291.07±8.16a37.99±5.83a792.99±12.61b891.19±17.21c
    注:不同字母a、b、c表示各试剂量与模型组之间存在显著性差异(P<0.05);*表示MC组与Control组间存在显著性差异(P<0.05)。Control:空白对照组;MC:模型对照组;LD:刺梨果酒低剂量组(2 mL/kg);MD:刺梨果酒中剂量组(4 mL/kg);HD:刺梨果酒高剂量组(8 mL/kg);图1~图4同。
    下载: 导出CSV
  • [1] GOLDENBERG R, PUNTHAKEE Z. Definition, classification and diagnosis of diabetes, prediabetes and metabolic syndrome[J]. Canadian Journal of Diabetes,2013,37(suppS1):S8−S11.
    [2] REN T, ZHU Y, KAN J. Zanthoxylum alkylamides activate phosphorylated AMPK and ameliorate glycolipid metabolism in the streptozotocin-induced diabetic rats[J]. Clinical and Experimental Hypertension (New York, NY:1993),2017,39(4):330−338. doi:  10.1080/10641963.2016.1259332
    [3] REN T, ZHU Y, XIA X, et al. Zanthoxylum alkylamides ameliorate protein metabolism disorder in STZ-induced diabetic rats[J]. Journal of Molecular Endocrinology,2017,58(3):113−125. doi:  10.1530/JME-16-0218
    [4] YOU Y M, REN T, ZHANG S Q, et al. Hypoglycemic effects of Zanthoxylum alkylamides by enhancing glucose metabolism and ameliorating pancreatic dysfunction in streptozotocin-induced diabetic rats[J]. Food & Function,2015,6(9):3144−3154.
    [5] DENNIS J M. Precision medicine in type 2 diabetes: Using individualized prediction models to optimize selection of treatment[J]. Diabetes,2020,69(10):dbi200002.
    [6] POUYA S, INGA P, PARASKEVI S, et al. Global and regional diabetes prevalence estimates for 2019 and projections for 2030 and 2045: Results from the international diabetes federation diabetes atlas, 9th edition[J]. Diabetes Research and Clinical Practice,2019,157:107843. doi:  10.1016/j.diabres.2019.107843
    [7] 中华医学会糖尿病学分会. 中国2型糖尿病防治指南(2020年版)[J]. 中华糖尿病杂志,2021,13(4):315−409. [Chinese Diabetes Society. Guideline for the prevention and treatment of type 2 diabetes mellitus in China (2020 edition)[J]. Chinese Journal of Diabetes Mellitus,2021,13(4):315−409.

    Chinese Diabetes Society. Guideline for the prevention and treatment of type 2 diabetes mellitus in China (2020 edition)[J]. Chinese Journal of Diabetes Mellitus, 2021, 13(4): 315-409.
    [8] LIY Z, FU C, WANG W, et al. Prevalence of chronic complications of type 2 diabetes mellitus in outpatients-a cross-sectional hospital based survey in urban China[J]. Health and Quality of Life Outcomes,2010,8(1):62. doi:  10.1186/1477-7525-8-62
    [9] KISA A, KISA S, COLLABORATORS G S. Global, regional, and national burden of stroke and its risk factors, 1990~2019: A systematic analysis for the global burden of disease study 2019[J]. The Lancet Neurology,2021:1−26.
    [10] YOSHIDA T, CHEN X M, HATANO T, et al. Tannins and related polyphenols of rosaceous medicinal plants. IV. Roxbins A and B from Rosa roxburghii fruits[J]. Chemical & Pharmaceutical Bulletin,1987,35(5):1817−1822.
    [11] 白静, 张宗泽, 鲁敏, 等. 贵州不同地区野生刺梨果实品质分析[J]. 贵州农业科学,2016,44(3):43−46. [BAI J, ZHANG Z Z, LU M, et al. Fruit quality of wild Rosa roxburghii germplasm resources from different regions in Guizhou[J]. Guizhou Agricultural Sciences,2016,44(3):43−46. doi:  10.3969/j.issn.1001-3601.2016.03.011

    BAI J, ZHANG Z Z, LU M, et al. Fruit quality of wild Rosa roxburghii germplasm resources from different regions in Guizhou[J]. Guizhou Agricultural Sciences, 2016, 44(3): 43-46. doi:  10.3969/j.issn.1001-3601.2016.03.011
    [12] 黄颖, 谭书明, 陈小敏, 等. 刺梨口服液对急性醉酒小鼠的解酒护肝作用[J]. 现代食品科技,2019,35(7):18−23. [HUANG Y, TAN S M, CHEN X M, et al. Antialcoholism effects of Rosa roxburghii Tratt oral liquid in acute drunkenness mice[J]. Modern Food Science and Technology,2019,35(7):18−23.

    HUANG Y, TAN S M, CHEN X M, et al. Antialcoholism effects of Rosa roxburghii Tratt oral liquid in acute drunkenness mice[J]. Modern Food Science and Technology, 2019, 35(7): 18-23.
    [13] 陈小敏, 谭书明, 黄颖, 等. 刺梨汁对Ⅰ型糖尿病小鼠的降糖作用[J]. 现代食品科技,2019,35(8):13−20. [CHEN X M, TAN S M, HUANG Y, et al. Hypoglycemic effect of Rosa roxburghii juice on type I diabetic mice[J]. Modern Food Science and Technology,2019,35(8):13−20.

    CHEN X M, TAN S M, HUANG Y, et al. Hypoglycemic effect of Rosa roxburghii juice on type I diabetic mice[J]. Modern Food Science and Technology, 2019, 35(8): 13-20.
    [14] 陈萍, 谭书明, 陈小敏, 等. 刺梨、蜂胶、山楂口服液的降血脂功能研究[J]. 现代食品科技,2019,35(8):78−83. [CHEN P, TAN S M, CHEN X M, et al. Study on hypolipidemic activity of Rosa roxburghii Tratt, propolis and crataegus oral liquid[J]. Modern Food Science and Technology,2019,35(8):78−83.

    CHEN P, TAN S M, CHEN X M, et al. Study on hypolipidemic activity of Rosa roxburghii Tratt, propolis and crataegus oral liquid[J]. Modern Food Science and Technology, 2019, 35(8): 78-83.
    [15] 陈超, 谭书明, 王画, 等. 刺梨及其活性成分对2型糖尿病小鼠糖脂代谢的影响[J/OL]. 食品科学: 1−14[2021-10-21]. http://kns.cnki.net/kcms/detail/11.2206.ts.20210723. 1740.028. htm.

    CHEN C, TAN S M, WANG H, et al. Effects of Rosa roxburghii Tratt and its active ingredients on glucose and lipid metabolism in type 2 diabetic mice[J/OL]. Food science: 1−14[2021-10-21]. http://kns.cnki.net/kcms/ detail/11.2206.ts.20210723. 1740.028. htm.
    [16] YU L M, FANG N, YANG X S, et al. Effects of Rosa roxburghii extract on proliferation and differentiation in human hepatoma SMMC-7721 cells and CD34(+) haematopoietic cells[J]. Journal of Health Scienc,2007,53(1):10−15. doi:  10.1248/jhs.53.10
    [17] 许立伟, 王炳宇, 杨馨悦, 等. 5种浆果果酒抗氧化活性差异及综合评价[J]. 中国酿造,2021,40(9):200−205. [XU L W, WANG B Y, YANG X Y, et al. Difference and comprehensive evaluation of antioxidant activity of 5 kinds of fruit wine[J]. China Brewing,2021,40(9):200−205. doi:  10.11882/j.issn.0254-5071.2021.09.036

    XU L W, WANG B Y, YANG X Y, et al. Difference and comprehensive evaluation of antioxidant activity of 5 kinds of fruit wine[J]. China Brewing, 2021, 40(9): 200-205. doi:  10.11882/j.issn.0254-5071.2021.09.036
    [18] RTSS A, ACTB B, ACPR B, et al. Physicochemical characterization, bioactive compounds, in vitro antioxidant activity, sensory profile and consumer acceptability of fermented alcoholic beverage obtained from Caatinga passion fruit (Passiflora cincinnata Mast. )[J]. LWT,2021,148:111714. doi:  10.1016/j.lwt.2021.111714
    [19] 安玉红, 陆敏涛, 卢秀, 等. 刺梨果酒通过胰岛素介导的PI3K途径改善1-型糖尿病大鼠机体糖代谢紊乱[J]. 现代食品科技,2020,36(7):25−33. [AN Y H, LU M T, LU X, et al. Rosa roxburghii Tratt wine can improve glucose metabolism disorder in type 1 diabetic rats through insulin-PI3K pathway[J]. Modern Food Science and Technology,2020,36(7):25−33.

    AN Y H, LU M T, LU X, et al. Rosa roxburghii Tratt wine can improve glucose metabolism disorder in type 1 diabetic rats through insulin-PI3K pathway[J]. Modern Food Science and Technology, 2020, 36(7): 25-33.
    [20] 陈珍, 陆敏涛, 徐方艳, 等. 刺梨果酒对高脂诱导肥胖小鼠脂代谢的影响[J/OL]. 食品工业科技: 1−16 [2021-10-21]. https: //doi. org/10.13386/j. issn1002-0306.2021050264.

    CHEN Z, LU M T, XU F Y, et al. Effects of Rosa roxburghii Tratt wine on lipid metabolism in hyperlipid-induced obesity mice[J/OL]. Science and Technology of Food Industry: 1−16 [2021-10-21]. https://doi.org/10.13386/j.issn1002-0306.2021050264.
    [21] 李劲松. 刺梨果酒的酿造方法: CN 105331481B[P]. 2018.

    LI J S. Brewing method of Rosa roxburghii Tratt wine: CN 105331481B[P]. 2018.
    [22] BEGORRE M A, DIB A, HABCHI K, et al. Microvascular vasodilator properties of the angiotensin II type 2 receptor in a mouse model of type 1 diabetes[J]. Scientific Reports,2017,7(1):45625. doi:  10.1038/srep45625
    [23] KIM H J, CHAE I G, LEE S G, et al. Effects of fermented red ginseng extracts on hyperglycemia in streptozotocin-induced diabetic rats[J]. Journal of Ginseng Research,2010,34(2):104−112. doi:  10.5142/jgr.2010.34.2.104
    [24] SARAVANAN R, KV BABU, V RAMACHANDRAN. Effect of rebaudioside A, a diterpenoid on glucose homeostasis in STZ-induced diabetic rats[J]. Journal of Physiology and Biochemistry,2012,68(3):421−431. doi:  10.1007/s13105-012-0156-0
    [25] RUSHFORTH N B, BENNETT P H, STEINBERG A G, et al. Diabetes in the pima indians. Evidence of bimodality in glucose tolerance distributions[J]. Diabetes,1971,20(11):756−758. doi:  10.2337/diab.20.11.756
    [26] H MALMSTRÖM, WALLDIUS G, GRILL V, et al. Fructosamine is a useful indicator of hyperglycaemia and glucose control in clinical and epidemiological studies–cross-sectional and longitudinal experience from the AMORIS cohort[J]. PloS One,2014,9(10):e111463. doi:  10.1371/journal.pone.0111463
    [27] WANG W, WANG C, DING X Q, et al. Quercetin and allopurinol reduce liver thioredoxin-interacting protein to alleviate inflammation and lipid accumulation in diabetic rats[J]. Brit J Pharmacol,2013,169(6):1352−1371. doi:  10.1111/bph.12226
    [28] 周艺. 刺梨茶对实验小鼠降血糖、血脂及抗氧化作用研究[D]. 贵州: 贵州大学, 2017.

    ZHOU Y. The study on the hypoglycemic and hypolipidemic and antioxidant activity effect from the tea of Rosa roxbugrhii Tratt[D]. Guizhou: Guizhou University, 2017.
    [29] 汪磊. 刺梨多糖的分离纯化、降血糖作用及其对肠道微生态的影响[D]. 广州: 华南理工大学, 2019.

    WANG L. Isolation, purification and hypoglycemic activity of polysaccharides from Rosa roxburghii Tratt fruit and their effect on gut microflora[D]. Guangzhou: South China University of Technology, 2019.
    [30] ZHANG Y, WANG X, WANG W, et al. Effects of grape seed proanthocyanins on high-fat and high-sugar diet-induced metabolic syndrome in rats[J]. Food Science,2020,41(1):112−120.
    [31] NGUYEN P, LERAY V, DIEZ M, et al. Liver lipid metabolism[J]. Journal of Animal Physiology & Animal Nutrition,2010,92(3):272−283.
    [32] 梁敏. 蓝靛果酒酿造工艺对花色苷及功能性质的影响[D]. 哈尔滨: 东北林业大学, 2018.

    LIANG M. Effect of brewing technology of blue honeysuckle wine on anthocyanins and functional properties[D]. Harbin: Northeast Forestry University, 2018.
    [33] 杜国慧, 刘博伟, 尹福在, 等. 利拉鲁肽对糖尿病小鼠肝糖异生关键酶PEPCK及G6pase的表达影响及机制探讨[J]. 中国比较医学杂志,2020,30(12):98−102. [DU G H, LIU B W, YIN F Z, et al. Mechanistic analysis of liraglutide effects on the expression levels of PEPCK and G6pase in the diabetic mouse liver[J]. Chinese Journal of Comparative Modicine,2020,30(12):98−102. doi:  10.3969/j.issn.1671-7856.2020.12.015

    DU G H, LIU B W, YIN F Z, et al. Mechanistic analysis of liraglutide effects on the expression levels of PEPCK and G6pase in the diabetic mouse liver[J]. Chinese Journal of Comparative Modicine, 2020, 30(12): 98-102. doi:  10.3969/j.issn.1671-7856.2020.12.015
    [34] LOCHHEAD P A, SALT I P, WALKER K S, et al. 5-aminoimidazole-4-carboxamide riboside mimics the effects of insulin on the expression of the 2 key gluconeogenic genes PEPCK and glucose-6-phosphatase[J]. Diabetes,2000,49(6):896−903. doi:  10.2337/diabetes.49.6.896
    [35] HE L, LI Y, ZENG N, et al. Regulation of basal expression of hepatic PEPCK and G6Pase by AKT2[J]. Biochemical Journal,2020,477(5):1−19.
    [36] DING L, HAN L, DUBE J, et al. WASH regulates glucose homeostasis by facilitating Glut2 receptor recycling in pancreatic β-cells[J]. Diabetes,2019,68(2):377−386. doi:  10.2337/db18-0189
    [37] ILBEIGI D, NOURBAKHSH M, PASALAR P, et al. Nicotinamide phosphoribosyltransferase knockdown leads to lipid accumulation in HepG2 cells through the SIRT1-AMPK pathway[J]. Cell Journal, 2020, 22(Suppl 1): 125-132.
    [38] KIM Y J, HOUNG S J, KIM J H, et al. Nanoemulsified green tea extract shows improved hypocholesterolemic effects in C57BL/6 mice[J]. Journal of Nutritional Biochemistry,2012,23(2):186−191. doi:  10.1016/j.jnutbio.2010.11.015
    [39] FESSLER, MICHAEL B. Regulation of adaptive immunity in health and disease by cholesterol metabolism[J]. Current Allergy & Asthma Reports,2015,15(8):42−48.
    [40] MITRO N, GODIO C, FABIANI E D, et al. Insights in the regulation of cholesterol 7alpha-hydroxylase gene reveal a target for modulating bile acid synthesis[J]. Hepatology,2010,46(3):885−897.
    [41] HANDSCHIN C, GNERRE. Species-specific mechanisms for cholesterol 7α-hydroxylase (CYP7A1) regulation by drugs and bile acids[J]. Archives of Biochemistry and Biophysics,2005,434(1):75−85. doi:  10.1016/j.abb.2004.10.010
    [42] SANDE T, SCHRIJVER E D, HEYNS W, et al. Role of the phosphatidylinositol 3’-Kinase/PTEN/Akt Kinase pathway in the overexpression of fatty acid synthase in LNCaP prostate cancer cells[J]. Cancer Research,2002,62(3):642−646.
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  • 收稿日期:  2021-11-01
  • 网络出版日期:  2022-04-26
  • 刊出日期:  2022-06-08

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