• EI
  • Scopus
  • 食品科学与工程领域高质量科技期刊分级目录第一方阵T1
  • DOAJ
  • EBSCO
  • 北大核心期刊
  • 中国核心学术期刊RCCSE
  • JST China
  • FSTA
  • 中国精品科技期刊
  • 中国农业核心期刊
  • CA
  • WJCI
  • 中国科技核心期刊CSTPCD
  • 中国生物医学SinoMed
中国精品科技期刊2020
师梦楠,彭云,张杰,等. 云南大叶种茶树花生化成分及体外抗氧化活性研究[J]. 食品工业科技,2022,43(16):298−306. doi: 10.13386/j.issn1002-0306.2021110135.
引用本文: 师梦楠,彭云,张杰,等. 云南大叶种茶树花生化成分及体外抗氧化活性研究[J]. 食品工业科技,2022,43(16):298−306. doi: 10.13386/j.issn1002-0306.2021110135.
SHI Mengnan, PENG Yun, ZHANG Jie, et al. Biochemical Components and in Vitro Antioxidant Activities of Large-leaf Species Tea Flowers in Yunnan[J]. Science and Technology of Food Industry, 2022, 43(16): 298−306. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021110135.
Citation: SHI Mengnan, PENG Yun, ZHANG Jie, et al. Biochemical Components and in Vitro Antioxidant Activities of Large-leaf Species Tea Flowers in Yunnan[J]. Science and Technology of Food Industry, 2022, 43(16): 298−306. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021110135.

云南大叶种茶树花生化成分及体外抗氧化活性研究

Biochemical Components and in Vitro Antioxidant Activities of Large-leaf Species Tea Flowers in Yunnan

  • 摘要: 本文对云南大叶种茶树花的生化成分和体外抗氧化活性进行了研究。实验对13个云南大叶种茶树花的主要生化指标与1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基清除能力、2,2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸(2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid),ABTS)自由基清除能力、总抗氧化能力(Total antioxidant capacity,TAC)、羟自由基(·OH)清除能力、超氧阴离子清除能力,5种体外抗氧化指标进行测定,主要生化成分和体外抗氧化指标进行了相关性分析。结果表明:茶树花水浸出物、茶多酚、氨基酸含量范围分别为41.22%~63.73%、7.74%~13.56%、1.61%~5.91%;咖啡碱、黄酮含量分别为4.98~8.46、4.21~8.63 mg/g,样品间生化含量及组成表现出一定的差异性。不同茶树花样品体外抗氧化能力存在显著性差异(P<0.05)。其中,地界古茶树花、布朗山古茶树花、冰岛古茶树花抗氧化活性表现良好,而秧塔大白茶古茶树花最弱。茶树花体外抗氧化活性与生化指标呈现相关性,茶多酚含量与总抗氧化能力极显著相关(P<0.01),表没食子儿茶素没食子酸酯(Epigallocatechin gallate,EGCG)、表儿茶素没食子酸酯(Epicatechin gallate,ECG)含量与总抗氧化能力显著相关(P<0.05),结果可作为预测茶树花抗氧化活性的重要指标。

     

    Abstract: In this paper, the chemical components and in vitro antioxidant activities of Camellia sinensis, a large-leaf species in Yunnan were studied. The main biochemical indicators of 13 Yunnan large-leaf tea tree flowers and 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging ability, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) free radical scavenging capacity, total antioxidant capacity (TAC), hydroxyl radical (·OH) scavenging capacity, superoxide anion scavenging capacity, five kinds of in vitro antioxidant indexes were measured. The correlation between the main biochemical components and in vitro antioxidant indexes was analyzed. The results showed that the contents of tea tree flower water extract, tea polyphenols and amino acids were 41.22%~63.73%, 7.74%~13.56%, 1.61%~5.91%, respectively. The contents of caffeine and flavonoids were 4.98~8.46, 4.21~8.63 mg/g, respectively. Each sample showed a certain difference. There were significant differences in the in vitro antioxidant capacity of different tea tree flower samples (P<0.05). Among them, the ancient tea tree flowers of Dijie, Bulang Mountain, and Bingdao showed good antioxidant activities, while the ancient tea flowers of Yangta white tea were the weakest. The antioxidant activity of tea tree flowers in vitro was correlated with biochemical indicators, tea polyphenols were significantly correlated with total antioxidant capacity (P<0.01), epigallocatechin gallate (EGCG), epicatechin gallate Epicatechin gallate (ECG) was significantly correlated with total antioxidant capacity (P<0.05), and the results could be used as an important indicator to predict the antioxidant activity of tea flowers.

     

/

返回文章
返回