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基于代谢组学辨析广西长寿饮食模式对机体的有益影响

韩坤宸 朱雯君 赵海燕 何潜祖 马金克 于晓涵 张钦任 李全阳

韩坤宸,朱雯君,赵海燕,等. 基于代谢组学辨析广西长寿饮食模式对机体的有益影响[J]. 食品工业科技,2022,43(15):9−18. doi:  10.13386/j.issn1002-0306.2022010237
引用本文: 韩坤宸,朱雯君,赵海燕,等. 基于代谢组学辨析广西长寿饮食模式对机体的有益影响[J]. 食品工业科技,2022,43(15):9−18. doi:  10.13386/j.issn1002-0306.2022010237
HAN Kunchen, ZHU Wenjun, ZHAO Haiyan, et al. Exploring the Beneficial Effects of Guangxi Longevity Dietary Patterns on Human Body Based on Metabolomics[J]. Science and Technology of Food Industry, 2022, 43(15): 9−18. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2022010237
Citation: HAN Kunchen, ZHU Wenjun, ZHAO Haiyan, et al. Exploring the Beneficial Effects of Guangxi Longevity Dietary Patterns on Human Body Based on Metabolomics[J]. Science and Technology of Food Industry, 2022, 43(15): 9−18. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2022010237

基于代谢组学辨析广西长寿饮食模式对机体的有益影响

doi: 10.13386/j.issn1002-0306.2022010237
基金项目: 国家自然科学基金面上项目(31871802);广西重点研发计划项目(桂科AB18221065)。
详细信息
    作者简介:

    韩坤宸(1996−),女,硕士研究生,研究方向:食品营养与健康长寿,E-mail:Ishankunchen@163.com

    通讯作者:

    李全阳(1964−),男,博士,教授,研究方向:食品营养与健康长寿,E-mail:liquanyang@gxu.edu.cn

  • 中图分类号: TS201.4

Exploring the Beneficial Effects of Guangxi Longevity Dietary Patterns on Human Body Based on Metabolomics

  • 摘要: 为了深入探究饮食与机体健康状态的关系,本研究试图从代谢组学的角度,评估所构建的广西长寿饮食模式对人体的有益影响。筛选志愿者在经过2周习惯性饮食后,高依从性地遵循这种饮食模式2周,在习惯性饮食前,广西长寿饮食前(习惯性饮食后)和广西长寿饮食后3次分别采集志愿者的血液和粪便样品,进行代谢组学分析。归纳所得结果发现,广西长寿饮食干预后志愿者血清组氨酸、甘油、α-葡萄糖和β-葡萄糖相对丰度显著增加(P<0.05),胆碱和乳酸的相对丰度显著降低(P<0.05)。此外还发现,粪便中的乙酸盐和丁酸盐含量显著上升(P<0.05)。代谢物组富集分析和途径分析结果表明,这些代谢物的变化可能与糖酵解/糖异生途径、丙酮酸代谢和半乳糖代谢3条途径密切相关。通过对差异代谢物的分析,认为广西长寿饮食模式对改善机体炎症水平、调节机体健康状态、预防机体罹患心血管疾病具有一定的作用。上述结果也从代谢组学角度对饮食促进机体健康长寿的关系提供了一定机制方面的阐释。
  • 图  1  样品采集时间序列图示

    Figure  1.  Illustration of sample collection time series

    图  2  志愿者习惯性饮食阶段和广西长寿饮食阶段的代表性CPMG 1H NMR血清光谱

    Figure  2.  Typical 500 MHz 1H NMR spectra of serum extracts for the volunteer with habitual diet and Guangxi longevity dietary

    注:与δ0.5~4.6相比,δ5.0~.4区域的光谱垂直扩大了6倍。1.脂质;2.异亮氨酸;3.亮氨酸;4.缬氨酸;5.乙醇;6.乳酸;7.丙氨酸;8.赖氨酸;9.乙酸;10.N-乙酰糖蛋白;11.谷氨酰胺;12.蛋氨酸;13.丙酮;14.乙酰乙酸盐;15.柠檬酸;16.三甲胺;17.肌酐;18. N-亚硝基二甲胺;19.胆碱;20.磷酸胆碱;21.三甲胺-N-氧化物;22.脯氨酸;23.鲨肌醇;24.甘氨酸;25.甘油;26.丝氨酸;27.苏氨酸;28. β-葡萄糖;29.α-葡萄糖;30.不饱和脂肪酸;31.组氨酸;32.酪氨酸;33.丙酮酸。

    图  3  血清1H NMR数据的多元分析

    Figure  3.  Multivariate data analysis of serum 1H NMR data

    注:(A)习惯性饮食前与广西长寿饮食干预前PCA模型得分图;(B)习惯性饮食后与广西长寿饮食干预后PCA模型得分图;(C)习惯性饮食后与广西长寿饮食干预后的OPLS-DA模型得分图;(D)置换检验图(200次)。

    图  4  代谢标志物的聚类分析及ROC分析

    Figure  4.  Cluster analysis and ROC analysis of potential biomarkers

    注:(A)代谢标志物聚类分析图;(B)代谢标志物的ROC分析。

    图  5  代谢标志物路径富集分析和拓扑分析

    Figure  5.  Metabolic marker pathway enrichment analysis and topology analysis

    表  1  基线时志愿者临床和人口统计学特征

    Table  1.   Clinical and demographic characteristics of volunteers at baseline

    基线特征参数
    年龄(岁)62.93±9.36
    性别(男/女)7/7
    BMI(kg/m225.42±3.75
    下载: 导出CSV

    表  2  血清中主要代谢物的统计分析结果

    Table  2.   Statistical analysis results of the main metabolite in serum

    代谢物
    (出峰位置)
    VIP相对丰度P
    习惯性饮食广西长寿饮食
    α-葡萄糖(5.23 ppm)1.151.41±0.291.65±0.25↑0.005
    β-葡萄糖(3.90 ppm)1.652.53±0.503.00±0.45↑0.006
    β-葡萄糖(3.43 ppm)2.928.51±1.6810.04±1.65↑0.005
    α-葡萄糖/β-葡萄糖
    (3.68~3.79 ppm)
    2.147.76±1.168.74±1.44↑0.008
    组氨酸(3.25 ppm)1.372.32±0.572.73±0.57↑0.017
    胆碱(3.20 ppm)2.605.54±1.594.23±1.57↓0.015
    N-乙酰糖蛋白
    (2.03 ppm)
    1.073.75±1.064.19±1.13↑0.053
    脂质(1.28 ppm)2.9523.69±7.4420.76±5.30↓0.086
    乳酸(4.11 ppm)1.161.85±0.551.54±0.38↓0.004
    乳酸(1.33 ppm)2.336.76±2.125.54±1.22↓0.017
    甘油(3.65 ppm)1.782.01±0.792.67±0.90↑0.020
    注:↑表示与习惯性饮食相比,在广西长寿饮食干预后代谢物相对丰度显著升高(P<0.05);↓表示与习惯性饮食相比,在广西长寿饮食干预后代谢物相对丰度有所降低(P<0.05)。
    下载: 导出CSV

    表  3  粪便SCFAs的统计分析结果

    Table  3.   Statistical analysis results of the main SCFAs in feces

    SCFAs相对含量(mmol/L)P
    Day 0Day 15Day 29Day 15 vs. Day 0Day 15 vs. Day 29
    乙酸30.03±5.4930.40±6.6537.64±4.90↑0.8260.005
    丙酸12.18±4.3912.34±7.2513.58±3.880.7300.221
    异丁酸1.22±0.601.32±0.431.78±0.960.3470.445
    丁酸15.63±6.6215.43±5.1324.51±7.02↑0.6830.004
    异戊酸1.17±0.681.15±0.561.54±1.260.7010.552
    戊酸0.98±0.461.05±0.571.55±1.080.3820.101
    注:↑表示与Day15(广西长寿饮食干预前)相比,Day29(广西长寿饮食干预后)SCFAs相对含量显著上升(P<0.05)。
    下载: 导出CSV
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出版历程
  • 收稿日期:  2022-01-26
  • 网络出版日期:  2022-06-17
  • 刊出日期:  2022-08-03

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