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中国精品科技期刊2020
肖宇,张涛,陈爱君,等. 玉米麸皮阿拉伯木聚糖提取工艺优化及其凝胶特性研究[J]. 食品工业科技,2023,44(15):166−174. doi: 10.13386/j.issn1002-0306.2022020191.
引用本文: 肖宇,张涛,陈爱君,等. 玉米麸皮阿拉伯木聚糖提取工艺优化及其凝胶特性研究[J]. 食品工业科技,2023,44(15):166−174. doi: 10.13386/j.issn1002-0306.2022020191.
XIAO Yu, ZHANG Tao, CHEN Aijun, et al. Optimization of Extraction Method of Arabinoxylan from Maize Bran and Analysis of Its Gel Properties[J]. Science and Technology of Food Industry, 2023, 44(15): 166−174. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022020191.
Citation: XIAO Yu, ZHANG Tao, CHEN Aijun, et al. Optimization of Extraction Method of Arabinoxylan from Maize Bran and Analysis of Its Gel Properties[J]. Science and Technology of Food Industry, 2023, 44(15): 166−174. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022020191.

玉米麸皮阿拉伯木聚糖提取工艺优化及其凝胶特性研究

Optimization of Extraction Method of Arabinoxylan from Maize Bran and Analysis of Its Gel Properties

  • 摘要: 为进一步挖掘玉米麸皮的附加价值、提升阿拉伯木聚糖(AX)的加工及应用,本研究以此为原料,采用碱法制备对其提取工艺进行优化,并探究其凝胶特性。以玉米深加工副产物麸皮为原料,采用Ba(OH)2提取AX,以料液比、提取温度、提取时间构建三因素三水平的响应面优化实验,并对优化后的多糖进行单糖组成、分子量、阿魏酸含量等结构表征。在此基础上,利用漆酶氧化交联AX,探究AX凝胶的形成条件、凝胶结构及热稳定性等参数。结果表明,优化后AX提取条件为料液比1:44(g:mL)、提取温度69 ℃、提取时间6.3 h,得率为23.07%±1.15%。制备得到的AX主要由阿拉伯糖和木糖组成,Ara/Xyl为2.28±0.11,重均分子量为265±6.68 kDa,阿魏酸含量为9.6±0.32 mg/100 g。AX经漆酶氧化交联后在pH2.0条件下可形成稳定的凝胶结构,扫描电镜观察微观结构为致密的多孔凝胶结构且具有良好的热稳定性。本研究制备的阿拉伯木聚糖及其凝胶具有良好的理化特性,在改善食品的质构品质、功能因子的包埋和控制释放等方面具有一定的应用潜力。本文为玉米加工副产物的深加工及阿拉伯木聚糖在食品工业中的应用提供理论基础。

     

    Abstract: In order to further explore the added value of corn bran and improve the processing and application of AX, this study used the corn bran as raw material, optimized its extraction process by alkaline preparation, and explored its gel characteristics. AX was extracted from bran, a by-product of deep processing of corn, by using Ba(OH)2. A response surface optimization with three factors and three levels was constructed based on the ratio of material to liquid, extraction temperature and extraction time. The structure of the optimized polysaccharide was characterized by monosaccharide composition, molecular weight and ferulic acid content. On this basis, AX was oxidized and cross-linked by laccase to explore the formation conditions, gel structure and thermal stability of AX gel. The results showed that the optimized extraction conditions of AX were as follows: Solid-liquid ratio 1:44 (g:mL), extraction temperature 69 ℃, extraction time 6.3 h, and the yield of AX was 23.07%±1.15%. The obtained AX was mainly composed of arabinose and xylose. The Ara/Xyl ratio was 2.28±0.11, the weight average molecular weight was 265±6.68 kDa, and the ferulic acid content was 9.6±0.32 mg/100 g. AX could form a stable gel structure under the condition of pH2.0 after laccase oxidation and cross-linking. The microstructure of AX was a dense porous gel structure and had good thermal stability according to scanning electron microscope. Arabinoxylan and its gel prepared in this study had good physical and chemical properties, and had potential application in improving the texture quality of food, embedding and controlled release of functional factors. This paper provides a theoretical basis for the further processing of corn by-products and the application of arabinoxylan in food industry.

     

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