Study of Lacticaseibacillus rhamnosus MP108 on Alleviating Bacterial Diarrhea
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摘要: 本文对含鼠李糖乳杆菌MP108产品对肠毒性大肠杆菌引起腹泻的缓解作用进行了评估。将六周龄雄性BALB/c小鼠分为空白组、模型组、益生菌MP108产品组和高剂量益生菌MP108产品组，益生菌干预持续两周。实验结束后，检测小鼠的粪便含水量、血清腹泻相关蛋白及炎症因子、空肠组织病理变化及肠道菌群的变化。结果显示MP108产品组和高剂量MP108产品组均能缓解肠毒性大肠杆菌（Enterotoxic Escherichia coli，ETEC）引起的腹泻症状，减少空肠组织损伤，降低血清炎症的水平，降低了铁蛋白（Serum ferritin，ST）和Toll样受体4（Toll-like receptor 4，TLR4）的表达，同时增加了水通道蛋白3（Aquaporin 3，AQP3）的水平。肠道菌群分析表明，鼠李糖乳杆菌MP108产品有助于恢复肠道的微生态，同时增加产短链脂肪酸的细菌Akkermansia，Ruminococcaceae，Lachnospiraceae的相对丰度。因此，鼠李糖乳杆菌MP108产品可通过调节腹泻相关蛋白、炎症因子的表达以及肠道菌群的变化发挥缓解细菌性腹泻的作用。Abstract: The alleviation effect of Lacticaseibacillus rhamnosus MP108-containing products on diarrhea caused by Enterotoxic Escherichia coli was evaluated. Six-week-old male BALB/c mice were randomly divided into blank group, model group, MP108 probiotic product group, and high-dose MP108 probiotic product group, and probiotic interventions lasted for two weeks. After the experiment, the fecal water content, serum diarrhea-related proteins, inflammatory factors, pathological changes of jejunum tissue, and gut microbial alterations were evaluated. The results showed that both the MP108 product group and the high-dose MP108 product group relieved diarrhea symptoms caused by Enterotoxic Escherichia coli (ETEC) via reducing jejunum damage, decreasing the levels of serum inflammatory factors, suppressing the expressions of serum ferritin (ST) and Toll-like receptor 4 (TLR4), and increasing aquaporin 3 (AQP3) levels. Intestinal flora analysis showed that L. rhamnosus MP108 product restored the intestinal microecology and increased the proportions of short-chain fatty acid-producing bacteria including Akkermansia, Ruminococcaceae, Lachnospiraceae. Collectively, L. rhamnosus MP108 probiotic products relieve diarrhea by regulating the expression of diarrhea-related proteins and inflammatory factors and modulating gut microbiota.
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Key words:
- Lacticaseibacillus rhamnosus /
- Escherichia coli /
- diarrhea /
- inflammatory factors /
- gut microbiota
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图 1 鼠李糖乳杆菌MP108菌株的耐酸耐胆盐能力
Figure 1. Acid and bile salt tolerance of Lacticaseibacillus rhamnosus MP108
注：与对照组比较，采用***（P<0.001）表示差异显著。
图 3 鼠李糖乳杆菌MP108产品对血清中腹泻相关蛋白含量的影响
Figure 3. Effects of L. rhamnosus MP108 product on diarrhea related protein content in serum
注：a免疫球蛋白A（IgA）；b水通道蛋白3（AQP3）；c铁蛋白（ST）；d Toll样受体4（TLR4）。
图 4 鼠李糖乳杆菌MP108产品对血清中细胞因子含量的影响
Figure 4. Effect of L. rhamnosus MP108 product on serum cytokine content
注：a肿瘤坏死因子α（TNF-α）；b-白介素6（IL-6）；c-白介素10（IL-10）；d-干扰素γ（IFN-γ）。
图 5 鼠李糖乳杆菌MP108产品对小鼠肠道菌群的影响
Figure 5. Effects of L. rhamnosus MP108 product on gut microbiota in mice
注：a基于OTU数量衡量菌群的α多样性（Observed）；b基于物种及丰度衡量菌群的α多样性化（Chao1）；c基于均匀度衡量菌群的α多样性化（Shannon）；d基于物种间进化关系衡量菌群的α多样性化（Simpson）；e菌群β多样性；f肠道菌群门水平的变化；g各组差异菌属比较。
表 1 实验分组及处理
Table 1. Experimental groups and treatments
组别 处理 第一周 第二周 第三周 前3 d 后4 d 空白对照组 适应期 生理盐水 链霉素 生理盐水 模型组 生理盐水 链霉素 生理盐水+ETEC MP108产品组 益生菌溶液 链霉素 益生菌溶液+ETEC 高剂量MP108产品组 益生菌溶液 链霉素 益生菌溶液+ETEC 
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