A Review of Methods and Progress in Highly Efficient Screening of Antimicrobial Peptides from Natural Products
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摘要: 面对耐药病原体的挑战，天然抗微生物肽（Antimicrobial peptide，AMP）成为了开发新一代抗菌候选药物的重要来源之一，在食品、农业等领域同样有着广泛的应用。然而，从天然产物中快速筛选获得抗微生物肽仍然存在着低效、高耗等种种困难与挑战。本文首先介绍了抗微生物肽的作用机制（包括膜作用机制与非膜作用机制）；然后重点综述了AMP的高效筛选方法，包括整体细菌吸附结合法、细胞膜色谱法、磷脂膜色谱法、毛细管电泳法、比色法、薄层色谱法、荧光筛选法、高通量测序法和数据库挖掘法等；此外，展望了高效发掘抗微生物肽的发展方向。本论文为从纷繁复杂的天然产物体系中发现抗微生物肽提供了可参考的科学依据。Abstract: Natural antimicrobial peptides (AMPs) are promising candidates for developing a generation of new antimicrobials to meet the challenge of antibiotic-resistant pathogens, and they are extensively applied in medicine, food, agriculture and other fields. However, the rapid screening of AMPs from natural products still has many difficulties and challenges, such as low efficiency and high consumption. This paper firstly introduces the action mechanism of AMPs (including membrane action mechanism and non-membrane action mechanism); then, the highly efficient screening methods of AMP are systematically reviewed, including bacterial adsorption, cell membrane chromatography, phospholipid membrane chromatography, capillary electrophoresis, colorimetry, thin layer chromatography, fluorescence screening, high-throughput sequencing, and mining databases screening. In addition, the development direction of efficient exploration of AMP is also prospected. This paper provides a scientific basis for the discovery of AMPs from complex natural product systems.
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表 1 AMP的高效筛选方法的汇总与比较
Table 1. Summary and comparison of efficient screening methods for AMP
筛选方法 AMP来源 筛选的AMP 整体细菌吸附结合法 废耗牛奶；
大鲵的血液Arg-Val-Met-Phe-Lys-Trp-Ala和Lys-Val-Ile-Ser-Met-Ile[33]；
andricin B[34]细胞膜色谱法 麻风树粕蛋白籽粕；鳀鱼提取物 JCpep7[35]；Apep10[36] 磷脂膜色谱法 卵蛋白水解物 Opep12[37] 毛细管电泳法 12条已知序列的阳离子AMP化合物
大白蛉幼虫血淋巴提取物HAL系列和HYL系列AMP[38]；
二肽 β-丙氨酰酪氨酸（β-Ala-Tyr）[39]比色法 磷脂和聚合二乙炔脂质组成的囊泡；亚洲海洋蛤蜊 K7L-毒蜂肽和W19-毒蜂肽[40]；Perinerin[41] 薄层色谱法 自行合成的10条粗肽 CAMEL和脂肽PAL-KK-NH2、Pal-KGK-NH2[42] 荧光筛选法 群居性蜜蜂（Lasioglossum laticeps）的毒液；
β-半乳糖苷酶Lasioglossin LL-III[43]；
肽GKH175W和KNK5W[44]高通量测序法 两栖类涂鱼 血红蛋白β1和淀粉样蛋白[45] 数据库挖掘法 AMP数据库；
牛乳肽数据库；
Fish-T1K数据库DASamP1[46]、
乳源AMP[47]、
小免疫肽[48]
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