铜藻多酚的分离纯化及抗氧化活性研究

何袅袅; 陈雅鑫; 蔡树芸; 施丽君; 陈伟珠; 陈晖; 洪专; 张怡; 张怡评

doi:10.13386/j.issn1002-0306.2022050220

铜藻多酚的分离纯化及抗氧化活性研究

doi: 10.13386/j.issn1002-0306.2022050220

何袅袅^{1, 2,},
陈雅鑫^{1, 2},
蔡树芸²,
施丽君²,
陈伟珠²,
陈晖²,
洪专^{1, 2},
张怡¹,
张怡评^2, ,

1.
福建农林大学食品科学学院，福建福州 350001
2.
自然资源部第三海洋研究所，海洋生物资源开发利用工程技术创新中心，福建厦门 361005

基金项目: 福建省科技计划项目（2021N0029）；自然资源部第三海洋研究所基本科研业务费专项资金资助项目（2022006）；福建省海洋经济发展资金项目（FJHJF-L-2021-4）；厦门海洋研究开发院共建项目（K210103）。

详细信息

作者简介:
何袅袅（1998−），女，硕士研究生，研究方向：食品加工与安全，E-mail：13887434960@163.com

通讯作者:
张怡评（1981−），男，博士，研究员，研究方向：海洋中药资源研究与开发，E-mail：ypzhang@tio.org.cn

中图分类号: TS201.1
计量
- 文章访问数: 51
- HTML全文浏览量: 15
- PDF下载量: 5
- 被引次数: 0
出版历程
- 收稿日期: 2022-05-19
- 网络出版日期: 2022-12-15
- 刊出日期: 2023-01-17

Separation and Purification and Antioxidant Activity of Polyphenols from Sargassum horneri

HE Niaoniao^{1, 2
,},
CHEN Yaxin^{1, 2},
CAI Shuyun²,
SHI Lijun²,
CHEN Weizhu²,
CHEN Hui²,
HONG Zhuan^{1, 2},
ZHANG Yi¹,
ZHANG Yiping^{2
, ,}

1.
College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350001, China
2.
Technical Innovation Center for Utilization of Marine Biological Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, China

摘要

摘要: 为研究铜藻多酚的分离纯化工艺及抗氧化活性。在超声辅助提取铜藻多酚的基础上采用大孔吸附树脂柱层析法分离纯化铜藻多酚提取物，以V_C为对照采用体外实验分析其抗氧化活性。结果显示：大孔吸附树脂LX-158具有最佳的吸附和解析条件，静态吸附和解析平衡时间为5 h，动态吸附和解析的最佳条件为：粗提液和洗脱剂流速为3 mL/min，上样体积为10 mL，洗脱剂为40%乙醇溶液，洗脱剂体积为120 mL。此条件下铜藻多酚纯度从7.52%提高到40.31%。体外抗氧化活性结果显示：不同浓度的铜藻多酚对DPPH自由基、ABTS自由基、超氧阴离子自由基、羟自由基有明显的清除作用和Fe³⁺还原力，随着多酚浓度增大其抗氧化能力增强，IC₅₀值分别为6.60 μg/mL、75.70 μg/mL、2.22 mg/mL、5.62 mg/mL。实验证明该纯化工艺可行且稳定，可以作为铜藻多酚纯化的工艺条件。
- 铜藻 /
- 多酚 /
- 分离纯化 /
- 大孔吸附树脂 /
- 抗氧化活性
Abstract: In order to study the separation and purification process and antioxidant activity of Sargassum horneri polyph-enols, on the basis of ultrasonic assisted extraction of Sargassum horneri polyphenols, macroporous adsorption resin column chromatography was used to separate and purify Sargassum horneri polyphenol extract, and V_C was used as the control to analyze its antioxidant activity in vitro. The results showed that the macroporous adsorption resin LX-158 exhibited the best adsorption and analysis conditions. The equilibrium time for static adsorption and analysis was 5 h. The following circumstances were ideal for dynamic adsorption and analysis: The flow rate of crude extract and eluent was 3 mL/min, the loading volume was 10 mL, the eluent was a 40% ethanol solution, and the eluent volume was 120 mL. The purity of Sargassum horneri polyphenols increased from 7.52% to 40.31% under these conditions. Antioxidant activity studies revealed that varied doses of Sargassum horneri polyphenols had noticeable scavenging effects on DPPH radical, ABTS radical, superoxide anion radical, hydroxyl radical and Fe³⁺ reducing power. With the increase of polyphenol concen-tration, its antioxidant capacity improved, and the IC₅₀ was 6.60 μg/mL, 75.70 μg/mL, 2.22 mg/mL, 5.62 mg/mL, respec-tively. The purification process is feasible and stable, and it can be used as a process condition for the purification of polyphenols from Sargassum horneri.
- Sargassum horneri /
- polyphenols /
- separation and purification /
- macroporous adsorption resin /
- antioxidant activity

HTML全文

图 1 两种大孔吸附树脂的吸附动力曲线

Figure 1. Adsorption dynamic curves of the two resins

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图 2 两种大孔吸附树脂的解析动力曲线

Figure 2. Analytical kinetic curves of the two resins

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图 3 LX-158大孔吸附树脂的吸附等温线

Figure 3. Adsorption isotherms of LX-158 resin

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图 4 流速对铜藻多酚吸附量的影响

Figure 4. Effect of flow rate on the adsorption capacity of Sargassum horneri polyphenols

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图 5 铜藻多酚粗提液泄露曲线

Figure 5. Leakage curve of Sargassum horneri polyphenol crude extract

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图 6 洗脱剂浓度对铜藻多酚解析率的影响

Figure 6. Effect of eluent concentration on the desorption capacity of polyphenols from Sargassum horneri

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图 7 洗脱剂流速对铜藻多酚解析率的影响

Figure 7. Effect of eluent flow rate on desorption capacity of polyphenols from Sargassum horneri

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图 8 铜藻多酚洗脱曲线

Figure 8. Elution curve of polyphenols of Sargassum horneri

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图 9 铜藻多酚在m/z为375的离子流色谱图

Figure 9. EIC figure of m/z=375 of polyphenols of Sargassum horneri

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图 10 Triphloretol A的结构式

Figure 10. The structural formula of Triphloretol A

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图 11 纯化前后铜藻多酚对DPPH自由基的清除能力

Figure 11. DPPH radical scavenging ability of Sargassum horneri polyphenols before and after purification

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图 12 纯化前后铜藻多酚对ABTS自由基的清除能力

Figure 12. ABTS radical scavenging ability of Sargassum horneri polyphenols before and after purification

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图 13 纯化前后铜藻多酚对超氧阴离子自由基的清除能力

Figure 13. Scavenging ability of Sargassum horneri polyphenols to superoxide anion radical before and after purification

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图 14 纯化前后铜藻多酚对羟自由基的清除能力

Figure 14. Scavenging ability of Sargassum horneri polyphenols to hydroxyl radical before and after purification

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图 15 纯化前后铜藻多酚对Fe³⁺的还原力

Figure 15. Reducing power of Sargassum horneri polyphenols to Fe³⁺ before and after purification

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表 1 11种大孔吸附树脂吸附和解析效果

Table 1. Effect of adsorption and desorption of 11 macroporous adsorption resins

树脂种类	吸附率（%）	吸附量（mg/g）	解析率（%）	解析量（mg/g）
X-15	35.33	2.48	62.10	1.54
LX-8	59.57	4.18	22.92	0.96
DM130	32.99	2.32	49.32	1.14
HP20	39.87	2.80	48.83	1.37
HI-816	52.00	3.65	67.62	2.47
NKA	26.82	1.88	47.13	0.89
NKA-9	53.16	3.73	77.07	2.88
AB-8	27.40	1.92	53.02	1.02
D101	39.41	2.77	51.25	1.42
D1400	30.78	2.16	50.98	1.10
LX-158	58.87	4.13	81.19	3.36

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