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中国精品科技期刊2020

响应面法优化微波膨化鱼糜制品料坯配方的研究

王蕾 王金厢 李学鹏 励建荣 杨青 位正鹏 季广仁

王蕾,王金厢,李学鹏,等. 响应面法优化微波膨化鱼糜制品料坯配方的研究[J]. 食品工业科技,2023,44(3):192−202. doi:  10.13386/j.issn1002-0306.2022050267
引用本文: 王蕾,王金厢,李学鹏,等. 响应面法优化微波膨化鱼糜制品料坯配方的研究[J]. 食品工业科技,2023,44(3):192−202. doi:  10.13386/j.issn1002-0306.2022050267
WANG Lei, WANG Jinxiang, LI Xuepeng, et al. The Optimization of the Billet Formulation of Microwave Expanded Surimi Products by Response Surface Methodology[J]. Science and Technology of Food Industry, 2023, 44(3): 192−202. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2022050267
Citation: WANG Lei, WANG Jinxiang, LI Xuepeng, et al. The Optimization of the Billet Formulation of Microwave Expanded Surimi Products by Response Surface Methodology[J]. Science and Technology of Food Industry, 2023, 44(3): 192−202. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2022050267

响应面法优化微波膨化鱼糜制品料坯配方的研究

doi: 10.13386/j.issn1002-0306.2022050267
基金项目: 国家重点研发计划课题(2019YFD0901605);辽宁省海洋经济发展专项(辽财指环[2022]33号)。
详细信息
    作者简介:

    王蕾(1997−),女,硕士研究生,研究方向:食品加工与安全,E-mail:wlwl0827@163.com

    通讯作者:

    李学鹏(1982−),男,博士,教授,研究方向:水产品贮藏与加工,E-mail:xuepengli8234@163.com

  • 中图分类号: TS254.4

The Optimization of the Billet Formulation of Microwave Expanded Surimi Products by Response Surface Methodology

  • 摘要: 以冷冻鱼糜为主要原料,通过单因素实验,考察淀粉种类、淀粉添加量、食盐添加量、膨松剂添加量、蛋清粉添加量对微波膨化鱼糜制品料坯的凝胶强度、膨化度、质构特性、感官评分的影响;采用Plackett-Burman试验筛选确定影响料坯品质的关键因素,通过Box-Behnken试验设计和响应面方差分析优化了微波膨化鱼糜制品料坯的最佳配方。结果显示,最适合添加到微波膨化鱼糜制品料坯中的淀粉种类是糯米淀粉,食盐添加量、糯米粉添加量和复配膨松剂添加量是影响料坯品质的主要影响因素。微波膨化鱼糜制品料坯的最佳配方为:以鱼糜质量为基准,食盐添加量2.16%、糯米粉添加量6.26%、复配膨松剂添加量0.49%、蛋清粉添加量0.9%,在此条件下料坯的凝胶强度为16729.33±270.72 g·mm,感官评分为85.8±0.84,与预测值无显著性差异(P>0.05)。研究为新型鱼糜制品的开发提供了参考依据。
  • 图  1  淀粉种类对鱼糜凝胶强度、料坯膨化度、感官评分的影响

    Figure  1.  Effect of starch types on gel strength, expansion ratio and sensory score of surimi billets expanded by microwave

    注:A:鱼糜凝胶强度;B:料坯膨化度;C:感官评分;不同小写字母表示差异显著(P<0.05),图2~图5同。

    图  2  食盐添加量对鱼糜凝胶强度、料坯膨化度、感官评分的影响

    Figure  2.  Effect of salt addition on gel strength, expansion ratio and sensory score of surimi billets expanded by microwave

    图  3  淀粉添加量对鱼糜凝胶强度、料坯膨化度、感官评分的影响

    Figure  3.  Effect of starch addition on gel strength, expansion ratio and sensory score of surimi billets expanded by microwave

    图  4  蛋清粉添加量对鱼糜凝胶强度、料坯膨化度、感官评分的影响

    Figure  4.  Effect of egg white powder addition on gel strength, expansion ratio and sensory score of surimi billets expanded by microwave

    图  5  复配膨松剂添加量对鱼糜凝胶强度、料坯膨化度和感官评分的影响

    Figure  5.  Effect of compound leavening agent addition on gel strength, expansion ratio and sensory score of surimi billets expanded by microwave

    图  6  各因素交互作用对鱼糜凝胶强度的影响

    Figure  6.  Effects of interactions between different parameters on gel strength of surimi

    图  7  各因素交互作用对感官评分的影响

    Figure  7.  Effects of interactions between different parameters on sensory score

    表  1  Plackett-Burman设计因素水平编码表

    Table  1.   Factors and levels of Plackett-Burman design

    编码水平因素
    A食盐添加量(%) B淀粉添加量(%)C蛋清粉添加量(%)D复配膨松剂添加量(%)
    −1130.30.3
    1390.90.9
    下载: 导出CSV

    表  2  Box-Behnken设计因素水平编码

    Table  2.   Factors and levels of Box-Behnken design

    编码水平因素
    A食盐添加量(%)B淀粉添加量(%)C复配膨松剂添加量(%)
    −1130.3
    0260.6
    1390.9
    下载: 导出CSV

    表  3  感官评价标准

    Table  3.   Sensory evaluation criteria

    项目外观口感色泽风味
    18~25分膨化好,气泡均匀;
    外观平整,厚薄一致
    硬度适中,弹性好色泽均匀,偏白色无鱼腥味,咸度适中
    9~17分膨化较好,气泡较均匀;
    外观较平整,厚薄较一致
    硬度较大,弹性较差色泽不均,无黄色略有鱼腥味,偏咸或偏淡
    0~8分未膨化,气泡不均匀;
    外观不平整,厚薄不均匀
    硬度过大,弹性差出现金黄色鱼腥味重,过咸
    下载: 导出CSV

    表  4  淀粉种类对料坯质构特性的影响

    Table  4.   Effects of starch types on texture of surimi billets expanded by microwave

    淀粉种类硬度(g)弹性(mm)黏聚性(g)咀嚼度(mJ)
    糯米淀粉3192.36±756.68d0.93±0.01bc0.72±0.02a2142.33±479.16b
    红薯淀粉4221.34±298.08ab0.97±0.01a0.76±0.07a3095.64±289.00a
    玉米淀粉4087.85±182.60bc0.96±0.03a0.76±0.06a2993.97±318.09a
    小麦淀粉4842.46±912.21ab0.93±0.01bc0.72±0.01a3228.82±598.55a
    马铃薯淀粉3270.67±172.05cd0.95±0.01ab0.76±0.05a2360.33±251.83b
    木薯淀粉4975.17±16.53a0.91±0.03c0.69±0.01a3149.01±169.43a
    注:同列不同字母表示差异显著(P<0.05),表5~表9表12同。
    下载: 导出CSV

    表  5  食盐添加量对料坯质构特性的影响

    Table  5.   Effect of salt addition on the texture of surimi billets expanded by microwave

    食盐添加量(%)硬度(g)弹性(mm)黏聚性(g)咀嚼度(mJ)
    017576.56±966.28a0.88±0.04ab0.71±0.03b10999.21±1119.99a
    19596.01±736.63d0.93±0.02a0.75±0.02ab6650.91±454.75b
    25110.29±701.63e0.94±0.03a0.82±0.09a3967.68±935.66c
    33876.37±610.27e0.93±0.01a0.74±0.02b2464.25±261.82c
    411099.55±1312.38c0.89±0.03ab0.74±0.06b7369.30±1479.80b
    512553.88±1184.24b0.84±0.05b0.76±0.08ab8107.51±1447.28b
    下载: 导出CSV

    表  6  淀粉添加量对料坯质构特性的影响

    Table  6.   Effect of starch addition on the texture of surimi billets expanded by microwave

    淀粉添加量(%)硬度(g)弹性(mm)黏聚性(g)咀嚼度(mJ)
    010491.66±1267.70b0.93±0.05a0.72±0.03b7098.71±1197.75a
    35163.93±176.24cd0.94±0.03a0.80±0.02a3913.62±189.84bc
    64347.71±79.28d0.91±0.01ab0.70±0.01b2762.02±119.03c
    95267.33±261.18cd0.91±0.01ab0.70±0.01b3336.06±217.38c
    126696.60±417.80c0.91±0.01ab0.82±0.02a5001.02±267.85b
    1512471.52±2268.20a0.86±0.04b0.70±0.01b7544.05±1060.92a
    下载: 导出CSV

    表  7  蛋清粉添加量对料坯质构特性的影响

    Table  7.   Effect of egg white powder addition on the texture of surimi billet expanded by microwave

    蛋清粉添加量(%)硬度(g)弹性(mm)黏聚性(g)咀嚼度(mJ)
    011310.59±940.25a0.92±0.02a0.74±0.07a7880.97±1538.11a
    0.312568.70±1371.41a0.90±0.05a0.74±0.02a8372.19±1504.75a
    0.68808.46±768.74b0.89±0.01a0.75±0.07a5938.57±1168.66b
    0.95256.43±308.26d0.94±0.02a0.77±0.06a3797.35±254.33c
    1.26222.98±156.15c0.92±0.01a0.80±0.07a4590.67±516.65bc
    1.57683.34±403.25b0.92±0.05a0.73±0.06a5209.51±556.00bc
    下载: 导出CSV

    表  8  复配膨松剂添加量对料坯质构特性的影响

    Table  8.   Effect of compound leavening agent addition on the texture of surimi billets expanded by microwave

    复配膨松剂添加量(%)硬度(g)弹性(mm)黏聚性(g)咀嚼度(mJ)
    09328.82±252.11bc0.93±0.01a0.77±0.05a6688.01±538.48ab
    0.39706.36±662.84b0.92±0.03a0.78±0.08a7025.43±1446.07a
    0.611997.80±694.65a0.88±0.05a0.76±0.06a8009.66±707.26a
    0.99887.59±57.34b0.92±0.03a0.81±0.09a7330.69±1035.67a
    1.28660.46±262.37c0.93±0.02a0.87±0.07a7067.57±563.77a
    1.57557.92±374.94d0.90±0.03a0.76±0.10a5206.97±1015.21b
    下载: 导出CSV

    表  9  Plackett-Burman试验设计与结果

    Table  9.   Plackett-Burman experimental design and results

    试验号A食盐添加量B淀粉添加量C蛋清粉添加量D复配膨松剂添加量鱼糜凝胶强度(g∙mm)感官评分(分)
    11−11−17650.42±118.89b77.2±0.45bc
    211−117664.12±530.83b78.2±1.30ab
    3−111−15138.22±42.62e69.0±1.00e
    41−1116383.64±207.97d76.4±0.55c
    511−117285.01±142.73bc78.4±1.52ab
    6111−18966.14±234.53a79.6±0.89a
    7−11114844.99±598.50e69.8±1.64e
    8−1−1113646.31±142.52f64.6±1.14f
    9−1−1−112727.51±186.77g65.8±0.84f
    101−1−1−16771.59±928.30cd76.4±0.55c
    11−11−1−15107.64±144.91e71.6±0.89d
    12−1−1−1−13695.51±405.57f69.6±1.14e
    下载: 导出CSV

    表  10  鱼糜凝胶强度回归模型方差分析

    Table  10.   ANOVA for the regression model of gel strength of surimi

    来源自由度平方和均方FP显著性
    模型44024835210062088142.230.00000088**
    A13188522631885226450.690.00000013**
    B15509637550963777.880.00004847**
    C195110295110213.440.0080006**
    D11902386190238626.890.00127309**
    残差749523270747
    失拟项6423369705620.980.64815887不显著
    纯误差17186271862
    总和265.984
    R20.9878
    R2Adj0.9809
    注:*:P<0.05,表示显著;**:P<0.01,表示极显著,表11表13表14同。
    下载: 导出CSV

    表  11  感官评分回归模型方差分析

    Table  11.   ANOVA for the regression model of sensory score

    来源自由度平方和均方FP显著性
    模型4299.3274.8357.460.00002**
    A1266.963266.963204.980.00000193**
    B120.80320.80315.970.00521**
    C11.471.471.130.32332
    D110.08310.0837.740.0272*
    残差79.1171.302
    失拟项69.0971.51675.810.08769不显著
    纯误差10.020.02
    总和1.14122
    R20.9704
    RAdj20.9536
    下载: 导出CSV

    表  12  响应面试验设计与结果

    Table  12.   Experiment design and results of response surface methodology

    试验号A食盐添加量B淀粉添加量C膨松剂添加量Y1鱼糜凝胶强度(g∙mm)Y2感官评分(分)
    100016355.90±406.39ab 83.4±2.07a
    21015609.31±221.65g77.2±0.84de
    30−1−111423.3±2091.57e79.4±0.55c
    40119384.88±373.41f77.4±0.89de
    500016481.04±607.57ab84.2±1.09a
    61−1010964.3±840.89e78±1.00cd
    700017246.2±1221.68a83±1.58ab
    801−114286±1571.60cd79.6±1.82c
    900016628.7±606.73ab82.6±1.67ab
    10−11015265.1±1139.89bc69.4±0.89g
    11−1−108420.91±494.30f70.6±1.14g
    120−119316.3±372.40f75.8±0.84e
    13−10−113520.7±816.13d72.6±1.34f
    1400016751.58±409.72ab82.8±1.48ab
    1510−111350.5±237.15e82.6±0.55ab
    16−10113137.6±699.21d73.2±1.09f
    171106416.82±140.01g81.4±1.14b
    下载: 导出CSV

    表  13  Y1回归模型方差分析

    Table  13.   ANOVA for the regression model of Y1

    来源自由度平方和均方FP显著性
    模型92.24E+082.49E+0784.73<0.0001**
    A13.20E+073.20E+07108.8<0.0001**
    B13.42E+063.42E+0611.610.0113*
    C12.16E+072.16E+0773.26<0.0001**
    AB13.24E+073.24E+07110.26<0.0001**
    AC17.18E+067.18E+0624.390.0017**
    BC11.95E+061.95E+066.630.0367*
    A214.62E+074.62E+07156.97<0.0001**
    B214.08E+074.08E+07138.75<0.0001**
    C212.58E+072.58E+0787.74<0.0001**
    残差72.06E+062.94E+05
    失拟项31.59E+065.29E+054.480.0907不显著
    纯误差44.72E+051.18E+05
    总和162.27E+08
    R20.9909
    RAdj20.9792
    下载: 导出CSV

    表  14  Y2回归模型方差分析

    Table  14.   ANOVA for the regression model of Y2

    来源自由度平方和均方FP显著性
    模型9353.5539.2881.6<0.0001**
    A1158.42158.42329.06<0.0001**
    B14.214.218.730.0212*
    C15.445.4411.310.012*
    AB15.295.2910.990.0129*
    AC13.613.617.50.029*
    BC10.040.040.0830.7815
    A2185.2685.26177.1<0.0001**
    B2162.4162.41129.64<0.0001**
    C2112.8912.8926.780.0013**
    残差73.370.48
    失拟项31.770.591.470.3483不显著
    纯误差41.60.4
    总和16356.92
    R20.9906
    RAdj20.9784
    下载: 导出CSV
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  • 收稿日期:  2022-05-23
  • 网络出版日期:  2022-12-15
  • 刊出日期:  2023-01-17

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