响应面优化藏羊皮胶原蛋白肽超声辅助提取工艺及其体内抗氧化活性分析

牛金鸽; 吴海玥; 马世科; 闫忠心; 王学江; 李婧; 胡蓉; 祁全青

doi:10.13386/j.issn1002-0306.2022060009

响应面优化藏羊皮胶原蛋白肽超声辅助提取工艺及其体内抗氧化活性分析

doi: 10.13386/j.issn1002-0306.2022060009

1.
青海大学农牧学院，青海西宁 810016
2.
青海大学畜牧兽医科学院，青海西宁 810016
3.
青海省乡村产业发展指导中心，青海西宁 810000

基金项目: 青海省昆仑英才高端创新创业人才项目（k992178）；“藏羊产品加工标准化体系建设与示范”项目；青海省科技厅项目（2021-SF-C22）；西宁市科技计划（2022-Y-11）。

详细信息

作者简介:
牛金鸽（1997−），女，硕士研究生，研究方向：食品科学，E-mail：1228643020@qq.com

通讯作者:
闫忠心（1987− ），男，博士，副研究员，研究方向：畜产品资源的加工利用，E-mail：421312454@qq.com

中图分类号: TS201
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- 文章访问数: 133
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- 被引次数: 0
出版历程
- 收稿日期: 2022-06-02
- 网络出版日期: 2023-04-20
- 刊出日期: 2023-06-01

Optimization of Ultrasonic Assisted Extraction of Tibetan Sheep Skin Collagen Peptide by Response Surface Methodology and Its Antioxidant Activity in Vivo

1.
College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China
2.
Qinghai Academy of Animal Science and Veterinary Science, Qinghai University, Xining 810016, China
3.
Qinghai Rural Industry Development Guidance Center, Xining 810000, China

摘要

摘要: 为明确超声辅助提取对藏羊皮胶原蛋白肽含量及功能活性的影响。试验采用响应面法研究了料液比、超声功率和超声时间3个因素，确定了胶原蛋白肽最佳超声辅助提取工艺；并对藏羊皮胶原蛋白肽进行了抗氧化能力分析，模拟消化过程中的还原力和·OH清除能力研究。结果表明：超声辅助处理能明显提高胶原蛋白肽含量，最佳超声提取工艺为料液比1:18、超声功率220 W，超声时间27 min，藏羊皮胶原蛋白肽含量为30.21%±1.67%。超声波辅助提取后胶原肽的还原力和·OH清除率为0.49和83.2%，与超声前的胶原肽相比分别提高了4%和38.3%，经胃肠消化后还原力和·OH均有一定程度的下降，但仍然具有较好的抗氧化活性。超声辅助提取藏羊皮胶原蛋白肽具有提高胶原蛋白肽含量和增强体内抗氧化活性的优势，为功能性产品的开发提供了数据参考。
- 胶原肽 /
- 超声辅助提取 /
- 响应面法 /
- 抗氧化活性
Abstract: In order to determine the effect of ultrasonic extraction on the content and functional activity of collagen peptide from Tibetan sheep skin, solid-liquid ratio, ultrasonic power and ultrasonic time were studied by response surface method to determine the best ultrasonic extraction process of collagen peptide. The antioxidant capacity, reduction power and ·OH scavenging ability of collagen peptide from Tibetan sheep skin was analyzed. The results showed that ultrasound-assisted treatment had significantly affected the content of collagen peptide and antioxidant activity. The optimal ultrasonic extraction conditions were solid-liquid ratio 1:18, ultrasonic power 220 W and ultrasonic time 27 min. The collagen peptide content of Tibetan sheep skin was 30.21%±1.67%. The reduction power and ·OH scavenging rate of collagen peptide after ultrasonic assisted extraction were 0.49 and 83.2%, which were increased by 4% and 38.3% compared with those before ultrasonic extraction, respectively. After gastrointestinal digestion, the reduction power and ·OH decreased to a certain extent, but still had good antioxidant activity. Ultrasound-assisted extraction of collagen peptide from Tibetan sheep skin has the advantages of increasing the content of collagen peptide and enhancing antioxidant activity in vivo, which provides data reference for the development of functional products.
- collagen peptide /
- ultrasonic-assisted extraction /
- response surface method /
- antioxidant activity

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图 1 料液比对藏羊皮胶原蛋白肽含量的影响

Figure 1. Influence of solid-liquid ratio on the content of Tibetan sheep skin collagen peptide

注：不同字母表示样品之间存在显著性差异（P<0.05）；图2、图3同。

下载: 全尺寸图片幻灯片

图 2 超声功率对藏羊皮胶原蛋白肽含量的影响

Figure 2. Effect of ultrasonic power on collagen peptide content of Tibetan sheep skin

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图 3 超声时间对藏羊皮胶原蛋白肽含量的影响

Figure 3. Effect of ultrasonic time on collagen peptide content of Tibetan sheep skin

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图 4 料液比、超声功率及超声时间对胶原蛋白肽含量影响的响应面

Figure 4. Response surface of the influence of solid-liquid ratio, ultrasonic power and ultrasonic time on collagen peptide content

下载: 全尺寸图片幻灯片

图 5 藏羊皮胶原蛋白肽的还原力

Figure 5. Reducing power of Tibetan sheep skin collagen peptide

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图 6 藏羊皮胶原蛋白肽的羟自由基清除能力

Figure 6. ·OH scavenging capacity of Tibetan sheep skin collagen peptide

下载: 全尺寸图片幻灯片

表 1 响应面试验因素水平设计

Table 1. Factors and levels of response surface test

水平	A	B	C
水平	料液比（g/mL）	超声功率（W）	超声时间（min）
−1	1:15	100	10
0	1:20	200	20
1	1:25	300	30

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表 2 藏羊皮基本成分

Table 2. Basic ingredients of Tibetan sheep skin

指标	水分	灰分	蛋白质	脂肪
含量（%）	65.89±0.57	0.27±0.02	21.2±0.14	15.0±0.07

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表 3 响应面分析方案及试验结果

Table 3. Response surface analysis scheme and test results

试验号	A 料液比	B 超声功率	C 超声时间	肽含量（%）
1	−1	0	1	24.89
2	0	1	1	28.39
3	0	0	0	31.98
4	−1	0	−1	25.31
5	0	−1	1	27.45
6	0	0	0	30.14
7	0	−1	−1	26.01
8	0	0	0	32.87
9	1	0	1	27.96
10	1	0	−1	26.39
11	−1	1	0	26.31
12	0	0	0	32.28
13	−1	−1	0	25.02
14	0	1	−1	30.9
15	1	1	0	26.98
16	0	0	0	30.71
17	1	−1	0	25.52

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表 4 胶原蛋白肽含量的响应面二次模型方差分析

Table 4. Response surface quadratic model variance analysis of collagen peptide content

来源	平方和	自由度	均方	F值	P值	显著性
模型	112.43	9	12.49	10.86	0.0024	**
A	3.54	1	3.54	3.07	0.1230
B	9.20	1	9.20	8.00	0.0255	*
C	8.000	1	8.00	6.952	0.9797
AB	7.225	1	7.225	6.279	0.9391
AC	0.99	1	0.99	0.86	0.3845
BC	3.90	1	3.90	3.39	0.1082
A²	62.22	1	62.22	54.07	0.0002	**
B²	13.56	1	13.56	11.78	0.0110	*
C²	10.97	1	10.97	9.53	0.0176
残差	8.06	7	1.15
失拟检验	2.91	3	0.97	0.75	0.5745
纯误差	5.14	4	1.29
总误差	120.48	16
	R²=0.9718			R²_adj=0.9356
注：*：差异极显著，P<0.01；：差异显著，P<0.05。

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