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中国精品科技期刊2020
顾璐萍,张钰,李俊华,等. 挤压膨化协同酶解工艺制备蛋壳膜多肽及其抗氧化活性和组成分析[J]. 食品工业科技,2022,43(23):252−258. doi: 10.13386/j.issn1002-0306.2022060043.
引用本文: 顾璐萍,张钰,李俊华,等. 挤压膨化协同酶解工艺制备蛋壳膜多肽及其抗氧化活性和组成分析[J]. 食品工业科技,2022,43(23):252−258. doi: 10.13386/j.issn1002-0306.2022060043.
GU Luping, ZHANG Yu, LI Junhua, et al. Analysis on Antioxidant Activity and Composition of Eggshell Membrane Peptides Prepared by Extrution and Enzymatic Hydrolysis[J]. Science and Technology of Food Industry, 2022, 43(23): 252−258. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022060043.
Citation: GU Luping, ZHANG Yu, LI Junhua, et al. Analysis on Antioxidant Activity and Composition of Eggshell Membrane Peptides Prepared by Extrution and Enzymatic Hydrolysis[J]. Science and Technology of Food Industry, 2022, 43(23): 252−258. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022060043.

挤压膨化协同酶解工艺制备蛋壳膜多肽及其抗氧化活性和组成分析

Analysis on Antioxidant Activity and Composition of Eggshell Membrane Peptides Prepared by Extrution and Enzymatic Hydrolysis

  • 摘要: 本文以蛋壳膜为研究对象,采用挤压膨化协同酶解工艺制备壳膜多肽。以总氮回收率、D-葡萄糖醛酸提取量及抗氧化性为指标,确定最佳工艺条件为:挤压膨化温度140 ℃,螺杆转速100 r/min,水分含量20%,酶解时间6 h,加酶量12000 U/g,温度55 ℃。在该条件下总氮回收率达60.8%,D-葡萄糖醛酸提取量达12.4 mg/g,壳膜多肽的ABTS自由基清除率为29.46%(0.1 mg/mL),OH自由基清除率为26.58%(5 mg/mL),Fe2+螯合能力为50.25%(0.4 mg/mL),细胞抗氧化活性达65%(10 μg/mL)。相对分子质量分布结果表明壳膜多肽中主要成分为低聚肽(252~2435 Da),占81.8%。氨基酸组成分析结果表明壳膜肽中Asp和Glu含量较高,与抗氧化活性有关的氨基酸含量为44.70 g/100 g蛋白。因此,蛋壳膜多肽有潜力作为天然抗氧化剂应用于食品、保健品或化妆品领域。

     

    Abstract: In this study, the eggshell membrane peptides were prepared using a two-step process involved extrution and enzymatic hydrolysis. The process parameters were determined by evaluation of the total nitrogen recovery, D-glucuronic acid yield and antioxidant activity. The optimum extrution process were 140 ℃ (temperature), 100 r/min (screw rotation speed) and 20% (moisture content). And the optimum conditions of enzymatic hydrolysis were 6 h (time), 12000 U/g (enzyme amount) and 55 ℃ (temperature). Under these conditions, the total nitrogen recovery and D-glucuronic acid yield were 60.8% and 12.4 mg/g, respectively. The ABTS, OH free radical scavenging ability and Fe2+ chelating ability of the shell membrane peptides were 29.46% (0.1 mg/mL), 26.58% (5 mg/mL) and 50.25% (0.4 mg/mL), respectively. And their cellular antioxidant activity reached 65% (10 μg/mL). Results on the molecular mass distribution indicated that the main components were oligopeptides (252~2435 Da), accounting for 81.8%. Analysis on the amino acid composition suggested a high level of Asp and Glu in the peptides, and the content of amino acids related with antioxidant activity was 44.70 g/100 g protein. And thus, the shell membrane peptides have great potentials as natural antioxidants in the field of food, healthy product and cosmetic.

     

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