低GI杂粮可可冲调粉辅助降血糖作用

那治国; 余爽; 贺书珍; 初众

doi:10.13386/j.issn1002-0306.2022070134

低GI杂粮可可冲调粉辅助降血糖作用

doi: 10.13386/j.issn1002-0306.2022070134

那治国^{1, 2,},
余爽²,
贺书珍³,
初众^3, ,

1.
哈尔滨商业大学食品工程学院，黑龙江哈尔滨 150028
2.
黑龙江东方学院食品工程学院，黑龙江哈尔滨 150066
3.
中国热带农业科学院香料饮料研究所，海南万宁 571533

基金项目: 农业农村部香辛饮料作物遗传资源利用重点实验室、海南省热带香料饮料作物工程技术研究中心开放课题资助（2019xys002）；黑龙江省重点研发计划指导类项目（GZ20210166）；黑龙江东方学院科研项目（HDFKY200101）。

详细信息

作者简介:
那治国（1980−），男，博士，教授，研究方向：粮油与植物蛋白加工，E-mail：nazhiguo1980@126.com

通讯作者:
初众（1981−），男，硕士，研究员，研究方向：食品加工与贮藏，E-mail：cz809@163.com

中图分类号: R587.1
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- 被引次数: 0
出版历程
- 收稿日期: 2022-07-14
- 网络出版日期: 2022-11-17
- 刊出日期: 2023-01-01

Auxiliary Hypoglycemic Effect of Low-GI Multigrain Cocoa Powder

NA Zhiguo^{1, 2
,},
YU Shuang²,
HE Shuzhen³,
CHU Zhong^{3
, ,}

1.
College of Food Engineering, Harbin University of Commerce, Harbin 150028, China
2.
College of Food Engineering, East University of Heilongjiang, Harbin 150066, China
3.
Chinese Academy of Tropical Agricultural Sciences, Spice and Beverage Research Institute, Wanning 571533, China

摘要

摘要: 目的：研究低GI杂粮可可冲调粉（Multigrain Cocoa Powder，C-MGP）对糖尿病小鼠血糖的影响。方法：52只雄性SPF级小鼠随机分为6组，包括正常对照组、模型组、二甲双胍组、冲调粉低、中、高剂量组（5%、10%、30% C-MGP）。正常对照组小鼠喂养基础饲料，模型组和二甲双胍组小鼠喂养高糖高脂饲料，冲调粉低剂量组（5% C-MGP）、中剂量组（10% C-MGP）、高剂量组（30% C-MGP）小鼠分别喂养含5%（质量分数）、10%（质量分数）、30%（质量分数）C-MGP的高糖高脂饲料。高脂饲料喂养四周后，小鼠腹腔注射30 mg/kg链脲佐菌素（STZ）建立Ⅱ型糖尿病小鼠模型。造模成功后，继续喂养4周，并测定小鼠基础指标和糖脂代谢等相关指标的变化。结果：经过高脂饲料联合STZ诱导，与模型组相比，10% C-MGP和30% C-MGP摄食量、饮水量均极显著降低（P<0.01），体重极显著升高（P<0.01）。与模型组相比，30% C-MGP显著降低了STZ诱导糖尿病小鼠的肝脏、肾脏指数，降低了空腹血糖水平（FBG）、糖化血红蛋白（GHb）、空腹胰岛素水平（FINS）及胰岛素抵抗指数（HOMA-IR），提高了葡萄糖耐量（GT），改善了血清中甘油三酯（TG）、总胆固醇（TC）、低密度脂蛋白胆固醇（LDL-C）、高密度脂蛋白胆固醇（HDL-C）和游离脂肪酸（FFA）水平，极显著促进了肝糖原（HG）合成（P<0.01），改善了肝脏脂肪堆积，极显著提高了血清胰高血糖素样肽（GLP-1）含量（P<0.01），极显著降低了血清脂多糖（LPS）水平（P<0.01）。结论：30% C-MGP具有调节STZ诱导糖尿病小鼠的糖脂代谢，减轻糖尿病症状的作用，具有进一步开发利用的价值。
- 杂粮 /
- 可可 /
- 降血糖 /
- Ⅱ型糖尿病 /
- 冲调粉
Abstract: Objective: To study the effect of low GI multigrain cocoa powder (C-MGP) on blood glucose in diabetic mice. Methods: Fifty-two male SPF mice were randomly divided into 6 groups, including normal control group, model group, metformin group, flushing powder low, medium and high-dose groups (5%, 10%, 30% C-MGP). Mice in normal control group were fed basal diet, mice in model group and metformin group were fed high-glucose and high-fat diets. Mice in low dose group (5% C-MGP), medium dose group (10% C-MGP) and high dose group (30% C-MGP) were fed high-glucose and high-fat diets containing 5% (mass fraction), 10% (mass fraction) and 30% (mass fraction) low GI mixed grain cocoa, respectively. After four weeks of high-fat diet, type Ⅱ diabetic mouse model was established by intraperitoneal injection of streptozotocin (STZ) (30 mg/kg). After successful modeling, feeding was continued for 4 weeks, basic indexes and glucose and lipid metabolism indexes were measured during the experiment in mice. Results: After high-fat diet combined with STZ induction, compared with the model group, 10% C-MGP and 30% C-MGP had significantly lower food intake and water intake (P<0.01), and significantly higher body weight (P<0.01). Compared with the model group, 30% C-MGP significantly decreased liver and kidney indexes, fasting blood glucose (FBG), glycosylated hemoglobin (GHb), fasting insulin (FINS) and insulin resistance index (HOMA-IR), and improved glucose tolerance (GT) in STZ-induced diabetic mice. Total glyceride (TG), total cholesterol (TC), low density lipid-cholesterol (LDL-C), high density lipid-cholesterol (HDL-C) and free fatty acids (FFA) levels were improved, hepatic glycogen (HG) synthesis was significantly promoted (P<0.01), and liver fat accumulation was improved. Significantly increased the content of serum glucagon like peptide-1 (GLP-1) (P<0.01), significantly decreased the level of serum lipopolysaccharide (LPS) (P<0.01). Conclusion: The 30% C-MGP had the effects of regulating glucose and lipid metabolism, and reduces diabetes symptoms on SZT induced diabetic mice, which is valuable for further development and utilization.
- coarse cereals /
- cocoa /
- hypoglycemic /
- type Ⅱ diabetes /
- mixed powder

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图 1 C-MGP对STZ诱导糖尿病小鼠体重、摄食和饮水的影响

Figure 1. Effect of C-MGP on body weight , food and water intakes in STZ-induced diabetic mice

注：正常对照组（n=12），模型、二甲双胍、C-MGP（n=8）；**P<0.01vs正常对照组，##P<0.01vs模型组，图2~图15同；A为小鼠体重周变化图，B为小鼠最终体重图，C为小鼠日摄食量变化图，D为小鼠日饮水量变化图。

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图 2 C-MGP对STZ诱导糖尿病小鼠脏器指数和脂肪质量的影响

Figure 2. Effects of C-MGP on visceral index and fat mass in STZ-induced diabetic mice

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图 3 C-MGP对STZ诱导糖尿病小鼠血糖水平的影响

Figure 3. Effect of C-MGP on blood glucose level in STZ-induced diabetic mice

注：A为空腹血糖值图；B为血糖下降比率图。

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图 4 C-MGP对STZ诱导糖尿病小鼠糖化血红蛋白的影响

Figure 4. Effect of C-MGP on GHb level in STZ-induced diabetic mice

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图 5 C-MGP对STZ诱导糖尿病小鼠葡萄糖耐受性的影响

Figure 5. Effect of C-MGP on glucose tolerance in STZ-induced diabetic mice

注：A为葡萄糖耐量图，B为曲线下面积图。

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图 6 C-MGP对STZ诱导糖尿病小鼠血清空腹胰岛素的影响

Figure 6. Effect of C-MGP on FINS in STZ-induced diabetic mice

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图 7 C-MGP对STZ诱导糖尿病小鼠胰岛素抵抗指数的影响

Figure 7. Effect of C-MGP on HOMA-IR in STZ-induced diabetic mice

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图 8 C-MGP对STZ诱导糖尿病小鼠胰高血糖素样肽-1的影响

Figure 8. Effect of C-MGP on GLP-1 in STZ-induced diabetic mice

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图 9 C-MGP对STZ诱导糖尿病小鼠血清甘油三酯的影响

Figure 9. Effect of C-MGP on serum TG in STZ-induced diabetic mice

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图 10 C-MGP对STZ诱导糖尿病小鼠血清总胆固醇的影响

Figure 10. Effect of C-MGP on serum TC in STZ-induced diabetic mice

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图 11 C-MGP对STZ诱导糖尿病小鼠血清低密度脂蛋白胆固醇的影响

Figure 11. Effect of C-MGP on serum LDL-c in STZ-induced diabetic mice

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图 12 C-MGP对STZ诱导糖尿病小鼠血清高密度脂蛋白胆固醇的影响

Figure 12. Effect of C-MGP on serum HDL-c in STZ-induced diabetic mice

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图 13 C-MGP对STZ诱导糖尿病小鼠血清游离脂肪酸的影响

Figure 13. Effect of C-MGP on serum FFA in STZ-induced diabetic mice

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图 14 C-MGP对STZ诱导糖尿病小鼠肝糖原含量的影响

Figure 14. Effect of C-MGP on HG contens in STZ-induced diabetic mice

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图 15 C-MGP对STZ诱导糖尿病小鼠血清脂多糖含量的影响

Figure 15. Effect of C-MGP on serum LPS contens in STZ-induced diabetic mice

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参考文献(33)

[1]	黄瑞, 刘敦华. 荞麦降糖酸奶的研制[J]. 粮油食品科技,2020,28(3):129−134. [HUANG R, LIU D H. Development of buckwheat hypoglycemic yogurt[J]. Science and Technology of Cereals, Oils and Foods,2020,28(3):129−134.
[2]	ANGELO L D. Estimated morbidity and mortality in adolescents and young adults diagnosed with type 2 diabetes mellitus[J]. Diabetic Medicine,2012,29(4):453−463. doi: 10.1111/j.1464-5491.2011.03542.x
[3]	SUGIHARA S. Pathophysiology, diagnosis and treatment of type 2 dia-betes mellitus in children and adolescent[J]. Nippon Rinsho Japanese Journal of Clinical Medicine,2012,70(5):31−37.
[4]	张继红, 马存根, 祝寿芬. 雀麦膳食纤维对2型糖尿病模型大鼠血糖、血脂影响的研究[J]. 山西大同大学学报(自然科学版),2008(3):64−67. [ZHANG J H, MA C G, ZHU S F. Effects of dietary fiber in oat bran on blood glucose and blood lipid in experimental type-2 diabetes mellitus rats[J]. Journal of Shanxi Datong University (Natural Science Edition),2008(3):64−67.
[5]	HASHEMI Z, FOUHSE J, IM H S, et al. Dietary pea fiber supplemen-tation improves glycemia and induces changes in the composition of gut microbiota, serum short chain fatty acid profile and expression of mucins in glucose intolerant rats[J]. Nutrients,2017,9(11):1236. doi: 10.3390/nu9111236
[6]	李兆钊, 吴卫国, 廖卢艳, 等. 杂粮中辅助调节血糖功效成分研究进展[J]. 中国粮油学报,2020,35(7):195−202. [LI Z Z, WU W G, LIAO L Y, et al. Research progress on functional components that help regulating of blood sugar in miscellaneous grains[J]. Journal of the Chinese Cereals and Oils Association,2020,35(7):195−202.
[7]	刘欣. 冲调粉的研制及其体外消化特性和抗氧化活性研究[D]. 天津: 天津农学院, 2019. LIU X. Development of brewing powder and its digestion characteristics and antioxidant activity in vitro[D]. Tianjin: Tianjin Agricultural University, 2019.
[8]	WU W, WANG L, QIU J, et al. The analysis of fagopyritols from tartary buckwheat and their anti-diabetic effects in KK-Ay type 2 diabetic mice and HepG2 cells[J]. Journal of Functional Foods,2018(4):137−146.
[9]	LIU M, ZHANG Y, ZHANG H, et al. The anti-diabetic activity of oat β-d-glucan in streptozotocin-nicotinamide induced diabetic mice[J]. International Journal of Biological Macromolecules,2016,91:1170−1176. doi: 10.1016/j.ijbiomac.2016.06.083
[10]	QIU J, L IU Y, YUE Y, et al. Dietary tartary buckwheat intake attenuates insulin resistance and improves lipidprofiles in patient s with type 2 diabetes: A randomized controlled trial[J]. Nutrition Research,2016,36(12):1392−1401. doi: 10.1016/j.nutres.2016.11.007
[11]	薛士科, 冯利. 红芸豆α-淀粉酶抑制剂和中草药组合饲料的应用价值[J]. 中国饲料,2020(24):52−54. [XUE S K, FENG L. The application value of red kidney bean α-amylase inhibitor and Chinese herbal medicine in the feed industry[J]. China Feed,2020(24):52−54.
[12]	章志, 熊艳. 白芸豆复方制剂对高血糖模型小鼠血糖的影响[J]. 实用中西医结合临床,2013,13(4):83−85. [ZHANG Z, XIONG Y. Effect of white kidney bean compound preparation on blood glucose in hyperglycemia model mice[J]. Practical Clinical Journal of Integrated Traditional Chinese and Western Medicine,2013,13(4):83−85.
[13]	黄修晴, 初众, 房一明, 等. 植物多酚降血糖机制的研究进展[J]. 食品工业科技: 2021, 42(18): 461-469. HUANG X Q, CHU Z, FANG Y M, et al. Research progress on hypoglycemic mechanism of plant polyphenols[J]. Science and Technology of Food Industry: 2021, 42(18): 461-469.
[14]	AONO Y, KAIDOT, KITA N, et al. Dose-dependent effects of cacao polyphenol intake on lipid metabolism in rats[J]. Plant Foods for Human Nutrition,2021(8):254−255.
[15]	MALKI A A. Oat attenuation of hyperglycemia-induced retinal oxidative stress and nf-kappa b activation in streptozotocin-induced diabetic rats[J]. Evid Based Complement Alternat Med,2013(1):983923−983930.
[16]	生庆海, 赵伟, 贾艳菊, 等. 复合营养粉对2型糖尿病大鼠的辅助降血糖试验[J]. 食品与机械,2020,36(12):147−151. [SHENG Q H, ZHAO W, JIA Y J, et al. Effects of compound nutrition powder on hypoglycemia in type 2 diabetic rats[J]. Food & Machinery,2020,36(12):147−151.
[17]	JI J, ZHANG C, LUO X, et al. Effect of stay-green wheat, a novel variety of wheat in China, on glucose and lipid metabolism in high-fat diet induced type 2 diabetic rats[J]. Multidisciplinary Digital Publishing Institute (MDPI),2015(7):5143−5155.
[18]	唐艺丹, 王鲜忠, 张姣姣. Ⅱ型糖尿病动物模型构建的研究进展[J]. 中国实验动物学报,2020,28(6):870−876. [TANG Y D, WANG X Z, ZHANG J J. Research progress in the construction of type II diabetes animal models[J]. Acta Laboratorium Animalis Scientia Sinica,2020,28(6):870−876.
[19]	祁瑜婷. 高粱淀粉制备及其功效评价研究[D]. 济南: 山东大学, 2018. QI Y T. Preparation and efficacy evaluation of sorghum starch[D]. Jinan: Shandong University, 2018.
[20]	郑晓丽, 阎利萍, 左吉卉, 等. 羊栖菜提取物的体外抗氧化活性及降低小鼠餐后血糖作用[J]. 现代食品科技,2020,36(6):33−39. [ZHENG X L, YAN L P, ZUO J H, et al. In vitro antioxidant activity and reduction of postprandial blood glucose by the extracts from Sargassum fusiforme[J]. Modern Food Science and Technology,2020,36(6):33−39.
[21]	何晓琴. 蒸汽爆破对苦荞麸皮膳食纤维理化特性及降血糖活性的影响[D]. 重庆: 西南大学, 2020. HE X Q. Effect of steam explosion on the physicochemical properties and hypoglycemic activity of Tartary buckwheat bran dietary fiber[D]. Southwest University, 2020.
[22]	廖作庄, 徐灵源, 王金妮, 等. 肉桂多酚对链脲佐菌素致糖尿病小鼠的保护作用[J]. 西安交通大学学报(医学版),2019,40(1):162−166. [LIAO Z Z, XU L Y, WANG J N. Protective effect of cinnamon polyphenols on streptozotocin induced diabetic mice[J]. Journal of Xi'an Jiaotong University (Medical Sciences),2019,40(1):162−166.
[23]	刘欣然, 康家伟, 王天星, 等. 菠萝蜜低聚肽对db/db小鼠炎症反应、血糖及血脂的影响[J]. 中国食物与营养,2020,26(4):61−65. [LIU X R, KANG J W, WANG T X, et al. Effects of jackfruit oligopeptides on inflammation, glucose and lipid in db/db diabetic mice[J]. Food and Nutrition in China,2020,26(4):61−65.
[24]	郭城, 冯元, 廖新茹, 等. 改性小麦麸膳食纤维对糖尿病小鼠的影响[J]. 安徽农业科学,2020,48(7):182−185. [GUO C, FENG Y, LIAO X R, et al. Effect of modified wheat bran dietary fiber on diabetic mice[J]. Journal of Anhui Agricultural Sciences,2020,48(7):182−185.
[25]	GAO R, WANG Y, WU Z, et al. Interaction of barley β-glucan and tea polyphenols on glucose metabolism in streptozotocin-induced diabetic rats[J]. Journal of Food Science,2012,77(6):H128−H134. doi: 10.1111/j.1750-3841.2012.02688.x
[26]	王德萍, 安馨, 鱼晓敏, 等. 鹰嘴豆醇提物降血糖作用研究[J]. 食品研究与开发,2019,40(13):21−25. [WANG D P, AN X, YU X M, et al. Hypoglycemic effect of chickpea ethanol extracts on diabetic mice[J]. Food Research and Development,2019,40(13):21−25.
[27]	高远, 杨鲁, 袁诗涵, 等. 虾青素酯对高脂高糖饮食致小鼠胰岛素抵抗的影响[J]. 食品工业科技,2019,40(23):290−295. [GAO Y, YANG L, YUAN S H, et al. Effects of astaxanthin ester on insulin resistance in mice induced by high fat and high sugar diet[J]. Science and Technology of Food Industry,2019,40(23):290−295.
[28]	QI W, WANG Y, SONG G, et al. Effects of four coarse cereals on blood glucose levels in rats with STZ-induced hyperglycemia[J]. Food & Agricultural Immunology,2019,30(1):487−496.
[29]	刘亚萍, 高文鸽, 梁隽杰, 等. 菊粉复配灵芝多糖对2型糖尿病大鼠的降血糖作用[J]. 食品工业科技,2019,40(20):310−315, 324. [LIU Y P, GAO W G, LIANG J J, et al. Hypoglycemic Effect of inulin combined with ganoderma lucidum polysaccharides on type 2 diabetes mellitus rats[J]. Science and Technology of Food Industry,2019,40(20):310−315, 324.
[30]	王语聪, 谢智鑫, 张学艳, 等. 阿拉伯半乳聚糖对2型糖尿病大鼠肠道碱性磷酸酶、细菌内毒素和肠道中细胞因子的影响[J]. 食品工业科技,2021,42(14):334−340. [WANG Y C, XIE Z X, ZHANG X Y, et al. Effect of arabinogalactan on intestinal alkaline phosphatase, bacterial endotoxin and serum cytokines in type 2 diabetic rats[J]. Science and Technology of Food Industry,2021,42(14):334−340.
[31]	PEREZ-RAMIREZ I F, BECERRIL-OCAMPO L J, REYNOSO-CAMACHO R, et al. Cookies elaborated with oat and common bean flours improvedserum markers in diabetic rats[J]. J Sci Food Agric,2018,98(3):998−1007. doi: 10.1002/jsfa.8548
[32]	尹雪倩, 张晓玄, 文婧, 等. 荞麦、燕麦、豌豆复配对糖尿病大鼠血糖的影响[J]. 北京大学学报(医学版),2021,53(3):447−452. [YIN X Q, ZHANG X X, WEN J, et al. Effects of the composite of buckwheat-oat-pea on blood glucose in diabetic rats[J]. Journal of Peking University (Health Sciences),2021,53(3):447−452.
[33]	傅樱花, 张富春, 彭永玉. 鹰嘴豆制品对糖尿病小鼠降血糖作用的研究[J]. 食品研究与开发,2016,37(4):26−28. [FU Y H, ZHANG F C, PENG Y Y. Hypoglycemic effects of chickpea products on diabetic mice[J]. Food Research and Development,2016,37(4):26−28.