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中国精品科技期刊2020

超高压处理对大豆拉丝蛋白特性的影响

张凯强 何晓叶 卫姣 袁芳

张凯强,何晓叶,卫姣,等. 超高压处理对大豆拉丝蛋白特性的影响[J]. 食品工业科技,2023,44(11):103−110. doi:  10.13386/j.issn1002-0306.2022070306
引用本文: 张凯强,何晓叶,卫姣,等. 超高压处理对大豆拉丝蛋白特性的影响[J]. 食品工业科技,2023,44(11):103−110. doi:  10.13386/j.issn1002-0306.2022070306
ZHANG Kaiqiang, HE Xiaoye, WEI Jiao, et al. Effects of High Pressure Processing Treatment on Properties of Drawing Soy Protein[J]. Science and Technology of Food Industry, 2023, 44(11): 103−110. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2022070306
Citation: ZHANG Kaiqiang, HE Xiaoye, WEI Jiao, et al. Effects of High Pressure Processing Treatment on Properties of Drawing Soy Protein[J]. Science and Technology of Food Industry, 2023, 44(11): 103−110. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2022070306

超高压处理对大豆拉丝蛋白特性的影响

doi: 10.13386/j.issn1002-0306.2022070306
详细信息
    作者简介:

    张凯强(1997−),男,硕士研究生,研究方向:功能性食品,E-mail:zhangkaiqiang2021@163.com

    通讯作者:

    袁芳(1967−),女,博士,副教授,研究方向:天然产物与功能食品,E-mail:yuanfang@cau.edu.cn

  • 中图分类号: TS201.1

Effects of High Pressure Processing Treatment on Properties of Drawing Soy Protein

  • 摘要: 本文研究了超高压处理对大豆拉丝蛋白特性的影响,以达到改善其再加工特性的目的。实验利用不同超高压处理条件(200~600 MPa,10~30 min)对大豆拉丝蛋白进行处理,采用傅里叶变换红外光谱、紫外吸收光谱和内源荧光光谱分析超高压对大豆拉丝蛋白结构的影响,并通过持水力、表面相对疏水性、游离巯基含量的变化研究超高压对大豆拉丝蛋白功能特性的影响。结果表明:随着压力和时间的增加,β-折叠、无规则卷曲相对含量上升,大豆拉丝蛋白的二级结构向着无序化方向进行;同时蛋白质三级结构伸展,内部疏水基团暴露量增多,但过大的压力和过长的时间则会使得疏水基团重新包埋。400 MPa、10 min时,大豆拉丝蛋白的持水力、游离巯基含量以及表面相对疏水性达到最大值,分别比对照组高32.87%、41.57%、15.66%。综上,适当条件的超高压处理有利于提高大豆拉丝蛋白再加工特性,这为超高压技术应用于大豆拉丝蛋白的生产提供参考。
  • 图  1  超高压处理前后DSP的傅里叶变换红外光谱

    Figure  1.  FTIR of DSP before and after high pressure processing treatment

    注:A:保压时间为10 min;B:保压时间为20 min;C:保压时间为30 min;图2~图4同。

    图  2  超高压处理前后DSP二级结构相对含量的变化

    Figure  2.  Changes of relative content of DSP secondary structure before and after high pressure processing treatment

    图  3  超高压处理前后DSP紫外吸收光谱的变化

    Figure  3.  Changes of UV absorption spectrum of DSP before and after high pressure processing treatment

    图  4  超高压处理前后DSP内源荧光光谱的变化

    Figure  4.  Changes of endogenous fluorescence spectrum of DSP before and after high pressure processing treatment

    图  5  超高压处理对DSP持水力的影响

    Figure  5.  Effects of high pressure processing treatment on water holding capacity of DSP

    注:小写字母不同表示不同压强和保压时间的处理组之间差异显著,P<0.05;图6~图7同。

    图  6  超高压处理前后DSP表面相对疏水性的变化

    Figure  6.  Changes of surface hydrophobicity of DSP before and after high pressure processing treatment

    图  7  超高压处理前后DSP游离巯基的变化

    Figure  7.  Changes of free SH content of DSP before and after high pressure processing treatment

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出版历程
  • 收稿日期:  2022-08-02
  • 网络出版日期:  2023-04-20
  • 刊出日期:  2023-06-01

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