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中国精品科技期刊2020

桑黄菌丝体多糖的分离纯化及抗氧化、抗肿瘤活性分析

李媛媛 李奉楠 杨小明 李岷

李媛媛,李奉楠,杨小明,等. 桑黄菌丝体多糖的分离纯化及抗氧化、抗肿瘤活性分析[J]. 食品工业科技,2023,44(11):127−135. doi:  10.13386/j.issn1002-0306.2022090180
引用本文: 李媛媛,李奉楠,杨小明,等. 桑黄菌丝体多糖的分离纯化及抗氧化、抗肿瘤活性分析[J]. 食品工业科技,2023,44(11):127−135. doi:  10.13386/j.issn1002-0306.2022090180
LI Yuanyuan, LI Fengnan, YANG Xiaoming, et al. Fractionation, Purification and Antioxidant, Antitumor Activity of Polysaccharides from Phellinus igniarius Mycelia[J]. Science and Technology of Food Industry, 2023, 44(11): 127−135. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2022090180
Citation: LI Yuanyuan, LI Fengnan, YANG Xiaoming, et al. Fractionation, Purification and Antioxidant, Antitumor Activity of Polysaccharides from Phellinus igniarius Mycelia[J]. Science and Technology of Food Industry, 2023, 44(11): 127−135. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2022090180

桑黄菌丝体多糖的分离纯化及抗氧化、抗肿瘤活性分析

doi: 10.13386/j.issn1002-0306.2022090180
基金项目: 国家自然基金(81372404);江苏省市场监督管理局基金(KJ21125110)。
详细信息
    作者简介:

    李媛媛(1985−),女,硕士,副主任药师,研究方向:中药活性成分提取及分析,E-mail:420518715@qq.com

    通讯作者:

    杨小明(1963−),女,博士,教授,研究方向:天然产物分离,E-mail:XM_Yang1963@126.com

    李岷(1967−),女,本科,主任技师,研究方向:微生物,E-mail:Lmnanjing@163.com

  • 中图分类号: TS255.1

Fractionation, Purification and Antioxidant, Antitumor Activity of Polysaccharides from Phellinus igniarius Mycelia

  • 摘要: 采用水提醇沉法制备桑黄菌丝体粗多糖,分别用终浓度为30%、45%和60%的硫酸铵溶液对桑黄菌丝体中粗多糖进行粗分离,并比较所得各多糖组分的抗氧化、抗肿瘤活性。对其中活性强的组分采用离子交换树脂柱色谱纯化,采用高效排阻色谱-折光示差检测器-多角度光散射仪对纯化多糖进行均一性分析和分子量测定。结果显示,45%硫酸铵分离多糖IPS45显示最强的抗肿瘤活性及较好的清除超氧阴离子活性,IPS45经DEAE-52纤维素离子交换柱纯化得到四个级分,经高效排阻色谱分析,其中蒸馏水洗脱级分(IPS45-W)为均一多糖,重均分子量为79.1 kDa;气相色谱分析其单糖组成为甘露糖:葡萄糖:半乳糖:未知单糖,摩尔比为1.57:8.38:1.09:1.00。IPS45-W可抑制HepG2细胞生长,当浓度为50 μg/mL时,对HepG2细胞的抑制率为56.74%,高于前期研究的乙醇沉淀分离所得桑黄菌丝体均一多糖同浓度下11%~35%的抑制率。该结果为不同组成的桑黄多糖的分离和应用提供依据。
  • 图  1  粗多糖及各上清液组分的抗氧化能力

    Figure  1.  Antioxidant capacity of crude polysaccharide and supernatant fractions

    注:A:超氧阴离子清除能力;B:羟基自由基清除能力;C:还原能力。

    图  2  各多糖组分对HepG2细胞的抑制作用

    Figure  2.  Inhibition of polysaccharides on HepG2 cells

    注:*表示同一浓度不同样品间差异显著(P<0.05)。

    图  3  IPS45的离子柱层析色谱图

    Figure  3.  Elution curve of IPS45 on DEAE-52 column chromatography

    图  4  IPS45-W的高效凝胶渗透色谱图

    Figure  4.  SEC-RI-MALLS chromatograms of IPS45-W

    注:RI:折光示差;LS:光散射。

    图  5  标准单糖(A)和IPS45-W(B)的气相色谱图

    Figure  5.  GC chromatography of standard monosaccharide (A) and IPS45-W (B)

    图  6  DEAE-52分离所得各多糖组分对HepG2细胞的抑制作用

    Figure  6.  Inhibition of polysaccharides fractionations from DEAE-52 on HepG2 cells

    注:*表示差异显著(P<0.05);**表示差异极显著(P<0.01)。

    表  1  粗多糖各组分的得率、多糖和蛋白质的含量

    Table  1.   Yields and contents of carbohydrate and protein of fractions from crude polysaccharides

    样品名称IPS30IPS45IPS60IPP30IPP45IPP60
    得率(%)16.727.866.5
    多糖含量(%)18.7329.0334.689.038.5010.25
    蛋白质含量(%)3.032.472.005.025.175.41
    注:“−”表示未检测。
    下载: 导出CSV

    表  2  IPS45经DEAE-52纯化后各级分的多糖与蛋白含量

    Table  2.   The content of polysaccharides and proteins of IPS45 fractions purified by DEAE-52

    组分得率(%)多糖含量(%)蛋白含量(%)
    IPS45-W30.078.080.33
    IPS45-17.047.890.65
    IPS45-24.030.650.17
    IPS45-31.627.940.57
    下载: 导出CSV
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  • 收稿日期:  2022-09-19
  • 网络出版日期:  2023-04-20
  • 刊出日期:  2023-06-01

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