基于凹凸棒稳定的姜精油纳米乳的性质表征和结肠靶向研究

农家慧; 蒋倩; 孔令艳; 薛梅; 雷红

doi:10.13386/j.issn1002-0306.2022100204

基于凹凸棒稳定的姜精油纳米乳的性质表征和结肠靶向研究

doi: 10.13386/j.issn1002-0306.2022100204

南京财经大学食品科学与工程学院，江苏省现代粮食流通与安全协同创新中心，江苏南京 210023

基金项目: 国家自然科学基金面上项目（Grant No. 31872899）；江苏高校优势学科建设工程资助项目（PAPD）

详细信息

作者简介:
农家慧（1999−），女，硕士研究生，研究方向：食品功能因子，E-mail：nongjiahui2021@163.com

通讯作者:
雷红（1973−），女，博士，教授，研究方向：食品功能因子，E-mail：leihong56@163.com

中图分类号: TS201.7
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- 文章访问数: 128
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出版历程
- 收稿日期: 2022-10-21
- 网络出版日期: 2023-03-30
- 刊出日期: 2023-06-01

Study on Characterization and Colonic Targeting of Ginger Essential Oil Nanoemulsion Stabilized by Palygorskite

College of Food Science and Engineering, Nanjing University of Finance and Economics, Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing 210023, China

摘要

摘要: 为提高姜精油（Ginger essential oil, GEO）的稳定性和利用度，本实验室以酸改性凹凸棒为稳定剂、亚麻籽胶作为乳化剂、壳聚糖盐酸盐做壁材制备了姜精油纳米乳。本研究对姜精油纳米乳进行性质表征（包括形貌与形态、粒径分布、流变性、红外光谱、包埋率）并评价其结肠靶向和缓释性能。研究结果表明姜精油纳米乳粒径呈正态分布，液滴平均粒径为329.6 nm，PDI为0.259，包埋率为91.26%±0.03%；通过荧光显微镜和扫描电镜观察，表明所制备姜精油纳米乳分散度良好，呈表面光滑的球形；红外光谱结果表明，姜精油被包封进纳米乳中且化学组成未被破坏，纳米乳中各组分的结合方式为物理结合；姜精油纳米乳在 4、25、40 ℃贮藏28 d稳定性良好；通过体外模拟消化试验及动力学分析结果表明，姜精油纳米乳具有结肠靶向和缓释的性能。本研究为食品功能因子结肠递送体系的建立、以及后期姜精油纳米乳作为食品功能因子改善肠道健康中的应用提供了理论依据。
- 姜精油 /
- 纳米乳 /
- 性质表征 /
- 贮藏稳定性 /
- 结肠靶向
Abstract: To improve the stability and utilization rate of ginger essential oil (GEO), GEO nanoemulsion stabilized by acid-modified attapulgite, emulsified by flaxseed gum and embedded by chitosan hydrochloride were prepared in this laboratory. The characteristics of GEO-loaded nanoemulsion were investigated, such as lmorphology, topography, particle size distribution, rheology, embedding rate, Fourier-transform infrared spectroscopy (FT-IR), as well as the release mechanisms. Results showed that the particle size of GEO nanoemulsion was normally distributed, the average droplet size was 329.6 nm, the polydispersity index (PDI) was 0.259 and the embedding rate was 91.26%±0.03%. GEO nanoemulsion had good dispersion and smooth spherical surface investigated by scanning electron microscope and fluorescence microscope. The results of FT-IR showed that GEO was encapsulated in the nanoemulsion and the binding mode was physical interaction, while its chemical composition did not changed. GEO nanoemulsion had good stability when stored at 4, 25 and 40 ℃ for 28 days. GEO nanoemulsion had colonic targeting and sustained release properties investigated by in vitro simulated digestion test and kinetic analysis. This study provided a theoretical basis for the establishment of the colonic delivery system of food functional factors, and the application of GEO nanoemulsion as food functional factors to improve intestinal health.
- ginger essential oil (GEO) /
- nanoemulsion /
- property characterization /
- storage stability /
- colon targeting

HTML全文

图 1 姜精油纳米乳的荧光显微图（1000×）

Figure 1. Fluorescence micrograph of ginger essential oil nanoemulsion (1000×)

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图 2 姜精油纳米乳的扫描电镜图

Figure 2. Scanning electron microscope image of ginger essential oil nanoemulsion

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图 3 姜精油纳米乳的粒径分布图

Figure 3. Particle size distribution diagram of ginger essential oil nanoemulsion

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图 4 姜精油纳米乳在不同剪切率下的粘度变化图

Figure 4. Viscosity change chart of ginger essential oil nanoemulsion at different shear rates

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图 5 姜精油纳米乳（GEO-NE）及其各组分凹凸棒（PGS）、亚麻籽胶（FG）、姜精油（GEO）、壳聚糖盐酸（CHC）的红外光谱图

Figure 5. Infrared spectra of ginger essential oil nanoemulsion (GEO-NE) and its components attapulgite (PGS), linseed gum (FG), ginger essential oil (GEO) and chitosan hydrochloride (CHC)

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图 6 姜精油纳米乳体外缓释累积释放曲线

Figure 6. Cumulative release curve of ginger essential oil nanoemulsion in vitro

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图 7 姜精油纳米乳模拟体外释放的荧光显微图

Figure 7. Fluorescence micrograph of simulated in vitro release of ginger essential oil nanoemulsion

注：比例尺：10 μm，倍数：1000倍；从左至右为：模拟胃液，模拟小肠液，模拟结肠液。

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表 1 不同储存条件下姜精油纳米乳平均粒径和Zeta电位的变化

Table 1. Changes of Z-average and Zeta potential of ginger essential oil nanoemulsion at different storage conditions

天数（d）	指标
	平均粒径（nm）			Zeta电位（mV）
	4 ℃	25 ℃	40 ℃	4 ℃	25 ℃	40 ℃
1	324.9±2.9^a	329.8±2.1^a	344.8±19.2^a	−39.0±0.8^bc	−39.3±0.5^c	−40.4±0.4^b
3	388.0±6.3^b	389.3±10.4^b	411.4±12.6^b	−38.4±0.4^cd	−39.5±1.2^c	−32.0±0.8^c
7	398.2±6.2^b	400.8±15.5^bc	424.1±3.9^b	−39.3±0.8^bc	−39.2±1.3^c	−39.4±1.1^b
14	425.2±10.5^c	439.0±2.6^c	469.8±29.0^c	−37.7±0.8^d	−42.6±1.4^b	−39.2±0.5^b
21	430.8±9.0^c	445.1±34.4^cd	500.1±8.9^cd	−39.7±0.4^b	−44.5±0.5^a	−42.7±0.7^a
28	453.6±18.7^d	461.2±37.9^d	520.8±32.7^d	−41.9±0.9^a	−44.4±1.4^a	−40.0±1.6^b
注：表格中数据表示为平均值±标准差。不同小写字母表示在相同温度下样品的平均粒径和Zeta电位随着天数的变化有显著差异（P<0.05）。

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表 2 姜精油纳米乳体外模拟结肠消化释放动力学方程拟合

Table 2. Kinetic equation fitting of ginger essential oil nanoemulsion in vitro simulating colon digestion and release

动力学模型	方程	K	n	R²
Zero-order	Q=Kt+C	2.2079	2.1548	0.9541
First-order	Q=C(1-exp(-Kt))	294.4363	0.0095	0.9193
Higuchi	Q=Kt^1/2+C	10.6912	−7.2047	0.7448
Rigter-Peppas	Q=Kt^C	4.4119	0.872	0.9648
注：Q 代表累积释放率，t 代表消化时间，R²代表动力学模型决定系数，K 为释放速率常数，n 为扩散指数。

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