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中国精品科技期刊2020
曹涓泉,李心悦,徐静,等. 圆苞车前子壳粉对鸡肉肌原纤维蛋白凝胶特性和蛋白结构的影响[J]. 食品工业科技,2023,44(17):100−107. doi: 10.13386/j.issn1002-0306.2022110120.
引用本文: 曹涓泉,李心悦,徐静,等. 圆苞车前子壳粉对鸡肉肌原纤维蛋白凝胶特性和蛋白结构的影响[J]. 食品工业科技,2023,44(17):100−107. doi: 10.13386/j.issn1002-0306.2022110120.
CAO Juanquan, LI Xinyue, XU Jing, et al. Effects of Psyllium Husk Powder on Gel Properties and Protein Structure of Chicken Myofibrillar Protein[J]. Science and Technology of Food Industry, 2023, 44(17): 100−107. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022110120.
Citation: CAO Juanquan, LI Xinyue, XU Jing, et al. Effects of Psyllium Husk Powder on Gel Properties and Protein Structure of Chicken Myofibrillar Protein[J]. Science and Technology of Food Industry, 2023, 44(17): 100−107. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022110120.

圆苞车前子壳粉对鸡肉肌原纤维蛋白凝胶特性和蛋白结构的影响

Effects of Psyllium Husk Powder on Gel Properties and Protein Structure of Chicken Myofibrillar Protein

  • 摘要: 本试验旨在研究不同质量浓度的圆苞车前子壳粉(psyllium husk powder,PHP)对肌原纤维蛋白(myofibrillar protein,MP)-PHP复合凝胶体系的凝胶特性以及蛋白结构的影响。将鸡肉MP浓度稀释至40 mg/mL,分别加入0%、0.5%、1%、1.5%、2%(w/v)的PHP,使混合体系在80 ℃下水浴30 min形成MP-PHP复合凝胶,通过测定复合凝胶的凝胶强度、保水性、动态流变学特性、水分分布特性等指标来研究PHP对鸡肉MP凝胶特性的影响;此外,通过MP活性巯基含量、表面疏水性及二级结构来探究蛋白结构的变化。结果表明:当PHP添加量在0%~1.5%范围内增加时,MP-PHP复合凝胶的保水性和凝胶强度分别逐渐增加到80.51%和73.79 g·mm;而继续增加圆苞车前子壳粉添加量至2%时,其保水性和凝胶强度仅小幅波动。此外,PHP的添加使复合凝胶的粘弹性明显提升,其中,1.5%的添加量效果最佳。圆二色谱结果显示,添加1.5% PHP能够促使MP的α-螺旋和无规则卷曲向β-折叠与β-转角转变,这有利于凝胶功能特性的改善。因此,添加适量脂肪替代物PHP可以改善MP的凝胶特性及蛋白结构性质,这对于鸡肉制品品质改善具有重要意义。

     

    Abstract: This experiment was conducted to study the effects of various amounts of psyllium husk powder (PHP) on the gel properties and protein structure of the myofibrillar protein-PHP (MP-PHP) composite gel system. The PHP at 0%, 0.5%, 1%, 1.5% and 2% concentrations was added to MP diluted to 40 mg/mL to form different MP-PHP systems subjected to a water bath at 80 ℃ for 30 min. The effects of PHP on chicken MP gel properties were investigated by measuring gel strength, water retention, dynamic rheological properties and water distribution characteristics of composite gel. On the other hand, the changes of protein structure were investigated by MP active sulfhydryl group, surface hydrophobicity, and secondary structure determination. Results showed that when the addition of PHP increased in the range of 0% to 1.5%, the water retention and gel strength of MP-PHP composite gel gradually increased to 80.51% and 73.79 g·mm, respectively. However, when the addition of PHP was increased to 2%, the water retention and gel strength fluctuated only slightly. Meanwhile, the addition of PHP significantly improved the viscoelasticity of the composite gel, among which 1.5% was the best. Furthermore, the results of circular dichroism showed that the transformation of α-helix and random curling to β-folding and β-turning of MP benefited from the addition of 1.5% PHP, which improved the functional properties of the gel. Therefore, adding appropriate amount of PHP is an effective way to improve the gel properties and protein structural properties of MP, which is of great significance for improving the quality of chicken products.

     

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