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中国精品科技期刊2020
管婷婷,寇宇,张一婷,等. 不同豆角品种生长期及发芽处理对其皂苷含量的影响[J]. 食品工业科技,2023,44(20):70−76. doi: 10.13386/j.issn1002-0306.2022120108.
引用本文: 管婷婷,寇宇,张一婷,等. 不同豆角品种生长期及发芽处理对其皂苷含量的影响[J]. 食品工业科技,2023,44(20):70−76. doi: 10.13386/j.issn1002-0306.2022120108.
GUAN Tingting, KOU Yu, ZHANG Yiting, et al. Effects of Growth Period and Germination Treatment on Saponin Content of Different Bean Varieties[J]. Science and Technology of Food Industry, 2023, 44(20): 70−76. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022120108.
Citation: GUAN Tingting, KOU Yu, ZHANG Yiting, et al. Effects of Growth Period and Germination Treatment on Saponin Content of Different Bean Varieties[J]. Science and Technology of Food Industry, 2023, 44(20): 70−76. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022120108.

不同豆角品种生长期及发芽处理对其皂苷含量的影响

Effects of Growth Period and Germination Treatment on Saponin Content of Different Bean Varieties

  • 摘要: 为明确东北油豆角皂苷的含量、存在部位及其影响因素,以提高油豆角系列产品的加工利用价值,本研究对开花后10~40 d内的金冠、绿冠、盛冠等7种东北油豆角采用超声辅助法分别提取豆角种子及荚皮中的皂苷,并利用0.4%~1.2%的氯化钠溶液进行盐胁迫发芽处理,分析其对金冠和满堂彩豆角种子皂苷含量的影响,最后采用超高效液相色谱-四极杆-飞行时间质谱技(UHPLC-Q-TOF-MS)对金冠豆角种子中的皂苷种类进行鉴定。结果表明,豆角皂苷主要存在部位为豆角种子,且皂苷含量随生长时间的增加呈现先上升后下降的趋势,皂苷含量出现高峰的时期因豆角品种而异,与其他6个品种豆角相比,满堂彩中皂苷含量最高,在35 d时,整个豆角皂苷含量为(23.62±0.14)mg/g。皂苷含量变化与豆粒萌芽时间、豆角品种密切相关,盐胁迫处理可以使满堂彩种子皂苷含量由(7.08±0.15)mg/g提高到(10.15±0.55)mg/g。UHPLC-Q-TOF-MS分析表明金冠豆角粗皂苷中存在柴胡皂苷F、赤豆皂苷II、黄芪皂苷IV等7种皂苷。本研究为东北油豆角皂苷的深入开发提供数据支持。

     

    Abstract: In order to improve the processing and utilization value of the Northeast snap beans products by clarifying the saponin content, sites and influencing factors, this study extracted saponins from the seeds and pods of seven kinds of Northeast snap beans, including Golden Crown, Green Crown, and Sheng Crown within 10~40 d after blooming using ultrasonic-assisted methods. 0.4%~1.2% sodium chloride solution was used to conduct germination treatment under salt stress on the saponin content of Golden Crown and Mantangcai seed. Finally, ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS) was used to identify the saponin species in the seeds of Golden Crown. The results showed that saponin existed mainly in the seeds, and the saponin content increased firstly but then decreased with the increase of growth time. The peak period of saponin content varied with the bean varieties. Compared with the other six varieties of snap beans, the saponin content in the full hall was the highest, with the highest saponin content of Mantangcai whole pods of 23.62±0.14 mg/g at 35 d. The change of saponin content was closely related to the sprouting time of bean seeds and bean varieties. Salt stress treatment could significantly increase the saponin content of Mantangcai seeds from (7.08±0.15) mg/g to (10.15±0.55) mg/g. The UHPLC-Q-TOF-MS analysis showed that there were seven saponins in the crude saponins of the Golden Crown bean, including saikosaponin F, adzuki saponin II and astragaloside IV. This study provides data support for the in-depth development of soybean saponins in Northeast China.

     

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