• EI
  • Scopus
  • 食品科学与工程领域高质量科技期刊分级目录第一方阵T1
  • DOAJ
  • EBSCO
  • 北大核心期刊
  • 中国核心学术期刊RCCSE
  • JST China
  • FSTA
  • 中国精品科技期刊
  • 中国农业核心期刊
  • CA
  • WJCI
  • 中国科技核心期刊CSTPCD
  • 中国生物医学SinoMed
中国精品科技期刊2020
刘洋,韩忠明,刘琳玲,等. 灵芝孢子粉多糖对APAP肝损伤的保护作用研究[J]. 食品工业科技,2024,45(11):289−295. doi: 10.13386/j.issn1002-0306.2023040285.
引用本文: 刘洋,韩忠明,刘琳玲,等. 灵芝孢子粉多糖对APAP肝损伤的保护作用研究[J]. 食品工业科技,2024,45(11):289−295. doi: 10.13386/j.issn1002-0306.2023040285.
LIU Yang, HAN Zhongming, LIU Linling, et al. Protect Effects of Ganoderma lucidum Spore Polysaccharide on Liver Injury Caused by Acetaminophen[J]. Science and Technology of Food Industry, 2024, 45(11): 289−295. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023040285.
Citation: LIU Yang, HAN Zhongming, LIU Linling, et al. Protect Effects of Ganoderma lucidum Spore Polysaccharide on Liver Injury Caused by Acetaminophen[J]. Science and Technology of Food Industry, 2024, 45(11): 289−295. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023040285.

灵芝孢子粉多糖对APAP肝损伤的保护作用研究

Protect Effects of Ganoderma lucidum Spore Polysaccharide on Liver Injury Caused by Acetaminophen

  • 摘要: 目的:探讨灵芝孢子粉多糖(Ganoderma lucidum spore polysaccharide,GLSP)在对乙酰氨基酚(Acetaminophen,APAP)肝损伤中的保护作用。方法:将50只小鼠随机分为五组,分别为空白组、模型组、阳性药物组、GLSP低剂量组和GLSP高剂量组,每组10只,各组按相应剂量连续14 d预给药后,使用APAP造模、取材,测定肝脏指数、血清指标(谷草转氨酶(glutamic oxalacetic transaminase,AST)、谷丙转氨酶(glutamic-pyruvic transaminase,ALT))及肝组织匀浆指标(谷胱甘肽(glutathione,GSH)、丙二醛(malonaldehyde,MDA)、超氧化物歧化酶(superoxide dismutase,SOD)、Caspase-3BaxBcl-xl)。结果:与空白组相比,模型组中ALT、AST、MDA及促凋亡基因Caspase-3Bax均极显著升高(P<0.01),SOD、GSH含量极显著降低(P<0.01),HE染色结果显示APAP处理的小鼠肝脏出现典型的小叶中心性坏死;多糖剂量组与模型组相比ALT、AST极显著降低(P<0.01),多糖低剂量组SOD、GSH显著升高(P<0.05),MDA显著降低(P<0.05),在多糖高剂量组中,SOD、GSH极显著升高(P<0.01),MDA极显著降低(P<0.01),多糖组促凋亡基因Caspase-3Bax极显著降低(P<0.01),且肝组织病理学观察中可见肝组织坏死面积减小。结论:灵芝孢子粉多糖对APAP肝损伤能够起到预保护作用,其作用机制可能与提高肝脏的抗氧化能力和抑制凋亡的发生有关。

     

    Abstract: Objective: To investigate the protective effects of Ganoderma lucidum spore polysaccharide (GLSP) on liver injury caused by acetaminophen (APAP). Methods: Fifty mice were randomly divided into five groups: Blank group, model group, positive drug group, GLSP low-dose group and GLSP high-dose group, with 10 mice in each group. After each group was pre-administered according to the corresponding dose for 14 consecutive days, the liver index, serum indexes (glutamic oxalacetic transaminase (AST), glutamic-pyruvic transaminase (ALT)) and liver tissue plasma indexes (glutathione (GSH), malonaldehyde (MDA), superoxide dismutase (SOD), Caspase-3, Bax and Bcl-xl) were determined. Results: Compared with the blank group, ALT, AST, MDA and proapoptotic genes Caspase-3 and Bax were very significantly increased in the model group (P<0.01). The contents of SOD and GSH decreased very significantly (P<0.01), and HE staining showed that APAP treated mice showed typical lobular central necrosis of liver. Compared with model group, ALT and AST were very significantly decreased in polysaccharide dose group (P<0.01), SOD and GSH were significantly increased (P<0.05) and MDA was significantly decreased in polysaccharide low-dose group (P<0.05), SOD and GSH were very significantly increased (P<0.01) and MDA was very significantly decreased in polysaccharide high-dose group (P<0.01), and pro-apoptotic genes Caspase-3 and Bax were very significantly decreased in polysaccharide group (P<0.01), and the liver histopathological observation showed that the necrotic area of liver tissue decreased. Conclusion: Ganoderma lucidum spore polysaccharide can preprotect APAP liver injury, and its mechanism may be related to improving the antioxidant capacity of liver and inhibiting the occurrence of apoptosis.

     

/

返回文章
返回