• EI
  • Scopus
  • 食品科学与工程领域高质量科技期刊分级目录第一方阵T1
  • DOAJ
  • EBSCO
  • 北大核心期刊
  • 中国核心学术期刊RCCSE
  • JST China
  • FSTA
  • 中国精品科技期刊
  • 中国农业核心期刊
  • CA
  • WJCI
  • 中国科技核心期刊CSTPCD
  • 中国生物医学SinoMed
中国精品科技期刊2020
李惠琳,刘昊,李珏,等. 萝卜籽酶解制备萝卜硫素工艺优化及其体外消化研究[J]. 食品工业科技,2024,45(9):1−9. doi: 10.13386/j.issn1002-0306.2023050037.
引用本文: 李惠琳,刘昊,李珏,等. 萝卜籽酶解制备萝卜硫素工艺优化及其体外消化研究[J]. 食品工业科技,2024,45(9):1−9. doi: 10.13386/j.issn1002-0306.2023050037.
LI Huilin, LIU Hao, LI Jue, et al. Process Optimization and in Vitro Digestion Research of Raphanus sativus Seeds Sulforaphane Prepared by Enzymolysis Method[J]. Science and Technology of Food Industry, 2024, 45(9): 1−9. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023050037.
Citation: LI Huilin, LIU Hao, LI Jue, et al. Process Optimization and in Vitro Digestion Research of Raphanus sativus Seeds Sulforaphane Prepared by Enzymolysis Method[J]. Science and Technology of Food Industry, 2024, 45(9): 1−9. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023050037.

萝卜籽酶解制备萝卜硫素工艺优化及其体外消化研究

Process Optimization and in Vitro Digestion Research of Raphanus sativus Seeds Sulforaphane Prepared by Enzymolysis Method

  • 摘要: 为探究酶解法制备萝卜籽萝卜硫素的最佳工艺及其消化特性。采用红萝卜种子为原料,以萝卜硫素得率为指标,通过单因素实验和响应面优化试验得出最佳酶解工艺条件。进一步采用体外模拟胃肠消化模型,探究萝卜籽乙酸乙酯提取物萝卜硫素含量及其抗氧化活性的变化规律。得到萝卜籽制备萝卜硫素的最佳酶解条件为:酶解时间22 min,酶解温度40 ℃,VC添加量0.8 mg/g,在此最佳工艺条件下,萝卜硫素得率为2.11±0.02 mg/g,与预测值(2.14 mg/g)接近,相对误差为1.4%,酶解工艺切实可行。体外消化实验结果表明,胃肠消化液中萝卜硫素保留率分别为79.64%,76.22%;与未消化提取物比较,胃消化液中ABTS+、DPPH、OH三种自由基清除率均显著降低(P<0.05),分别为54.23%、33.90%、15.07%,而肠消化液与胃消化液中自由基清除率均无显著性差异(P>0.05)。本研究丰富了酶法制备萝卜硫素方面的研究,将为萝卜籽开发及高附加值利用提供科学依据。

     

    Abstract: The objective of this study was to investigate the optimal preparation process and digestive properties of sulforaphane from Raphanus sativus seeds prepared by enzymatic method. Single factor experiment and response surface methodology were employed to optimize the enzymolysis conditions for extracting sulforaphane from red Raphanus sativus seeds, with sulforaphane yield as index. Additionally, an in vitro simulated gastrointestinal digestion model was used to evaluate the changes in content and antioxidant activity of sulforaphane in ethyl acetate extracts of Raphanus sativus seeds. The results revealed that the optimal preparation conditions were as follows: The enzymolysis time was 22 minutes, the enzymolysis temperature was 40 ℃, the vitamin C concentration was 0.8 mg/g. Under these conditions, the sulforaphane yield was 2.11±0.02 mg/g, which closely matched the predicted value of 2.14 mg/g. The relative error for the enzymolysis process was 1.4%, demonstrating its feasibility. Furthermore, in vitro digestion simulation showed that the retention rate of sulforaphane was 79.64% and 76.22% in gastric and intestinal fluids, respectively. The free radical scavenging rates of ABTS+, DPPH and OH in gastric fluid reached 54.23%, 33.90% and 15.07%, respectively, and all the values had significant (P<0.05) reductions in comparison with that of the undigested extracts. However, no significant (P>0.05) difference in the three radical scavenging rates was observed in intestinal and gastric digestive fluids. This study enriched the research on the preparation of sulforaphane by enzymolysis method, and provided experimental basis for the development and high value-added utilization of Raphanus sativus seeds.

     

/

返回文章
返回