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中国精品科技期刊2020
刘华文,邓美晴,陈北燕. QuEChERS-超高效液相色谱-串联质谱法测定茶叶中47种农药残留[J]. 食品工业科技,2024,45(9):1−10. doi: 10.13386/j.issn1002-0306.2023050213.
引用本文: 刘华文,邓美晴,陈北燕. QuEChERS-超高效液相色谱-串联质谱法测定茶叶中47种农药残留[J]. 食品工业科技,2024,45(9):1−10. doi: 10.13386/j.issn1002-0306.2023050213.
LIU Huawen, DENG Meiqing, CHEN Beiyan. Determination of 47 Pesticide Residues in Tea by QuEChERS Method Combined with Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry[J]. Science and Technology of Food Industry, 2024, 45(9): 1−10. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023050213.
Citation: LIU Huawen, DENG Meiqing, CHEN Beiyan. Determination of 47 Pesticide Residues in Tea by QuEChERS Method Combined with Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry[J]. Science and Technology of Food Industry, 2024, 45(9): 1−10. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023050213.

QuEChERS-超高效液相色谱-串联质谱法测定茶叶中47种农药残留

Determination of 47 Pesticide Residues in Tea by QuEChERS Method Combined with Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

  • 摘要: 建立一种QuEChERS-超高效液相色谱-串联质谱法同时测定茶叶中47种农药残留的分析方法。样品经1%乙酸乙腈提取,QuEChERS方法净化,配有电喷雾离子源(ESI±)的超高效液相色谱-串联质谱(UPLC-MS/MS),在正负离子多反应监测(MRM)模式下同时测定,基质匹配外标法定量。结果表明:47种目标化合物在一定范围内线性关系良好,相关系数(r)为0.9971~0.9996,检出限为0.001~0.006 mg/kg,定量限为0.002~0.020 mg/kg,平均回收率为73.4%~114.3%,相对标准偏差(n=6)为0.3%~19.9%。该方法灵敏、准确、稳定,可满足茶叶中47种农药残留的检测要求。

     

    Abstract: An analysis method for simultaneous determination of 47 kinds of pesticide residues in tea was established by QuEChERS method combined with ultra performance liquid chromatography-tandem mass spectrometry. The samples were extracted by acetonitrile containing 1% acetic acid, and purified by QuEChERS method. 47 Pesticides were determined by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) with an electrospray ion source (ESI±) in positive and negative ion switching multiple reaction monitoring (MRM), and quantified by matrix-matched external standard method. Method validation revealed satisfactory linearities with correlation coefficients (r) between 0.9971 and 0.9996, and limits of detection were 0.001~0.006 mg/kg while the limits of quantitation were 0.002~0.020 mg/kg, the average recoveries at three spiked levels were in the range of 73.4%~114.3%, with relative standard deviations (n=6) of 0.3%~19.9%. The method is sensitive, accurate, stable, and can meet the requirements for determination of 47 pesticide residues in tea.

     

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