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中国精品科技期刊2020
肖丹虹,李萍,邓媛元,等. 基于响应面法优化双酶同步酶解的全豌豆乳的稳定性及营养特性研究[J]. 食品工业科技,2024,45(8):235−246. doi: 10.13386/j.issn1002-0306.2023060114.
引用本文: 肖丹虹,李萍,邓媛元,等. 基于响应面法优化双酶同步酶解的全豌豆乳的稳定性及营养特性研究[J]. 食品工业科技,2024,45(8):235−246. doi: 10.13386/j.issn1002-0306.2023060114.
XIAO Danhong, LI Ping, DENG Yuanyuan, et al. Study on the Stability and Nutritional Properties of Double Enzyme Simultaneous Enzymatic Hydrolysis of Whole Pea Milk Based on Response Surface Method[J]. Science and Technology of Food Industry, 2024, 45(8): 235−246. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023060114.
Citation: XIAO Danhong, LI Ping, DENG Yuanyuan, et al. Study on the Stability and Nutritional Properties of Double Enzyme Simultaneous Enzymatic Hydrolysis of Whole Pea Milk Based on Response Surface Method[J]. Science and Technology of Food Industry, 2024, 45(8): 235−246. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023060114.

基于响应面法优化双酶同步酶解的全豌豆乳的稳定性及营养特性研究

Study on the Stability and Nutritional Properties of Double Enzyme Simultaneous Enzymatic Hydrolysis of Whole Pea Milk Based on Response Surface Method

  • 摘要: 豌豆作为一种大宗豆类,具有营养价值高及低致敏性等特点,但全豌豆乳体系易絮凝失稳,限制其在食品中的应用。本文以脱皮豌豆为原料,采用高压均质耦合生物酶解(中温α-淀粉酶和纤维素酶)处理,通过离心沉淀率等指标,以及Box-Behnken设计优化酶解工艺条件,揭示最优工艺下全豌豆乳的稳定性和营养特性变化规律。结果表明,相较于传统过滤、单一酶解、双酶分步酶解等方法,中温α-淀粉酶和纤维素酶双酶同步酶解制备的全豌豆乳稳定性更优,最佳的酶解工艺参数为:中温α-淀粉酶:纤维素酶为4.5:5.5(酶活力比,U/g淀粉),添加总量为12 U/g,酶解时间为65 min,该条件下制得的全豌豆乳的离心沉淀率最低(27.70%),在不经过滤和不添加稳定剂的情况下,贮藏60 d内无明显的沉淀分层。与传统单体营养素复配工艺得到的豌豆乳相比,该优化工艺下制得的全豌豆乳稳定性显著提高,淀粉的消化程度降低了12.74%,蛋白质的消化程度提高了16.41%。研究结果为浓浆类的植物乳开发提供一定的理论指导。

     

    Abstract: Peas had high nutritional values and low allergenicity as a bulk pulse. However, whole pea milk was prone to flocculation instability, which limited its application in food. In this paper, peeling peas were used as raw material and treated with high pressure homogenization coupled biological double enzymatic hydrolysis (medium temperature α-amylase and cellulase), and the stability and nutritional characteristics of whole pea milk were revealed under the optimal process through centrifugation precipitation rate and Box-Behnken design. The results showed that the stability of whole pea milk prepared by simultaneous enzymatic hydrolysis of α-amylase and cellulase was better than the counterparts processed with traditional filtering, individual enzymatic hydrolysis, and step hydrolysis of double enzymes. The optimal parameters of simultaneous enzymatic hydrolysis was as follows: Medium temperature α-amylase:cellulase was 4.5:5.5 (enzyme activity ratio, U/g starch), with a total addition of 12 U/g and 65 minutes of enzymic hydrolysis. The centrifugal precipitation rate of whole pea milk processed under the optimal conditions was the lowest (27.70%), and no significant precipitation stratification during 60 days of storage without filtration and without stabilizer. Compared with the pea milk obtained by the traditional individual nutrients formulation, the whole pea milk prepared under the optimized simultaneous enzymatic hydrolysis showed significantly improved stability. The starch digestion extent showed a reduce of 12.74%, while the protein digestion extent was increased by 16.41%. The study provides the theoretical guidance for the development of concentrated plant based milk.

     

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