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中国精品科技期刊2020
蔡春梅,黄玉叶,李丽燕,等. 黄芩提取物抗UVA诱导NIH3T3细胞光老化的作用[J]. 食品工业科技,2024,45(12):319−325. doi: 10.13386/j.issn1002-0306.2023070036.
引用本文: 蔡春梅,黄玉叶,李丽燕,等. 黄芩提取物抗UVA诱导NIH3T3细胞光老化的作用[J]. 食品工业科技,2024,45(12):319−325. doi: 10.13386/j.issn1002-0306.2023070036.
CAI Chunmei, HUANG Yuye, LI Liyan, et al. Scutellaria baicalensis Extract Inhibits the UVA-induced Photoaging in NIH3T3 Cells[J]. Science and Technology of Food Industry, 2024, 45(12): 319−325. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023070036.
Citation: CAI Chunmei, HUANG Yuye, LI Liyan, et al. Scutellaria baicalensis Extract Inhibits the UVA-induced Photoaging in NIH3T3 Cells[J]. Science and Technology of Food Industry, 2024, 45(12): 319−325. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023070036.

黄芩提取物抗UVA诱导NIH3T3细胞光老化的作用

Scutellaria baicalensis Extract Inhibits the UVA-induced Photoaging in NIH3T3 Cells

  • 摘要: 目的:探究黄芩提取物对紫外线(UVA)诱导的成纤维细胞系(NIH3T3)光老化的保护作用。方法:通过UVA照射建立细胞光老化模型,采用CCK-8检测、β-半乳糖苷酶染色、DAPI染色、免疫荧光染色等技术,探讨黄芩提取物对光老化成纤维细胞的保护作用。结果:黄芩提取物能显著促进NIH3T3的细胞增殖与迁移(P<0.05),提高UVA照射后NIH3T3细胞存活率、降低UVA照射引起的细胞凋亡及衰老相关胞外β-半乳糖苷酶(SA-β-gal)的表达,并促进内源性I型、IV型胶原蛋白再生,逆转UVA照射引起的胶原蛋白流失,多方面共同作用抵御细胞光老化。结论:黄芩提取物能有效抑制因UVA照射引起的NIH3T3细胞光老化。

     

    Abstract: Objective: To investigate the protective effect of Scutellaria baicalensis extract on ultraviolet (UVA)-induced photoaging in a fibroblast cell line (NIH3T3). Methods: The cellular photoaging model was established by UVA irradiation, and the protective effects of Scutellaria baicalensis extract on photoaging fibroblasts were investigated using CCK-8 assay, β-galactosidase staining, DAPI staining, and immunofluorescence staining. Results: Scutellaria baicalensis extract significantly promoted cell proliferation and migration of NIH3T3 cells (P<0.05), increased the cell viability of NIH3T3 cells after UVA irradiation, reduced UVA-induced cell apoptosis, and the expression of senescence-associated marker β-galactosidase (SA-β-gal). It also promoted the regeneration of endogenous type I and type IV collagen, reversing collagen loss induced by UVA irradiation, collectively contributing to the defense against cellular photoaging. Conclusion: Scutellaria baicalensis extract effectively inhibits the photoaging of NIH3T3 cells induced by UVA irradiation.

     

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