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中国精品科技期刊2020
陈洁,陈睿然,郭福川. 铁过载对不同类型高脂膳食所致肝损伤的影响[J]. 食品工业科技,2024,45(12):326−333. doi: 10.13386/j.issn1002-0306.2023070067.
引用本文: 陈洁,陈睿然,郭福川. 铁过载对不同类型高脂膳食所致肝损伤的影响[J]. 食品工业科技,2024,45(12):326−333. doi: 10.13386/j.issn1002-0306.2023070067.
CHEN Jie, CHEN Ruiran, GUO Fuchuan. Effects of Iron Overload on Liver Injury caused by Different Types of High-fat Diets[J]. Science and Technology of Food Industry, 2024, 45(12): 326−333. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023070067.
Citation: CHEN Jie, CHEN Ruiran, GUO Fuchuan. Effects of Iron Overload on Liver Injury caused by Different Types of High-fat Diets[J]. Science and Technology of Food Industry, 2024, 45(12): 326−333. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023070067.

铁过载对不同类型高脂膳食所致肝损伤的影响

Effects of Iron Overload on Liver Injury caused by Different Types of High-fat Diets

  • 摘要: 目的:探究铁过载联合不同类型高脂膳食对小鼠肝损伤的影响。方法:将48只SPF级雄性C57BL/6J小鼠按体重随机分为6组,每组8只,普通对照组(ND)、高铁对照组(NDFe)给予基础饲料,棕榈油高脂组(PHFD)、棕榈油高脂高铁组(PHFDFe)、大豆油高脂组(SHFD)和大豆油高脂高铁组(SHFDFe)分别给予对应高脂饲料。从第10周开始,NDFe、PHFDFe和SHFDFe组连续8周每周两次肌肉注射右旋糖酐铁100 mg/kg·bw,其余组注射等剂量生理盐水至第17周。麻醉后取血和肝脏,测定小鼠血清和肝脏生化指标、肝脏病理改变及铁代谢和脂代谢相关基因表达。结果:与对应高脂组相比,铁过载联合高脂膳食可使血清总胆固醇(Total cholesterol,TC)、肝脏甘油三酯(Triglyceride,TG)和谷胱甘肽过氧化物酶(Glutathione peroxidase,GSH-Px)水平显著下降(P<0.05),血清TG和谷丙转氨酶(Alanine aminotransferase,ALT)水平、肝脏系数、肝脏铁含量和丙二醛(Malondialdehyde,MDA)水平显著升高(P<0.05),SHFDFe组肝脏MDA水平显著高于PHFDFe组(P<0.05)。PHFDFe组二价金属转运蛋白1(Divalentmetal-iontransporter-1,DMT-1)和膜铁转运蛋白(Ferroportin,FPN) mRNA表达量显著高于PHFD组(P<0.05);SHFDFe组FPN mRNA表达量显著高于与NDFe、PHFDFe和SHFD组(P<0.05),乙酰CoA羧化酶1(acetyl-Coenzyme A carboxylase alpha 1,ACC1)和脂肪酸合酶(Fatty Acid Synthase,FASN) mRNA表达量显著低于SHFD组(P<0.05)。结论:铁过载联合高脂膳食会加重脂质代谢紊乱和氧化应激,铁过载联合大豆油高脂饲料喂养导致的肝损伤高于联合棕榈油高脂饲料喂养。

     

    Abstract: Objective: To investigate the effects of iron overload in conjunction with different types of high-fat diets on liver injury in mice. Methods: Forty-eight SPF male C57BL/6J mice were randomly divided into six groups based on their body weight (n=8 per group). The normal control group (ND) and normal diet with high iron group (NDFe) were fed with basal diet. The palm oil high-fat diet group (PHFD), palm oil high-fat diet with high iron group (PHFDFe), soybean oil high-fat diet group (SHFD), and soybean oil high-fat diet with high iron group (SHFDFe) were fed with their respective high-fat diets. Starting from the 10th week, mice in the NDFe, PHFDFe, and SHFDFe groups were administered iron dextran through intramuscular injection at a dosage of 100 mg/kg·bw twice a week for 8 consecutive weeks. Meanwhile, the remaining groups were given saline injections of an equal dose until the 17th week. Blood and liver samples were collected after anesthesia to evaluate serum and liver biochemical indexes. Additionally, liver pathological changes were examined, and the expression of genes associated with iron and lipid metabolism was analyzed. Results: Compared with the respective high-fat groups, iron overload in conjunction with high-fat diet significantly decreased serum total cholesterol (TC), hepatic triglyceride (TG) and glutathione peroxidase (GSH-Px) levels while increased serum TG and alanine aminotransferase (ALT), liver index, liver iron and malondialdehyde (MDA) levels (P<0.05). Additionally, liver MDA content of the SHFDFe group was significantly higher than that of the PHFDFe group (P<0.05). Compared with the PHFD group, the mRNA expression levels of Divalentmetal-iontransporter-1 (DMT-1) and Ferroportin (FPN) were significantly increased in the PHFDFe group (P<0.05). FPN mRNA expression level was significantly upregulated in the SHFDFe group when compared with the NDFe, PHFDFe and SHFD groups (P<0.05). Moreover, the mRNA expression levels of acetyl-Coenzyme A carboxylase alpha 1 (ACC1) and Fatty Acid Synthase (FASN) were significantly decreased in the SHFDFe group compared to the SHFD group (P<0.05). Conclusion: The combination of iron overload and high-fat diets has the potential to worsen lipid metabolism disorder and oxidative stress. Additionally, when combined with iron overload, soybean oil may cause more severe liver injury compared to palm oil.

     

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