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中国精品科技期刊2020
孙仪征,王慧琴,李贝贝. 六堡砖茶多糖制备及其免疫调节活性[J]. 食品工业科技,2024,45(14):327−334. doi: 10.13386/j.issn1002-0306.2023070068.
引用本文: 孙仪征,王慧琴,李贝贝. 六堡砖茶多糖制备及其免疫调节活性[J]. 食品工业科技,2024,45(14):327−334. doi: 10.13386/j.issn1002-0306.2023070068.
SUN Yizheng, WANG Huiqin, LI Beibei. Preparation of Polysaccharides from Liubao Brick Tea and Its Immunomodulatory Activity[J]. Science and Technology of Food Industry, 2024, 45(14): 327−334. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023070068.
Citation: SUN Yizheng, WANG Huiqin, LI Beibei. Preparation of Polysaccharides from Liubao Brick Tea and Its Immunomodulatory Activity[J]. Science and Technology of Food Industry, 2024, 45(14): 327−334. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023070068.

六堡砖茶多糖制备及其免疫调节活性

Preparation of Polysaccharides from Liubao Brick Tea and Its Immunomodulatory Activity

  • 摘要: 目的:优化六堡砖茶多糖提取工艺,并进一步研究其免疫调节作用及其可能的调节机制。方法:采用单因素实验探究提取温度、料液比及乙醇浓度对茶多糖得率的影响,在此基础上与响应面试验相结合,对茶多糖提取工艺进行了优化。通过测定六堡砖茶多糖对RAW264.7细胞的吞噬能力及NO释放量,检测核因子κB(Nuclear factor-κB,NF-κB)和AMP依赖的蛋白激酶(AMP-dependent protein kinase,AMPK)信号通路相关基因及蛋白表达,评价纯化后的茶多糖的体外免疫调节活性。结果:六堡砖茶多糖的最佳提取工艺为:提取温度91.11 ℃,料液比1:15.45(g/mL),乙醇浓度82.84%(v/v)。在此条件下,茶多糖得率为8.62%。细胞实验显示六堡砖茶多糖可显著增强RAW264.7细胞的吞噬能力及NO释放量(P<0.05),显著激活NF-κB和AMPK信号通路(P<0.05),显著上调诱导型一氧化氮合成酶(Inducible nitric oxide synthase,iNOS)、肿瘤坏死因子-α(Tumor necrosis factor-α,TNF-α)、白细胞介素-6(Interleukin-6,IL-6)、白细胞介素-1β(Interleukin-1β,IL-1β)的mRNA表达水平(P<0.05)。其中,高剂量茶多糖效果更佳。结论:通过优化提取温度、料液比及乙醇浓度条件,可有效提高六堡砖茶多糖得率,并可通过激活NF-кB/AMPK通路发挥免疫调节作用。该研究结果为六堡砖茶多糖活性深入探究和应用提供了研究基础。

     

    Abstract: Objective: To optimize the extraction process of tea polysaccharides from Liubao brick tea and to further investigate the immunomodulatory effects and the possible regulatory mechanisms. Methods: A single-factor experiment was used to investigate the effects of extraction temperature, material-liquid ratio, and ethanol concentration on the yield of tea polysaccharides, based on which the extraction process was optimized by combining with response surface methodology. By measuring the phagocytosis and nitric oxide (NO) release of Liubao brick tea polysaccharides on RAW264.7 cells, detecting the expression of genes and proteins related to nuclear factor-κB (NF-κB) and AMP-dependent protein kinase (AMPK) signaling pathway, in vitro immunomodulatory activity of the purified tea polysaccharides was evaluated. Results: The optimal extraction process for the polysaccharides from Liubao brick tea were as follows: 91.11 ℃ of the extraction temperature, 1:15.45 of the solid-liquid ratio, and 82.84% of the ethanol concentration. In vitro immunomodulatory activity assay showed that Liubao brick tea polysaccharides significantly enhanced phagocytosis and NO release of RAW264.7 cells, significantly activated NF-κB and AMPK signaling pathway, and significantly up-regulated the mRNA expression levels of inducible nitric oxide synthase (iNOS), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β) to exert immunomodulatory effects. Conclusion: By optimizing the extraction temperature, material-liquid ratio and ethanol concentration, the yield of Liubao brick tea polysaccharide could be effectively improved, and it could play an immunomodulatory role by activating the NF-кB/AMPK pathway. The results provide a research basis for the in-depth investigation and application of the Liubao brick polysaccharide.

     

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