• EI
  • Scopus
  • 食品科学与工程领域高质量科技期刊分级目录第一方阵T1
  • DOAJ
  • EBSCO
  • 北大核心期刊
  • 中国核心学术期刊RCCSE
  • JST China
  • FSTA
  • 中国精品科技期刊
  • 中国农业核心期刊
  • CA
  • WJCI
  • 中国科技核心期刊CSTPCD
  • 中国生物医学SinoMed
中国精品科技期刊2020
王鑫,周卓,遇世友,等. 甜玉米芯多糖纳米银的合成、表征及体外活性研究[J]. 食品工业科技,2024,45(13):58−66. doi: 10.13386/j.issn1002-0306.2023070111.
引用本文: 王鑫,周卓,遇世友,等. 甜玉米芯多糖纳米银的合成、表征及体外活性研究[J]. 食品工业科技,2024,45(13):58−66. doi: 10.13386/j.issn1002-0306.2023070111.
WANG Xin, ZHOU Zhuo, YU Shiyou, et al. Study on Synthesis, Characterization and in Vitro Activity of Sweet Corncob Polysaccharide Nano-Silver[J]. Science and Technology of Food Industry, 2024, 45(13): 58−66. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023070111.
Citation: WANG Xin, ZHOU Zhuo, YU Shiyou, et al. Study on Synthesis, Characterization and in Vitro Activity of Sweet Corncob Polysaccharide Nano-Silver[J]. Science and Technology of Food Industry, 2024, 45(13): 58−66. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023070111.

甜玉米芯多糖纳米银的合成、表征及体外活性研究

Study on Synthesis, Characterization and in Vitro Activity of Sweet Corncob Polysaccharide Nano-Silver

  • 摘要: 纳米银(AgNPs)在生物医药领域有广泛应用,但传统物理化学方法合成纳米银存在污染及产物毒性、耗时长等问题,因此探究纳米银的制备极其重要。以甜玉米芯多糖(SCP)为原料,采用绿色方法成功合成了甜玉米芯多糖纳米银(SCP-AgNPs)并对反应条件进行优化,使用透射电子显微镜(TEM)、X射线衍射仪、X光电子能谱仪、傅里叶变换红外光谱对生成的纳米银进行表征,并对其体外抗氧化及抗糖尿病能力进行探究。结果表明:SCP-AgNPs的最佳反应条件为:溶液中硝酸银浓度为0.75×10−3 mol/L,pH为10.0,反应80 min,所制备的SCP-AgNPs呈球状,平均粒径为13.5 nm。结构表现为面心立方晶体结构,确定其中Ag为Ag0状态。合成的SCP-AgNPs有较强的体外清除自由基作用。同时,SCP-AgNPs对α-葡萄糖苷酶和α-淀粉酶这两种糖尿病标记酶的活性具有良好的抑制作用。SCP-AgNPs对糖尿病关键酶具有显著的抑制效果,具备潜在抗糖尿病活性,是一种适合纳米生物医学应用的纳米药物,未来将广泛应用于食品加工、医学等领域。

     

    Abstract: Silver nanoparticles (AgNPs) are widely used in the field of biomedicine. However, the synthesis of AgNPs by traditional physical and chemical methods has problems such as pollution, product toxicity, and time-consuming. Therefore, it is extremely important to explore the preparation of AgNPs. Silver nanoparticles (AgNPs) of sweet corncob polysaccharide (SCP-AgNPs) were successfully synthesized by green method using sweet corncob polysaccharide (SCP) as raw material and the reaction conditions were optimized. The generated AgNPs were characterized by transmission electron microscopy (TEM), X-ray diffraction, X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy, and their antioxidant and anti-diabetic abilities in vitro were investigated. The results showed that the optimum reaction conditions of SCP-AgNPs were as follows: The concentration of silver nitrate in the solution was 0.75×10−3 mol/L, the pH was 10.0, and the reaction time was 80 min. The prepared SCP-AgNPs were spherical with an average particle size of 13.5 nm. The structure showed a face-centered cubic crystal structure, and Ag was determined to be Ag0 state. The synthesized SCP-AgNPs had strong free radical scavenging effect in vitro. At the same time, SCP-AgNPs had a good inhibitory effect on the activity of α-glucosidase and α-amylase. SCP-AgNPs has a significant inhibitory effect on key enzymes of diabetes and has potential anti-diabetic activity. It is a nano-drug suitable for nano-biomedical applications and will be widely used in food processing, medicine and other fields in the future.

     

/

返回文章
返回