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中国精品科技期刊2020
祁兴普,王俊凯,邹婷婷,等. 基于磁性纳米粒子的比色适配体传感器检测赭曲霉毒素A[J]. 食品工业科技,2024,45(11):253−259. doi: 10.13386/j.issn1002-0306.2023080021.
引用本文: 祁兴普,王俊凯,邹婷婷,等. 基于磁性纳米粒子的比色适配体传感器检测赭曲霉毒素A[J]. 食品工业科技,2024,45(11):253−259. doi: 10.13386/j.issn1002-0306.2023080021.
QI Xingpu, WANG Junkai, ZOU Tingting, et al. A Colorimetric Aptamer Sensor Based on Magnetic Nanoparticle for the Detection of Ochratoxin A[J]. Science and Technology of Food Industry, 2024, 45(11): 253−259. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023080021.
Citation: QI Xingpu, WANG Junkai, ZOU Tingting, et al. A Colorimetric Aptamer Sensor Based on Magnetic Nanoparticle for the Detection of Ochratoxin A[J]. Science and Technology of Food Industry, 2024, 45(11): 253−259. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023080021.

基于磁性纳米粒子的比色适配体传感器检测赭曲霉毒素A

A Colorimetric Aptamer Sensor Based on Magnetic Nanoparticle for the Detection of Ochratoxin A

  • 摘要: 基于磁性纳米粒子(Fe3O4@Au)和纳米银(Ag NPs),通过DNA碱基互补配对,构建了一种新颖的比率比色传感器用于赭曲霉毒素A(Ochratoxin A,OTA)的检测。OTA适配体(aptamer)偶联的Fe3O4@Au作为参比信号,互补DNA(cDNA)偶联的Ag NPs作为响应信号,通过调控两者的比例以及DNA碱基互补,制备了具有两个紫外吸收峰的比率纳米探针(Fe3O4@Au-Ag)。没有OTA存在时,比率比色传感器的吸光度比值(A400/A580)保持恒定。对检测条件(时间、pH和温度)进行了优化,在优化条件下,该传感器对OTA检测呈现良好的线性关系,线性范围为0.01~1000 ng·mL−1,检出限为0.033 ng·mL−1。该传感器具有良好的选择性、重现性和高灵敏度,并被成功应用于豌豆粉中OTA的检测,回收率为93.9%~96.0%。

     

    Abstract: A novel ratio colorimetric sensor based on magnetic nanoparticles (Fe3O4@Au) and silver nanoparticles (Ag NPs) was constructed for the detection of Ochratoxin A (OTA) by complementary DNA base pairing. The OTA aptamer coupled Fe3O4@Au was served as reference signal, and complementary DNA (cDNA) coupled Ag NPs was served as the response signal. A ratio nanoprobe (Fe3O4@Au-Ag) with two UV absorption peaks was prepared by regulating the ratio and DNA base complementarity. In absence of OTA, the absorbance peak ratio (A400/A580) of the ratio colorimetric sensor could maintain a constant value. The detection conditions (time, pH, and temperature) were optimized, and under optimized conditions, the sensor showed a good linear relationship to OTA detection, with a linear range of 0.01 ng·mL−1 to 1000 ng·mL−1 and a detection limit of 0.033 ng·mL−1. The sensor had good selectivity, reproducibility and high sensitivity, which was successfully applied to the detection of OTA in pea powder with a recovery rate of 93.9%~96.0%.

     

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