• EI
  • Scopus
  • 食品科学与工程领域高质量科技期刊分级目录第一方阵T1
  • DOAJ
  • EBSCO
  • 北大核心期刊
  • 中国核心学术期刊RCCSE
  • JST China
  • FSTA
  • 中国精品科技期刊
  • 中国农业核心期刊
  • CA
  • WJCI
  • 中国科技核心期刊CSTPCD
  • 中国生物医学SinoMed
中国精品科技期刊2020
刘梦文,沈静,阿依努尔·白克热,等. 一测多评法同时测定桑叶中9种绿原酸类和黄酮类成分[J]. 食品工业科技,xxxx,x(x):1−9. doi: 10.13386/j.issn1002-0306.2023080195.
引用本文: 刘梦文,沈静,阿依努尔·白克热,等. 一测多评法同时测定桑叶中9种绿原酸类和黄酮类成分[J]. 食品工业科技,xxxx,x(x):1−9. doi: 10.13386/j.issn1002-0306.2023080195.
LIU Mengwen, SHEN Jing, AYINUER·Baikere , et al. Simultaneous Determination of Nine Chlorogenic Acids and Flavonoids in Mori Folium by QAMS[J]. Science and Technology of Food Industry, xxxx, x(x): 1−9. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023080195.
Citation: LIU Mengwen, SHEN Jing, AYINUER·Baikere , et al. Simultaneous Determination of Nine Chlorogenic Acids and Flavonoids in Mori Folium by QAMS[J]. Science and Technology of Food Industry, xxxx, x(x): 1−9. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023080195.

一测多评法同时测定桑叶中9种绿原酸类和黄酮类成分

Simultaneous Determination of Nine Chlorogenic Acids and Flavonoids in Mori Folium by QAMS

  • 摘要: 目的:建立一测多评法同时测定桑叶中新绿原酸、绿原酸、隐绿原酸、芦丁、异槲皮苷、异绿原酸B、紫云英苷、异绿原酸A和异绿原酸C的含量。方法:采用Agilent TC-C18色谱柱(250 mm×4.6 mm,5 μm),以乙腈(A)-0.1%磷酸水溶液(B)为流动相,梯度洗脱,流速1.0 mL/min,检测波长260、320 nm,柱温30 ℃。以绿原酸为内参物,分别采用外标法和一测多评(多点校正、斜率校正和单点校正)测定桑叶中9个成分的含量并比较4种方法之间的差异,另外采用多点校正和两点校正法进行色谱峰定位,验证该方法的准确性和可行性。结果:新绿原酸、隐绿原酸、芦丁、异槲皮苷、异绿原酸B、紫云英苷、异绿原酸A和异绿原酸C相对于绿原酸的校正因子分别为0.9072、0.8736、0.6207、0.8547、1.1936、0.5501、1.4369和1.2244(多点校正),且在不同条件下相对校正因子耐用性(RSD<1.5%)和重现性(RSD<5%)良好。外标法和一测多评(多点校正、斜率校正和单点校正)测得桑叶中9个成分含量的结果之间无差异(RSD<1.5%)。相较于相对保留时间,多点校正和两点校正可改善色谱峰的准确定位(相对误差|RE|<3%)。结论:建立了桑叶中9种成分的一测多评法,该法准确可行,可用于桑叶的质量控制。

     

    Abstract: Objective: To establish a HPLC method for the determination of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, rutin, isoquercitrin, isochlorogenic acid B, astragalin, isochlorogenic acid A and isochlorogenic acid C in Mori Folium using the quantitative analysis of multi-components by single marker (QAMS). Methods: The analysis was performed on an Agilent TC-C18 column (250 mm×4.6 mm, 5 μm), with acetonitrile (A)-0.1% phosphoric acid (B) as mobile phase at the flow rate of 1.0 mL/min for gradient elution, as well as the wavelengths were 260 and 320 nm and the column temperature was 30 ℃. The chlorogenic acid was used as the internal reference. The contents of nine components in Mori Folium were calculated by the external standard method and QAMS (multi-point correction, gradient correction and single-point correction) respectively, and the differences among the four methods were compared. In addition, the accuracy and feasibility of the method were verified by using multi-point correction and two-point correction to locate chromatographic peaks of the components. Results: The relative correction factors of neochlorogenic acid, cryptochlorogenic acid, rutin, isoquercitrin, isochlorogenic acid B, asiaticoside, isochlorogenic acid A and isochlorogenic acid C to chlorogenic acid were 0.9072, 0.8736, 0.6207, 0.8547, 1.1936, 0.5501, 1.4369 and 1.2244, respectively (multi-point correction). The durability (RSD<1.5%) and the reproducibility (RSD<5%) of the relative correction factors were positive under different conditions. The content of nine components in Mori Folium was determined simultaneously by the external standard method and QAMS (multi-point correction, gradient correction and single-point correction), and there was no difference between the results obtained from the four calculation methods (RSD<1.5%). Compared to the relative retention time, multi-point correction and two-point correction could improve the accurate localization of the chromatographic peaks (relative error |RE|<3%). Conclusion: The method of QAMS for nine components determination in Mori Folium was established, which is accurate and feasible and can be applied for the quality control of Mori Folium.

     

/

返回文章
返回