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中国精品科技期刊2020
孟庆霖,褚齐,宋健,等. 紫苏叶总黄酮对APAP诱导的急性肝损伤的保护作用[J]. 食品工业科技,2024,45(14):1−8. doi: 10.13386/j.issn1002-0306.2023080238.
引用本文: 孟庆霖,褚齐,宋健,等. 紫苏叶总黄酮对APAP诱导的急性肝损伤的保护作用[J]. 食品工业科技,2024,45(14):1−8. doi: 10.13386/j.issn1002-0306.2023080238.
MENG Qinglin, CHU Qi, SONG Jian, et al. The Protective Effect of Total Favonoids of Perilla frutescens on APAP-Induced Acute Liver Injury[J]. Science and Technology of Food Industry, 2024, 45(14): 1−8. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023080238.
Citation: MENG Qinglin, CHU Qi, SONG Jian, et al. The Protective Effect of Total Favonoids of Perilla frutescens on APAP-Induced Acute Liver Injury[J]. Science and Technology of Food Industry, 2024, 45(14): 1−8. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023080238.

紫苏叶总黄酮对APAP诱导的急性肝损伤的保护作用

The Protective Effect of Total Favonoids of Perilla frutescens on APAP-Induced Acute Liver Injury

  • 摘要: 目的:本文主要研究紫苏叶总黄酮(Perilla flavone,PF)纯化的最佳方法,以及其对对乙酰氨基酚(Paracetamol,APAP)诱导的急性肝损伤的保护作用及可能的作用机制。方法:采用单因素实验确定大孔树脂纯化紫苏叶总黄酮的最佳条件。将BALB/c雄性小鼠随机分为6组,空白对照组(CON)、模型组(MOD)、联苯双酯组(DDB)、PF低剂量组(L-PF,12.5 mg/kg)、PF中剂量组(M-PF,25 mg/kg)、PF高剂量组(H-PF,50 mg/kg),连续灌胃给药15 d。末次给药1 h后,除CON组外,每组腹腔注射APAP建立肝损伤模型。测定小鼠血清中谷草转氨酶(AST)、谷丙转氨酶(ALT)水平;测定小鼠肝脏中丙二醛(MDA)、超氧化物歧物酶(SOD)、还原型谷胱甘肽(GSH)、谷胱甘肽过氧化物酶(GSH-PX)的水平;对小鼠肝脏进行HE染色观察病理学变化;Western blot法检测小鼠肝脏中Nrf2、Keap1和HO-1蛋白表达。结果:最佳纯化条件:D101树脂10 g,上样溶液浓度为2.0 mg/mL,上样体积为30 mL,50 mL的80%乙醇溶液洗脱,纯化后黄酮含量为70.32%±1.49%。与CON组比较,MOD组小鼠血清中AST、ALT表达显著升高(P<0.01),说明肝损伤造模成功。与MOD组比较,给予PF组小鼠肝脏指数、AST、ALT、MDA、GSH水平和Keap 1蛋白表达显著降低25%~60%(P<0.01或P<0.05),SOD、GSH-PX水平和Nrf2、HO-1蛋白表达水平显著升高15%~35%(P<0.01或P<0.05)。HE染色结果显示PF可有效改善APAP引起的肝损伤。结论:紫苏叶总黄酮对APAP诱导的急性肝损伤有一定的保护作用,其机制与调控氧化应激的Nrf2/HO-1信号通路相关。

     

    Abstract: Objective: To study the best purification method of Perilla flavone (PF), and to investigate its protective effect and potential mechanism on Paracetamol (APAP) - induced acute liver injury. Methods: Single factor experiment was used to determine the optimal conditions for the purification of PF by macroporous resin. BALB/c male mice were randomly divided into 6 groups, blank control group (CON), model group (MOD), diphenylene dibenzoate group (DDB), low dose group of PF (L-PF, 12.5 mg/kg), middle dose group of PF (M-PF, 25 mg/kg), and high dose group of PF (H-PF, 50 mg/kg). DDB and PF were given by continuous intragastric administration for 15 days according to the dosage. After the last administration for 1 h, APAP was injected intraperitoneally to establish liver injury model in each group except CON group. The serum levels of AST and ALT of mice were determined, and the levels of MDA, SOD, GSH, and GSH-PX in the liver of mice were tested. HE staining of the livers was performed to observe pathological changes. The expressions of Nrf2, Keap1 and HO-1 proteins in the livers were detected. Results: The optimal purification conditions were as follows: 10 g of D101 resin, 2.0 mg/mL of up-sampling solution, up-sampling volume of 30 mL, 50 mL of 80% ethanol solution elution. After purification, the flavonoid content was 70.32%±1.49%. Compared with CON group, the expressions of AST and ALT in the serum were significantly higher in MOD group (P<0.01), indicating successful modeling of liver injury. Compared with MOD group, the liver index, AST, ALT, MDA, GSH levels and Keap 1 protein expression in PF-given groups were significantly reduced by 25%~60% (P<0.01 or P<0.05), the concentrations of SOD, GSH-PX, and the expression levels of Nrf2 and HO-1 protein were significantly increased by 15%~35% (P<0.01 or P<0.05). HE staining results showed that PF could significantly improve APAP-induced liver injury. Conclusion: The PF can protect APAP-induced acute liver injury, and its mechanism is related to the Nrf2/HO-1 signaling pathway which regulates oxidative stress.

     

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