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中国精品科技期刊2020
罗婧文,张欣,王辉,等. 刺梨多酚提取物对高脂饮食诱导的肥胖小鼠的改善作用[J]. 食品工业科技,2024,45(14):1−9. doi: 10.13386/j.issn1002-0306.2023110015.
引用本文: 罗婧文,张欣,王辉,等. 刺梨多酚提取物对高脂饮食诱导的肥胖小鼠的改善作用[J]. 食品工业科技,2024,45(14):1−9. doi: 10.13386/j.issn1002-0306.2023110015.
LUO Jingwen, ZHANG Xin, WANG Hui, et al. Ameliorating Effect of Polyphenol Extract of Rosa roxburghii on Obese Mice Induced by High Fat Diet[J]. Science and Technology of Food Industry, 2024, 45(14): 1−9. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023110015.
Citation: LUO Jingwen, ZHANG Xin, WANG Hui, et al. Ameliorating Effect of Polyphenol Extract of Rosa roxburghii on Obese Mice Induced by High Fat Diet[J]. Science and Technology of Food Industry, 2024, 45(14): 1−9. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023110015.

刺梨多酚提取物对高脂饮食诱导的肥胖小鼠的改善作用

Ameliorating Effect of Polyphenol Extract of Rosa roxburghii on Obese Mice Induced by High Fat Diet

  • 摘要: 目的:为了研究刺梨多酚提取物对高脂饮食诱导的肥胖小鼠的改善作用。方法:分别以多酚含量400 mg/kg BW/d的剂量对高脂饮食小鼠进行干预。连续灌胃4周,测定小鼠体重、肝脏指数、白色脂肪及肝脏组织形态学变化、血清生化指标、脏器抗氧化能力和肠道屏障。结果:与CON组相比,MODEL组胰岛素水平(Insulln,INS)和炎症因子水平均显著提高,血脂四项中高密度脂肪(High-density lipoproteincholesterol,HDL-C)水平提高,总胆固醇(Total cholesterol,TC)、甘油三酯(Triglyceride,TG)和低密度脂肪(Low-density lipoproteincholesterol,LDL-C)水平降低;抗氧化物中超氧化物歧化酶(Superoxide Dismutase,SOD)和谷胱甘肽过氧化物酶(Glutathione peroxidase,GPx)活力下降,肠道屏障蛋白表达水平及短链脂肪酸(Short-chain fatty acids,SCFAs)水平均显著降低(P<0.05);表明造模成功,肥胖小鼠体内代谢紊乱。与MODEL相比,多酚干预组(RF、RP组)炎症因子和氧化应激水平降低,抗氧化物SOD、GPx活力提升,肠道内ZO-1、Claduin-1和SCFAs水平均发生逆转(P<0.05)。结论:刺梨多酚提取物能有效缓解高脂饮食诱导的肥胖小鼠体内代谢紊乱,减缓体重增长,保护脂肪和肝脏组织细胞形态变化,缓解肥胖小鼠体内炎症和氧化应激水平,增强肠道屏障功能,且RF效果优于RP。

     

    Abstract: Objective: This study aimed to investigate the beneficial effects of phenolic extracts of Rosa roxburghii Tratt on obese mice induced by a high-fat diet. Methods: The intervention involved administering polyphenol extract at a dose of 400 mg/kg body weight per day to mice on a high-fat diet. The mice were orally gavaged continuously for 4 weeks, and various parameters such as body weight, liver index, histomorphological changes in white fat and liver tissues, serum biochemical markers, organ antioxidant capacity, and intestinal barrier function were examined. Results: In comparison to the control group (CON), the MODEL group (MODEL) exhibited significantly increased insulin levels, inflammatory factors, and high-density lipoprotein cholesterol (HDL-C) levels. Total cholesterol (TC) and triglyceride (TG) levels decreased in the MODEL group, along with reduced activity of antioxidant enzymes superoxide dismutase (SOD) and glutathione peroxidase (GPx). The expression of intestinal barrier proteins and levels of short-chain fatty acids (SCFAs) were also significantly reduced, indicating successful modeling of obesity with disturbed metabolism in mice. In comparison to the MODEL group, the polyphenol intervention groups (RF and RP) showed decreased levels of inflammatory factors and oxidative stress, increased antioxidant enzyme activity, and restoration of intestinal barrier proteins and SCFAs. Conclusion: Prickly pear polyphenol extract effectively alleviated metabolic disorders in high-fat diet-induced obese mice in vivo, resulting in reduced body weight gain, protection against cellular changes in adipose and hepatic tissues, alleviation of inflammation and oxidative stress, and enhanced intestinal barrier function. Notably, the RF group showed a superior effect to the RP group.

     

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