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中国精品科技期刊2020
姜秀杰,张家瑜,李莹,等. 富含γ-氨基丁酸的萌发红小豆对T2DM小鼠肠道菌群的影响[J]. 食品工业科技,2024,45(12):152−160. doi: 10.13386/j.issn1002-0306.2023120301.
引用本文: 姜秀杰,张家瑜,李莹,等. 富含γ-氨基丁酸的萌发红小豆对T2DM小鼠肠道菌群的影响[J]. 食品工业科技,2024,45(12):152−160. doi: 10.13386/j.issn1002-0306.2023120301.
JIANG Xiujie, ZHANG Jiayu, LI Ying, et al. Effect of Rich GABA of Germinated Adzuki Bean on Intestinal Microflora in T2DM Mice[J]. Science and Technology of Food Industry, 2024, 45(12): 152−160. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023120301.
Citation: JIANG Xiujie, ZHANG Jiayu, LI Ying, et al. Effect of Rich GABA of Germinated Adzuki Bean on Intestinal Microflora in T2DM Mice[J]. Science and Technology of Food Industry, 2024, 45(12): 152−160. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023120301.

富含γ-氨基丁酸的萌发红小豆对T2DM小鼠肠道菌群的影响

Effect of Rich GABA of Germinated Adzuki Bean on Intestinal Microflora in T2DM Mice

  • 摘要: 为了研究富含GABA萌发红小豆对2型糖尿病(Diabetes Mellitus Type 2, T2DM)小鼠血糖水平及肠道菌群的影响,采用C57BL/6J小鼠为研究对象,通过高脂膳食+链脲菌素(Streptozocin, STZ)注射构建T2DM模型,选择不同剂量的富含GABA萌发红小豆对T2DM小鼠连续膳食干预6周,并利用16S rRNA测序技术对T2DM小鼠盲肠内容物的菌群结构和分布进行鉴定。结果显示:不同剂量富含GABA红小豆膳食干预可使T2DM小鼠FBG值明显下降,其中高剂量富含GABA红小豆(TF3)组FBG值为8.36±0.78 mmol/L,相比模型组(M)下降54.09%,干预效果最好。此外,TF3膳食可引起T2DM小鼠肠道菌群丰度发生显著(P<0.05)改变,门水平上Firmicutes丰度为35.96%,比M模型组下降53.17%,并可显著上调Bacteroidetes、Verrucomicrobia的菌群丰度(P<0.05)。表明TF3膳食改善糖脂代谢与Bacteroidetes、Verrucomicrobia优势菌丰度呈正相关,暗示高剂量富含GABA红小豆膳食可通过增加有益菌来缓解T2DM小鼠高血糖症状,为进一步解释T2DM与肠道菌群的关系提供参考。

     

    Abstract: In order to study the effects of GABA-rich germinated adzuki bean on blood glucose level and intestinal flora in T2DM mice. C57BL/6J mice were used as subjects to construct T2DM model by high fat diet+STZ injection. Different doses of GABA-rich germinated adzuki bean were used to treat T2DM mice for 6 weeks and 16S rRNA sequencing technique was used to identify the microbial structure and distribution in the cecal contents of T2DM mice. The results showed that different doses of GABA-rich adzuki bean dietary intervention could significantly reduce the FBG value of T2DM mice, and the FBG value of high dose GABA-rich adzuki bean (TF3) group was 8.36±0.78 mmol/L, which was 54.09%, lower than that of model group (M). In addition, TF3 diet induced significant changes in intestinal flora abundance in T2DM mice. Firmicutes abundance at gate level was 35.96%, which decreased by 53.17% compared with M model group, and Bacteroidetes and Verrucomicrobia abundance were significantly up-regulated. In summary, the improvement of glucose and lipid metabolism by TF3 diet was significantly correlated with the abundance of dominant bacteria in Bacteroidetes and Verrucomicrobia, suggesting that high dose of GABA-rich adzuki bean diet could alleviate hyperglycemia symptoms in T2DM mice through beneficial bacteria, which provided reference for further explaining the relationship between T2DM and intestinal flora.

     

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