Abstract: Objective: With Lycium barbarum leaves flavonoids (LBLF) as the raw material, a kind of tablet candy (TC-LBLF) was prepared, its quality and functional properties were analyzed, and its effects on the high fructose (HF)-induced liver injury in mice were investigated. Methods: The formula of the tablet candy was determined through single factor and response surface experiments. A chronic liver injury model in mice was established by continuously feeding them with 30% HF of drinking water for four weeks. Liver-injured mice were randomly divided into two groups, model group (MD group) and TC-LBLF group (P group) (300 mg/kg∙bw), and after intervention for eight weeks, the organ index was calculated. The levels of insulin, total cholesterol (TC), triglycerides (TG), alanine aminotransferase (ALT), aspartate aminotransferase (AST), tumor necrosis factor-alpha (TNF-α), and interleukin-6 (IL-6) in serum, as well as the content of malondialdehyde (MDA) and reduced glutathione (GSH), the activities of catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPX) in the liver were determined using the kit assay method. Results: The optimal formula for TC-LBLF was 26% of LBLF, 2.2:1 of sorbitol-mannitol ratio, 11.2% of citric acid-aspartame, and 2.1:1 of citric acid-aspartame ratio. Compared with the MD group, the intervention of TC-LBLF effectively reduced the increase of organ index, the fasting blood glucose and insulin levels, serum TC and TG content, AST, ALT, inflammatory factors TNF-α and IL-6 levels, as well as the content of MDA in the liver. At the same time, it increased the content of GSH and the activities of CAT, SOD, and GPX in the mice's liver. TC-LBLF could improve the abnormal elevation of blood glucose and lipid in mice caused by HF diet, reduce inflammatory response, and alleviate liver peroxidation damage. Conclusion: TC-LBLF has a certain protective effect on HF induced liver injury in mice, which provides a theoretical basis for the application of LBLF in the development of functional foods.