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中国精品科技期刊2020

海湾扇贝糜水浴热诱导凝胶形成规律及性能分析

Effect of Gelation Temperature during Water Bath Heating on Gel Performance of Bay Scallop (Argopecten Irradians) Adductor Surimi

  • 摘要: 为研究扇贝糜热诱导凝胶性能,以海湾扇贝(Argopecten irradians)闭壳肌为原料,采用一段式(75、85、95、115、121 ℃)和二段式(35+95、45+95、55+95 ℃)水浴热诱导方式制备8组扇贝糜凝胶,测定其蛋白结构、质地和微观结构变化,并基于国际吞咽障碍食物框架(International Dysphagia Diet Standardization Initiative,IDDSI)对添加外源物的贝糜凝胶进行分级。结果表明,随一段式热诱导温度的升高,蛋白总巯基含量呈下降趋势,而45+95 ℃样品组显著性高于95 ℃样品组(P<0.05);热诱导导致蛋白二级和三级结构改变,α-螺旋、β-转角减少,β-折叠增加,无规则卷曲无显著变化,热诱导组中95 ℃样品表面疏水性最高,而45+95 ℃样品最低;贝糜热诱导凝胶的质构、凝胶强度、持水力和白度差异显著(P<0.05),45+95 ℃条件下贝糜凝胶的硬度、弹性、咀嚼性、内聚性、凝胶强度及持水性均最高;二段式热诱导更有利于贝糜形成致密、连续的微观网络结构;45+95 ℃贝糜凝胶符合IDDSI框架6级软质型及一口量产品特征,添加黄原胶、卡拉胶、TG酶、魔芋粉、蛋清均会显著(P<0.05)改善其持水性和蒸煮损失率,且添加2.5%黄原胶与添加5%黄原胶+2%卡拉胶的2组贝糜凝胶符合IDDSI框架中5级细馅型产品特征,添加2.5%黄原胶+1.5%卡拉胶的贝糜凝胶符合6级产品特征,均适用于吞咽困难人群。综上所述,海湾扇贝贝糜凝胶化过程易受热诱导方式的影响,45+95 ℃条件下形成的贝糜凝胶性能最强但质地偏软,结合卡拉胶和黄原胶可开发出适合吞咽困难患者不同需求的扇贝贝糜凝胶制品,为新型扇贝贝糜制品的开发提供了有价值的参考依据。

     

    Abstract: To investigate the effect of heating on gel properties of scallop muscle, eight groups of heat-induced gels from bay scallop (Argopecten irradians) adductor were prepared by the conventional one-step (75, 85, 95, 115, 121 ℃) or two-step (35+95, 45+95, 55+95 ℃) water bath heating. Protein structure, texture and microstructural changes in the heat-induced gels were determined. And many exogenous additives were selected for texture modifications with the aims to create potential applications as dysphagia-friendly foods based on bay scallop adductor. The results showed that the total sulfhydryl content of the gel protein tended to decrease with the increase of one-stage heating induction temperature, while the 45+95 ℃ sample group was significantly (P<0.05) higher than the 95 ℃ sample group. Heat induced changes in protein secondary and tertiary structure. The α-helix and β-turn of protein decreased, β-sheet increased, and irregular curl change had on significantly after thermal induction. In the heat-induced gel samples, the highest surface hydrophobicity was found in the 95 ℃ sample group, whereas the lowest was found in the 45+95 ℃ sample group. The TPA, gel strength, whiteness and water holding capacity of heat-induced gels were significantly (P<0.05) different. The hardness, elasticity, chewiness, cohesion, gel strength and water holding capacity of heat-induced gel formed at 45+95 ℃ were the highest. The two-stage heating mode is more conducive to forming a dense and continuous network gel structure for bay scallop adductor surimi. Furthermore, the International Dysphagia Diet Standardization Initiative (IDDSI) framework indicated that the heat-induced gel formed at 45+95 ℃ could be categorized as Level 6 (soft and bite-sized). The water-holding capacity and cooking loss rate of gel with xanthan gum, carrageenan, TGase, konjac flour and egg white were significantly (P<0.05) improved. Meantime, the gel samples with 2.5% xanthan gum and 5% xanthan gum+2% carrageenan could be categorized as level 5 (crumbly and moist) of IDDSI, and gel samples with 2.5% xanthan gum+1.5% carrageenan as level 6 of IDDIS. In conclusion, heating treatment at the appropriate temperature (45+95 ℃) could promote the formation of gel network, resulting in a soft-textural property. This study may provide a theoretical foundation for the creation and design of new dysphagia nutritional food products based on bay scallop adductor added with carrageenan and/or xanthan gum.

     

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