Abstract: To improve the stability of naringinase, a novel sodium alginate/polyethyleneimine/epoxy silane composite aerogel (SKP) was prepared in this study for the immobilization of naringinase. It showed that the aerogel had a three-dimensional porous structure and contained a large number of amino functional groups, which could effectively immobilize naringinase. The optimal process conditions for SKP were as follows: molecular weight of polyethyleneimine (PEI) of 10000 Da, addition amount of 0.3 g. When SKP was used for the immobilization study of naringinase, the optimal process conditions for the immobilized naringinase were obtained as follows: immobilization time of 6 h, immobilization temperature of 30 ℃, immobilization pH of 2.5, and the initial enzyme activity of 349.43 U/mL. The optimal reaction temperature for the immobilized naringinase was 50 ℃, and the optimal reaction pH was 4.0. After being reused of eight cycles, the immobilized nariginase still retained 60% of the initial enzyme activity. After being stored at 4 ℃ for 30 days, it still exhibited as high as 90% of the initial enzyme activity, while the free naringinase only retained 41.32% of the initial enzyme activity. The half-life of the enzyme activity of the immobilized naringinase was 10.46 h, which was significantly improved compared with that of the free naringinase (4.38 h). The enzyme loading rate, enzyme activity recovery rate and maximum enzyme activity of the immobilized naringinase were 94.65%, 81.65% and 673.68 U/g, respectively. Based on the results in this paper, it indicates that the SKP aerogel can be used for the immobilization of naringinase. After process optimization, the immobilized naringinase has excellent storage stability and good thermal stability, providing a new theoretical basis and application foundation for the industrial application of immobilized naringinase.