Abstract: Objective:To study the endophytic bacteria from Apocynum venetum L. and their antibacterial activity in order to find novel resources. Methods:Endophytic bacteria were isolated from Apocynum venetum L. by using the method of plate cultivation,and the antibacterial activity of the fermented metabolites was studied by the Oxford Cup method. Meanwhile,the target endophytic bacteria were identified by 16S rRNA sequence homologous analysis combined with their physiological and biochemical characteristics. Results:The fermentation broths of endophytic bacteria showed different antibacterial activity. The strain D1 had inhibitory effect on Escherichia coli,Staphylococcus aureus,Micrococcus lutea and Bacillus subtilis,the strain D3 had inhibitory effect on Micrococcus lutea and Bacillus subtilis. The D1 was identified to be Pseudomonas hunanensis,and D3 was identified to be Pantoea ananatis. Conclusion:The results showed that Apocynum venetum L. contains abundant endophytic bacteria resources with antibacterial activity,which could be further developed and utilized.