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中国精品科技期刊2020
潘承慧,齐东月,赵大云. 在体单向肠灌流模型检验大豆皂苷苷元的肠吸收特性[J]. 食品工业科技,2021,42(10):336−342. doi: 10.13386/j.issn1002-0306.2020090203.
引用本文: 潘承慧,齐东月,赵大云. 在体单向肠灌流模型检验大豆皂苷苷元的肠吸收特性[J]. 食品工业科技,2021,42(10):336−342. doi: 10.13386/j.issn1002-0306.2020090203.
PAN Chenghui, QI Dongyue, ZHAO Dayun. Determination of Soyasapogenols Absorption Characteristics in Rat Gut Using in Situ Single-pass Intestinal Perfusion Model [J]. Science and Technology of Food Industry, 2021, 42(10): 336−342. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020090203.
Citation: PAN Chenghui, QI Dongyue, ZHAO Dayun. Determination of Soyasapogenols Absorption Characteristics in Rat Gut Using in Situ Single-pass Intestinal Perfusion Model [J]. Science and Technology of Food Industry, 2021, 42(10): 336−342. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020090203.

在体单向肠灌流模型检验大豆皂苷苷元的肠吸收特性

Determination of Soyasapogenols Absorption Characteristics in Rat Gut Using in Situ Single-pass Intestinal Perfusion Model

  • 摘要: 目的:探究大豆皂苷苷元A和B的肠吸收特性。方法:建立HPLC色谱分析方法测定灌流液的苷元浓度,基于大鼠肠段的形态学变化考察在体单向肠灌流模型的可靠性,分别研究不同肠段(十二指肠、空肠、回肠、结肠)、苷元剂量和胆汁对大豆皂苷苷元肠吸收特性的影响。结果:大豆皂苷苷元在不同肠段的表观渗透速率(Papp)和吸收速率常数(Ka):空肠>十二指肠>回肠>结肠,苷元A在空肠段的Papp和Ka为15.34×10−5 cm/s和3.06×10−5 s−1(剂量50 mg/L),苷元B的Papp和Ka为11.36×10−5 cm/s和2.74×10−5 s−1(剂量50 mg/L);苷元A的吸收有显著浓度依赖性(P<0.05),而苷元B浓度依赖性不明显(P>0.05);有胆汁时,苷元A和B在空肠段的Papp分别为16.56×10−5和12.01×10−5 cm/s,Ka分别为3.06×10−5和2.97×10−5 s−1,均显著高于无胆汁组(P<0.05),胆汁的存在一定程度提高了肠部上端苷元的吸收。结论:大豆皂苷苷元主要吸收部位为空肠,苷元A吸收机制为被动扩散,胆汁的参与促进了吸收作用。

     

    Abstract: Objective: To investigate the absorption characteristics of soyasapogenol A and B. Methods: The effects of absorption sites (duodenum, jejunum, ileum, and colon), concentration and bile on the intestinal absorption of soyasapogenol were determined using HPLC analysis and in situ single-pass intestinal perfusion model. Results: The order of apparent absorption coefficient (Papp) and absorption rate constant (Ka) of soyasapogenol absorption sites were as followed: Jejunum>duodenum>ileum>colon. The Papp and Ka of soyasapogenol A at a concentration of 50 mg/L were 15.34×10−5 cm/s and 3.06×10−5 s−1 at jejunum, while soyasapogenol B were 11.36×10−5 cm/s and 2.74×10−5 s−1. The absorption capacity of soyasapogenol A was significantly concentration-dependent (P<0.05) while soyasapogenol B was not significant (P>0.05). The Papp of soyasapogenol A and B with bile were 16.56×10−5 and 12.01×10−5 cm/s, and the Ka were 3.06×10−5 and 2.97×10−5 s−1, which was higher than those without bile significantly (P<0.05). The presence of bile improved the absorption capability at upper part of the intestine to some extent. Conclusion: The absorption site of soyasapogenol was mainly at jejunum. The absorption mechanism of soyasapogenol A was passive diffusion, and the bile tended to have a positive impact on its intestinal absorption.

     

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