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中国精品科技期刊2020
张萌,王鹏,耿敬章,等. 基于HS-SPME-GC-MS比较分析不同原料对葱香油风味品质的影响[J]. 食品工业科技,2025,46(2):1−9. doi: 10.13386/j.issn1002-0306.2024020116.
引用本文: 张萌,王鹏,耿敬章,等. 基于HS-SPME-GC-MS比较分析不同原料对葱香油风味品质的影响[J]. 食品工业科技,2025,46(2):1−9. doi: 10.13386/j.issn1002-0306.2024020116.
ZHANG Meng, WANG Peng, GENG Jingzhang, et al. Comparative Analysis of Effects of Different Raw Materials on Flavour Quality of Scallion Oil Based on HS-SPME-GC-MS[J]. Science and Technology of Food Industry, 2025, 46(2): 1−9. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024020116.
Citation: ZHANG Meng, WANG Peng, GENG Jingzhang, et al. Comparative Analysis of Effects of Different Raw Materials on Flavour Quality of Scallion Oil Based on HS-SPME-GC-MS[J]. Science and Technology of Food Industry, 2025, 46(2): 1−9. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024020116.

基于HS-SPME-GC-MS比较分析不同原料对葱香油风味品质的影响

Comparative Analysis of Effects of Different Raw Materials on Flavour Quality of Scallion Oil Based on HS-SPME-GC-MS

  • 摘要: 为探究葱品种对炸制葱香油香气组成的影响,选用三种市售葱为原料,花生油为载体油脂,葱碎于130 ℃炸制3 min获得葱香油样本(大葱炸制油Fried Dacong Oil,DFO;红葱炸制油Fried Hongcong Oil,HFO及香葱炸制油Fried Xiaocong Oil,XFO),采用定量描述性感官分析(Quantitative descriptive sensory analysis,QDA)和顶空固相微萃取-气相色谱-质谱联用仪(Headspace-solid phase microextraction-gas chromatography-mass spectrometry,HS-SPME-GC-MS)对比分析不同样本间挥发性物质组成差异,并结合多元统计分析筛选造成3个葱香油之间感官属性强度产生差异的关键物质。感官评价结果表明相同工艺参数下所制备的葱香油,XFO表现出较强的葱香味和脂香味,浓郁度高,油腻和辛辣感较弱;GC-MS共分离鉴定出69种物质,利用正交偏最小二乘分析(Orthogonal partial least squares discrimination analysis,OPLS-DA)筛选出18种导致3种葱香油呈香特征产生差异的关键物质。相对香气活性值(Relative odor activity value,ROAV)分析结果表明,2-乙基-6-甲基吡嗪、丙醛、2,6-二乙基吡嗪、苯甲醛、愈创木酚、苯甲醇、5-甲基呋喃醛、2-乙酰基吡嗪、2-正戊基呋喃、3-乙基-2,5-二甲基-吡嗪、(E)-2-辛烯醛、2-吡咯甲醛、N-甲基-2-吡咯甲醛及2,3,5-三甲基吡嗪是引起葱香油样品风味品质差异的物质。与其他两种葱香油相比,XFO得到小组成员喜爱,符合对葱香油香气轮廓预期。本研究利用HS-SPME-GC-MS筛选出与葱香油相关的差异物质,可为我国后期高品质葱香调味油标准化加工奠定理论基础。

     

    Abstract: To explore the impact of different varieties of scallions on the aromatic profile of fried scallion oil, three commercially available scallion types were utilized as raw materials. Peanut oil served as the carrier fat, and the scallions were finely chopped and fried at 130 ℃ for 3 min to yield samples of scallion-flavored oil, denoted as Fried Dacong Oil (DFO), Fried Hongcong Oil (HFO), and Fried Xiaocong Oil (XFO). Quantitative descriptive sensory analysis (QDA) and headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) techniques were employed to examine and analyse the volatile composition among the samples. Furthermore, multivariate statistical analysis was conducted to identify key compounds contributing to variations in sensory attributes among the three types of scallion oils. The sensory evaluation results revealed that XFO displayed pronounced shallot aroma and fat flavor, coupled with high intensity and low levels of oiliness and pungency compared to scallion oil samples prepared under identical process parameters. A total of 69 substances were identified by GC-MS, and 18 key substances were pinpointed through orthogonal partial least squares analysis (OPLS-DA), elucidating the disparities in aroma profiles among the three scallion oils. Relative Odor Activity Value (ROAV) analysis highlighted specific compounds, including 2-ethyl-6-methylpyrazine, propionaldehyde, 2,6-diethylpyrazine, benzaldehyde, guaiacol, benzyl alcohol, 5-methylfurfural, 2-acetylpyrazine, 2-pentylfuran, 3-ethyl-2,5-dimethyl-pyrazine, (E)-2-octenal, 2-pyrrolecarboxaldehyde, N-methyl-2-pyrrolecarboxaldehyde, and 2,3,5-trimethylpyrazine as primary contributors to the distinct flavor profiles observed in the scallion-flavored oil samples. Notably, XFO garnered preference from the panelists relative to the other two scallion oils, aligning with the anticipated aroma profile of scallion oils. This study employed HS-SPME-GC-MS to discern distinct compounds associated with scallion oil, thereby laying a theoretical groundwork for standardized processing methods conducive to producing high-quality scallion oil in subsequent endeavors.

     

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