Science and Technology of Food Industry

ISSN 1002-0306
CN   11-1759/TS
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Determination on lycopene in functional foods by the high performance liquid chromatographic method[J]. Science and Technology of Food Industry, 2012, (17): 299-302. DOI: 10.13386/j.issn1002-0306.2012.17.014
Citation: Determination on lycopene in functional foods by the high performance liquid chromatographic method[J]. Science and Technology of Food Industry, 2012, (17): 299-302. DOI: 10.13386/j.issn1002-0306.2012.17.014
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Determination on lycopene in functional foods by the high performance liquid chromatographic method

    Abstract
  • The content of lycopene in functional foods was determined by the high performance liquid chromatographic method (HPLC) .The sample pre-treatment method was by use of water-oil two-phase extraction methods.Separation was achieved by the use of Inertsil ODS-SP column (5μm, 4.6×250mm) , acetonitrile:methanol (1:1) as mobile phase, a flow rate of 1.2mL/min and detector at 472nm was used.Lycopene showed good linear relationship between the range of 0.30 and 50.0μg/mL (R2=0.9998) ;and the average recovery was 98.6%with RSD=3.0%.The results showed that the method was accurate.
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