Study on extraction of seabuckthorn seed meal protein by alkaline extraction and enzymatic hydrolysis and on functional properties

Firstly, alkaline extraction and acid precipitation process of seabuckthorn seed meal protein (SSMP) was studied.The optimal parameters were determined as follows:pH10, ratio of seabuckthorn seed meal (SSM) to water 1:12, 60℃, 60min.Secondly, the residue was further hydroyzed by alcalase.The optimum condition of enzymatic hydrolysis was alcalase addition 240U/g, ratio of residue to water 1:10, 60℃, 60min.And its emulsifying activity and foaming activity were studied.The results showed that its functional propertises were affected by the environmental factors, such as alkali extraction pH, extraction temperature and ionic strengths.Most of indexes of functional properties at pH5.0 were the lowest.