A PCR-based method for construction of a genetic engineering Chinese rice wine yeast by disrupting the CAR1 gene

doi:10.13386/j.issn1002-0306.2012.17.030

A PCR-based method for construction of a genetic engineering Chinese rice wine yeast by disrupting the CAR1 gene[J]. Science and Technology of Food Industry, 2012, (17): 159-162. DOI: 10.13386/j.issn1002-0306.2012.17.030

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A PCR-based method for construction of a genetic engineering Chinese rice wine yeast by disrupting the CAR1 gene

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Gene targeting cassettes were synthesized by long flanking homology PCR (LFH-PCR) , a new cloningfree strategy to delete genes, and were transformed into yeast.A engineering Chinese rice yeast was constructed by disruption of CAR1 gene.Compared to the parental wild type, the engineering strain exhibited reduced production of EC and urea in Chinese rice wine.They were reduced by 72%and 38%, respectively.It was also suggested that Chinese rice wine brewed with the recombinant yeast does not affect overall fermentation profiles.

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A PCR-based method for construction of a genetic engineering Chinese rice wine yeast by disrupting the CAR1 gene

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