Science and Technology of Food Industry

ISSN 1002-0306
CN   11-1759/TS
High-quality sci-tech periodicals
Development and evaluation of indirect competitive enzyme-linked immunoassay for the parathion[J]. Science and Technology of Food Industry, 2012, (17): 163-165. DOI: 10.13386/j.issn1002-0306.2012.17.079
Citation: Development and evaluation of indirect competitive enzyme-linked immunoassay for the parathion[J]. Science and Technology of Food Industry, 2012, (17): 163-165. DOI: 10.13386/j.issn1002-0306.2012.17.079
shu

Development and evaluation of indirect competitive enzyme-linked immunoassay for the parathion

    Abstract
  • Polyclonal antibody (PcAb) against parathion was raised and used to develop an indirect competitive enzyme-linked immunoassay (icELISA) .The active eser method was employed to conjugate the hapten to bovin serum albumin (BSA) and ovalbumin (OVA) as immounogen and coating antigen respectively.Anti-parathion PcAb was used to develope icELISA.The result showed that the linear detection of this icELISA system was 3.39～ 533.05ng/mL, the detection limit was 0.90ng/mL, the average recovery of parathion from vegetables and water samples was 75.6%～127.4%.Therefore, the icELISA established was a pratical method for detection of parathion in real samples.
    • FullText(HTML)
  • loading
    • References (16)
    • Relative Articles
  • Supplements (0)

Catalog

    RSS Email Alert

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return