Antioxidant capacity of different ethanol extraction from three grains

The antioxidant capacity of different ethanol extraction from three grains (sorghum, purple rice, rhubarb rice) were evaluated. During the experiment, total phenols, DPPH· scavenging ability and total antioxidant capacity were measured with the water, 60%, 95% ethanol extractions of the three grains. Meanwhile, the mice blood cells hemolytic effect of 60% ethanol extracts of three grains was evaluated in vitro. The results showed that the total phenol content of sorghum water extraction was 2. 34mg/g (GAE/DW) , which was 4 times the content of purple rice and 12. 23 times that of rhubarb rice, respectively. DPPH· scavenging ability of sorghum water extraction was 43. 58%, however, DPPH· scavenging ability for the water extraction of purple rice and rhubarb rice was very low. In 60% ethanol concentration, the DPPH· scavenging ability of three grains were 89. 88%, 90. 16% and 64. 70%, respectively. The total antioxidant capacity, 0. 16mmol/g (TEAC/DW) , was 3. 99 times and 24. 89 times that of purple rice and rhubarb rice. The total phenol content of 95% ethanol extraction of rhubarb rice was 1. 38mg/g (GAE/DW) , total antioxidant capacity was strong, but the DPPH· scavenging ability was low. The inhibition rates of hemolytic effect of three grains were 50. 77%, 16. 73% and 22. 79% respectively at 2mg/mL. The results showed that sorghum could protect blood cells membrane modestly.