Research of extruded-ultra-micro pretreatment of aqueous enzymatic extraction of soybean oil technology

Based on single-factor experiments, five factors of extrusion temperature, rotatory speed, moisture, die diameter and particle size were chosen to evaluate in the present study. The response factor was oil extraction rate. By employing RSM design programmed, the optimized parameters were generated as follows:extrusion temperature 96℃, rotatory speed 96r/min, moisture 14. 6%, die diameter 15mm, particle size 120mesh, and oil extraction rate 94. 34%±0. 74%. Further analysis on the profile of secondary structure of soybean was carried out by using infrared spectroscopy method. The results illustrated that the oil extraction rate increased along with decreasing β-sheet content, increasd random coil content, and transferred unordered protein to ordered protein.