Isolation and identification of phenyllactic acid-producing LAB

doi:10.13386/j.issn1002-0306.2012.21.049

Isolation and identification of phenyllactic acid-producing LAB[J]. Science and Technology of Food Industry, 2012, (21): 192-195. DOI: 10.13386/j.issn1002-0306.2012.21.049

Citation:

Isolation and identification of phenyllactic acid-producing LAB[J]. Science and Technology of Food Industry, 2012, (21): 192-195. DOI: 10.13386/j.issn1002-0306.2012.21.049

Citation:

Isolation and identification of phenyllactic acid-producing LAB[J]. Science and Technology of Food Industry, 2012, (21): 192-195. DOI: 10.13386/j.issn1002-0306.2012.21.049

Isolation and identification of phenyllactic acid-producing LAB

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Abstract

By the anaerobic culture, 24 strains of lactic acid bacteria were obtained from pig’s caecum, small intestine, large intestine and feces, which could produce phenyllactic acid by HPLC. Seven strains produced 80～110mg/L phenyllactic acid (PLA) , and four strains could produce even more than 119mg/L PLA. Strain r16 from feces showed the highest PLA-producing ability (233. 0mg/L) . Based on 16S rDNA sequencing analysis, combining with its morphological, physiological, and biochemistry test, strain r16 and sain m15 were identified as Lactobacillus plantarum, and stain m343 and strain m462 as Enterococcus faecalis.

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Isolation and identification of phenyllactic acid-producing LAB

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