Study on extraction technology of total flavones using cellulase from leaves of winter-jujube

Total flavones in leaves of winter-jujube were extracted using cellulose.The total flavones extraction rate was determined by spectrophotometry method with rutin as reference substance.In the single factor analysis, the effect of extraction temperature, extraction time, enzyme amount and solid-liquid ratio were measured.The orthogonal test was used to optimize the conditions of extraction.The results showed that the best conditions of extraction total flavones from leaves of winter-jujube were the solid-liquid ratio 1:50 (g/mL) , extraction temperature 55℃, extraction time 90min, enzyme amount 4mg/g.Under this optimal condition, extraction rate was 2.45%.The results showed that the order of extraction rate of total flavones was as follows:extraction temperature>extraction time>enzyme amount>solid-liquid ratio.