Rapid detection of Pseudomonas cocovenenans by loop-mediated isothermal amplification

An assay using loop-mediated isothermal amplification ( LAMP) technology was developed for rapid detection of Pseudomonas cocovenenans. The 16S ～ 23S rRNA internal transcribed spacer ( ITS) sequence of Pseudomonas cocovenenans as target sequences, used to design LAMP primers.The LAMP mixture was made in 25μL of reaction mixture, also LAMP was used both in Pseudomonas cocovenenans and in tremella of Pseudomonas cocovenenans and we compaired the method with PCR.The result showed that a rapid technique for detection of Pseudomonas cocovenenans initially established by LAMP. The sensitivity of LAMP assay was 1000 times as high as PCR, the sensitivity of the LAMP was 5.4CFU /mL, and the detection limit of artificially contaminated tremella was 76CFU /g. The whole experimental procedure ( contain DNA purified, LAMP and UV transilluminator) can be completed in 2h. LAMP technology provides a simple, rapid and sensitive method for detection Pseudomonas cocovenenans in food.