Simultaneous determination of vancomycin and norvancomycin in milk by liquid chromatography-tandem mass spectrometry

doi:10.13386/j.issn1002-0306.2013.08.005

Simultaneous determination of vancomycin and norvancomycin in milk by liquid chromatography-tandem mass spectrometry[J]. Science and Technology of Food Industry, 2013, (08): 86-87. DOI: 10.13386/j.issn1002-0306.2013.08.005

Citation:

Simultaneous determination of vancomycin and norvancomycin in milk by liquid chromatography-tandem mass spectrometry

Abstract

Abstract

A simple, rapid and specific analytical methods for the determination of vancomycin and norvancomycin in milk had been developed based on liquid chromatography tandem mass spectrometry in a multiple reaction monitoring mode. Milk samples were extracted with 0.1% trifluoroacetic acid in water. Antibiotics in the extract were separated on a ZORBAX SB-C18 column using a gradient elution program of 0.1% formic acid aqueous solution and acetonitrile. Limits of detection for vancomycin and norvancomycin were 5μg/kg. Mean recoveries were 72.5%～87.2%, and the relative standard deviation (RSD) was less than 10%.

FullText(HTML)

References (7)

Relative Articles

Supplements (0)

Cited By

Science and Technology of Food Industry

Simultaneous determination of vancomycin and norvancomycin in milk by liquid chromatography-tandem mass spectrometry

Abstract

Catalog

Export File

Citation

Format

Content